- Original article
- Open Access
The Rice GLYCINE-RICH PROTEIN 3 Confers Drought Tolerance by Regulating mRNA Stability of ROS Scavenging-Related Genes
Rice volume 14, Article number: 31 (2021)
Plant glycine-rich proteins are categorized into several classes based on their protein structures. The glycine-rich RNA binding proteins (GRPs) are members of class IV subfamily possessing N-terminus RNA-recognition motifs (RRMs) and proposed to be involved in post-transcriptional regulation of its target transcripts. GRPs are involved in developmental process and cellular stress responses, but the molecular mechanisms underlying these regulations are still elusive.
Here, we report the functional characterization of rice GLYCINE-RICH PROTEIN 3 (OsGRP3) and its physiological roles in drought stress response. Both drought stress and ABA induce the expression of OsGRP3. Transgenic plants overexpressing OsGRP3 (OsGRP3OE) exhibited tolerance while knock-down plants (OsGRP3KD) were susceptible to drought compared to the non-transgenic control. In vivo, subcellular localization analysis revealed that OsGRP3-GFP was transported from cytoplasm/nucleus into cytoplasmic foci following exposure to ABA and mannitol treatments. Comparative transcriptomic analysis between OsGRP3OE and OsGRP3KD plants suggests that OsGRP3 is involved in the regulation of the ROS related genes. RNA-immunoprecipitation analysis revealed the associations of OsGRP3 with PATHOGENESIS RELATED GENE 5 (PR5), METALLOTHIONEIN 1d (MT1d), 4,5-DOPA-DIOXYGENASE (DOPA), and LIPOXYGENASE (LOX) transcripts. The half-life analysis showed that PR5 transcripts decayed slower in OsGRP3OE but faster in OsGRP3KD, while MT1d and LOX transcripts decayed faster in OsGRP3OE but slower in OsGRP3KD plants. H2O2 accumulation was reduced in OsGRP3OE and increased in OsGRP3KD plants compared to non-transgenic plants (NT) under drought stress.
OsGRP3 plays a positive regulator in rice drought tolerance and modulates the transcript level and mRNA stability of stress-responsive genes, including ROS-related genes. Moreover, OsGRP3 contributes to the reduction of ROS accumulation during drought stress. Our results suggested that OsGRP3 alleviates ROS accumulation by regulating ROS-related genes’ mRNA stability under drought stress, which confers drought tolerance.
RNA–binding proteins play important roles in cellular processes by regulating gene expression at post-transcriptional levels, which involves mRNA stability, RNA splicing as well as RNA transport. Since the plant glycine-rich (GR) proteins were first discovered in petunia 30 years ago (Condit and Meagher 1986), multi-functional roles of these proteins have been reported in development and response to several biotic and abiotic stimuli (Ciuzan et al. 2015; Magdalena and Michal 2018). Glycine-rich proteins are categorized into four classes based on their protein structures. The biggest difference among the four classes is that class I to III have a signal peptide (SP) while class IV has an RNA-recognition motif (RRM) or cold shock domain (CSD) at the N-terminus. Class I represents only a simple structure containing SP and GR motifs, and class II is similar to class I except for the cysteine-rich region at the C-terminus. Genes belonging to class I and II function in plant cell wall development and organ growth (Park et al. 2001; Ringli et al. 2001). Class III has an additional oleosins domain between SP and GR regions. The glycine-rich RNA binding proteins (GRPs) are members of class IV subfamily possessing N-terminus RRMs in addition to C-terminal GR regions, and proposed to be involved in post-transcriptional regulation of its target transcripts (Kang et al. 2013; Ciuzan et al. 2015; Magdalena and Michal 2018).
Diverse biological activities of plant GRPs have been reported over the last decade (Kim et al. 2007b; Kim et al. 2008; Streitner et al. 2010; Yang et al. 2014). Plant GRPs are involved in seed germination, seedling growth, flowering time, and stress tolerance (Fusaro et al. 2007; Kim et al. 2008; Kim et al. 2010; Löhr et al. 2014; Yang et al. 2014). The Arabidopsis AtGRP7, one of the most-well characterized GRPs, regulates gibberellin (GA)-mediated stem growth by down-regulating GA biosynthesis (Löhr et al. 2014). Also, the heterologous expression of AtGRP7 complemented the cold-sensitive phenotype of E. coli strain BX04, which lacks four cold shock proteins (Kim et al. 2007c). Similarly, overexpression of AtGRP7 increases the tolerance of plants against low temperatures and freezing stress in Arabidopsis (Kim et al. 2008). AtGRP7 also functions as a negative regulator for seed germination and seedling growth under dehydration and high salinity conditions (Kim et al. 2008).
To understand the molecular functions of GRPs, efforts to identify GRP-interacting RNAs were continued together with revealing the biological activity of GRPs. AtGRP7 possesses RNA chaperone activity and its N-terminal RRM and GR motifs are crucial for RNA chaperone activity (Kim et al. 2007c; Kwak et al. 2011). AtGRP7 preferentially interacts with U/C-rich RNA sequences (Meyer et al. 2017). Global transcript profiling also revealed that RNAs involved in biotic and abiotic stresses are accumulated in AtGRP7 overexpressing plants (Streitner et al. 2010). Through RNA-immunoprecipitation (RNA-IP) assay, AtGRP7 appears to bind with transcripts of stress- and antioxidant-related genes in vivo, including AtGRP7, COR15A/B, PR4, WRKY33, and MT2A (Meyer et al. 2017). Besides, AtGRP7 and AtGRP8 can bind to each other’s mRNAs, affecting alternative splicing of each other (Schoning et al. 2008).
RNA-binding proteins can also shuttle mRNA from the nucleus to the cytoplasm (Kedersha et al. 2005; Thanin and Julia 2018). In Arabidopsis, AtGRP2 and 7 are localized in both nucleus and cytoplasm and involved in mRNA export (Fusaro et al. 2007; Kim et al. 2008; Lummer et al. 2011). There is also evidence showing that GRPs coordinately regulate the cytoplasmic processing of the mRNA targets under stress conditions. In Arabidopsis, AtGRP2, 7, and 8 were isolated as major components of messenger ribonucleoprotein complexes (mRNPs) under oxidative stress conditions (Schmidt et al. 2010). In mammal, the glycine-rich domain mediates the formation of cytoplasmic mRNP aggregates such as processing bodies and stress granules in which mRNA degradation and storage occur (Wolozin 2012).
Compared with progress on molecular functions of Arabidopsis GRPs, there are relatively few achievements on the functional characterization of rice GRPs. Early studies were focused on the tissue-specific expression patterns of OsGRPs (Xu et al. 1995; Liu et al. 2003). Kim et al. (2010) investigated the functional redundancy of Arabidopsis and rice GRPs in cold adaptation responses. They found that OsGRP1, 4, and 6 possess RNA chaperone activity and play a role in the cold adaptation process. Heterologous expression of rice OsGRP4 and OsGRP6 rescued a defect in the export of mRNA caused by a mutation in AtGRP7 (Kim et al. 2010). These results suggest that GRPs in rice and Arabidopsis are functionally conserved and also function as RNA chaperones during the cold adaptation process. Different from their functional conservation in cold adaptation responses, GRP proteins play different functions in drought stress in Arabidopsis and rice. In Arabidopsis, AtGRP7 is a negative regulator of seed germination and seedling growth under dehydration and high salinity conditions (Kim et al. 2008). They also found that stomatal closure was delayed in AtGRP7 overexpressing plants under dehydration and high salinity conditions. Interestingly, overexpression of AtGRP7 in rice conferred drought tolerance at both vegetative and reproductive stages (Yang et al. 2014). These results brought up the possibility that the roles of GRPs in drought response is different in Arabidopsis and rice.
In this study, we functionally characterized the rice glycine-rich protein OsGRP3 and uncovered its roles in plants under drought stress. The expression of OsGRP3 was found to be induced by ABA as well as drought stress. OsGRP3 proteins form cytoplasmic foci upon exposure to ABA and mannitol treatments. Through a combination of transcript profiling and RNA-IP-coupled qRT-PCR analysis, we screened OsGRP3 target genes involved in ROS regulation and identified several OsGRP3-associating target mRNA transcripts. Furthermore, half-life analysis revealed that OsGRP3 has roles in regulating the stability of its target mRNA, thus altering the tolerance of plants to drought stress. Taken together, we show that OsGRP3 has important roles in drought tolerance by modulating the stability of its target transcripts involved in drought response and ROS regulation.
OsGRP3 Is a Drought-Inducible Glycine-Rich-RNA Binding Protein
To characterize the function of OsGRP3 in rice, we first analyzed the amino acid sequences of rice GRPs and compared it against publicly available Arabidopsis GRP sequences. The phylogenetic analysis formed a clade consisting of OsGRP3 and Arabidopsis AtGRP1, AtGRP7 and AtGRP8 (Fig. S1a). The RRM was highly conserved in both rice and Arabidopsis GRPs, but the length of the glycine-rich domain was diverse between the analyzed protein sequences (Fig. S1a). OsGRP3 possessed an RRM motif with a relatively longer glycine-rich domain (Fig. S1b). Among 6 annotated OsGRPs, OsGRP3 expression was significantly higher (Fig. 1a). GRPs are reported to show tissue- and developmentally-specific expression patterns (Magdalena and Michal 2018). Thus, we examined the expression patterns of OsGRP3 in various tissues at different developmental stages. OsGRP3 expression was detected in all tested tissues (Fig. 1b and S2). To investigate spatial expression patterns of OsGRP3 in detail, we generated transgenic rice plants expressing GFP under the control of the OsGRP3 promoter (pOsGRP3::GFP) (Fig. 1c). GFP fluorescence was widely detected in both leaves and roots of rice plants with high GFP fluorescence in guard cells (Fig. 1c). These data indicate that OsGRP3 is abundantly expressed in both leaves and roots of rice plants. We next analyzed expression patterns of OsGRPs in rice plants when exposed to drought conditions. OsGRP3 exhibited a positive response against drought (air-drying) (Fig. 1d) as well as OsGRP2 and OsGRP4 (Fig. S3). ABA treatments also triggered a positive response in OsGRP3 expression suggesting that its expression follows an ABA-dependent pathway (Fig. 1e). Taken together, these results indicate that OsGRP3 is highly expressed in rice plants and is further induced by drought and ABA treatments.
Overexpression of OsGRP3 Confers Drought Tolerance in Rice
To investigate the physiological functions of OsGRP3 in drought stress response, we generated transgenic plants overexpressing OsGRP3 (OsGRP3OE) and RNAi-mediated knockdown lines (OsGRP3KD). To generate gene specific knockdown transgenic plants, we used OsGRP3-specific fragment designed at 3′ untranslated region of 173 bps. Thirty independent transgenic lines from each expression system were screened and shortlisted normally growing plants to eliminate the possible effects of somaclonal variations. Based on the expression levels of OsGRP3, three single-copy lines were selected from each expression system for further analysis. The OsGRP3OE transgenic plants showed approximately 4-fold higher expression of OsGRP3 than non-transgenic (NT) control plants (Fig. 2a) while OsGRP3KD transgenic plants showed approximately 10-fold lower expression than in NT plants (Fig. 2). To evaluate the performance of the plants under drought conditions, selected transgenic plants (T3) and NT plants were grown in a greenhouse for 4 weeks and subjected to drought stress by withholding water for up to 3 days. Soil moisture content during drought treatments was monitored to make sure that stress treatments were uniformly applied (Fig. 2c). Drought-induced visual symptoms such as leaf rolling and wilting appeared earlier in NT than in OsGRP3OE transgenic plants (Fig. 2d and S4) while OsGRP3KD transgenic plants were indistinguishable from NT plants during drought treatments (Fig. 2d). After re-watering, OsGRP3OE plants started to recuperate from drought-induced damages while NT and OsGRP3KD plants continuously withered (Fig. 2d and S4). The enhanced tolerance of OsGRP3OE transgenic plants to drought stress was further supported by a higher survival rate compared to NT plants (Fig. 2e). Since drought stress negatively affects photosynthetic efficiency of plants (Pinheiro and Chaves 2010), the degree of drought tolerance was further analyzed by measuring photochemical efficiency (Fv/Fm) of photosystem II in plants. Fv/Fm value in NT plants started to decrease 1 day after drought treatments and continued to decrease during the drought treatments (Fig. 2f). OsGRP3OE plants, on the other hand, showed a slower decrease of Fv/Fm value than NT control plants during the drought treatments (Fig. 2f). Next, we applied the mild-drought condition by withholding water for up to 2 days to compare to drought phenotype between OsGRP3KD plants and NT plants. Drought-induced visual symptoms appeared earlier in OsGRP3KD plants than NT plants (Fig. 2g). The reduced tolerance of OsGRP3KD transgenic plants to drought stress was further supported by a significant lower survival rate compared to NT plants (Fig. 2h), and OsGRP3KD plants, on the other hand, showed a faster decrease of Fv/Fm value than NT control plants during the drought treatments (Fig. 2i). Taken together, these results indicate that overexpression of OsGRP3 improves and knock-down of OsGRP3 reduce drought tolerance in plants.
OsGRP3 Proteins Are Localized in RNA Processing Sites
Functions of RNA-binding proteins differ depending on their subcellular localization (Thanin and Julia 2018). To determine the subcellular localization of OsGRP3, we generated transgenic plants expressing OsGRP3-GFP fusion protein under the control of the OsCc1 constitutive overexpression promoter (OsGRP3-GFPOE). Drought tolerance of OsGRP3-GFPOE transgenic plants was confirmed so that GFP tagging did not disturb the function of OsGRP3 (Fig. S5). GFP fluorescence signals were detected in roots (Fig. 3a) and leaf nuclei (Fig. S5d) of the transgenic plants. We also observed GFP signals in the cytoplasmic membrane of both leaf and root tissues that is typical patterns shown by cytosolic proteins (Fig. 3a and S5d). To confirm the nuclear localization of OsGRP3, we expressed OsGRP3-GFP fusion protein under control of cauliflower mosaic virus 35S promoter (35S::OsGRP-GFP) in rice protoplasts together with OsNF-YA7-mCherry fusion protein as a positive control for nuclear localization (Lee et al. 2015). When OsGRP3-GFP was co-expressed with OsNF-YA7-mCherry in rice protoplasts, fluorescent signals of OsGRP3-GFP overlapped with OsNF-YA5-mCherry but it was also detected in cytosol confirming that OsGRP3 is localized in both nucleus and cytosolic region of plant cells (Fig. 3b).
Since environmental stresses affect not only the expression of RNA-binding proteins but also their localizations (Bogamuwa and Jang 2013; Jan et al. 2013), we investigated how OsGRP3 behaves when plants are exposed to stress conditions. When treated with ABA, GFP fluorescence of OsGRP3-GFP OE plants, which were highly concentrated in the nucleus, appeared to disperse into the cytoplasm and formed cytoplasmic foci both in roots (Fig. 3c and S6a) and leaves (Fig. S6b). These foci started to form when ABA concentration was over 10 μM (Fig. S6a). Similarly, OsGRP3-GFP OE plants also showed distinct foci formation in the cytoplasm after mannitol treatments (Fig. 3f).
Since the localization of OsGRP3 in cytoplasmic foci was only detected under stress conditions, we hypothesized that OsGRP3 is transported into processing bodies (P-bodies) or stress granules under stress conditions. P-bodies and stress granules are dynamic cytosolic aggregates induced under stress conditions that play important roles in the regulation of mRNA stability (Thanin and Julia 2018). To determine whether OsGRP3 is associated with either P-bodies or stress granules, we constructed vectors expressing two rice orthologs of Arabidopsis DECAPPING 1 (DCP1) (OsDCP1–1 and OsDCP1–2) fused to RFP (OsDCP1–1-RFP and OsDCP1–2-RFP) and a rice ortholog of Arabidopsis POLY (A) BINDING PROTEIN 8 (OsPABP8) fused to RFP (OsPABP8-RFP) for visualizing P-bodies and stress granules, respectively. We then co-transformed OsGRP3-GFP into rice protoplasts together with OsDCP1-RFP or OsPABP8-RFP. GFP fluorescence of OsGRP3-GFP clearly overlapped with RFP fluorescence of OsDCP1-RFP after ABA (Fig. 3d) and mannitol treatments (Fig. S7). These co-localization of OsGRP3 in protoplasts was further verified by using transgenic roots co-overexpressing OsGRP3-GFP and OsDCP1–1-RFP. Consistent with results from protoplasts, OsGRP3 and OsDCP1–1 were co-localized at cytoplasmic foci (P-body) upon ABA and mannitol treatments (Fig. 3e and f). Unexpectedly, no clear cytoplasmic foci formation of OsPABP8-RFP was detected in rice protoplasts after both ABA and mannitol treatments, while RFP fluorescence of OsPABP8-RFP was detected in cytoplasmic foci when the protoplasts were treated with heat stress (Fig. S8). Under heat stress, OsGRP3-GFP and OsPABP8-RFP showed co-localization in the cytosolic foci (Fig. S8). Collectively, these results suggest that OsGRP3 associated with P-bodies as well as stress granules in response to abiotic stresses.
Identification of Genes Regulated by OsGRP3
To identify the genes in the OsGRP3-mediated drought tolerance pathway, we performed RNA-sequencing analysis to isolate gene expression profiles in OsGRP3OX and OsGRP3KD plants compared with NT plants. A cutoff change of at least two-fold was used to isolate up- and down-regulated genes by OsGRP3. The analysis revealed that OsGRP3OX plants contained 195 up-regulated and 190 down-regulated genes compared with NT plants (Fig. 4a and Table S2). Among the 195 up-regulated transcripts, 45 genes belonged to the gene ontology (GO) category of response to abiotic and/or biotic stress and defense (Fig. 4b). The majority of these genes were categorized into defense- and stress-related genes and interestingly many pathogenesis-related (PR) genes were up-regulated (Fig. 4c and Table S3). This high frequency of defense- and stress-related genes affected by OsGRP3 overexpression suggests that OsGRP3 is closely associated with biotic and abiotic stress responses.
We then isolated genes showing opposite expression patterns between OsGRP3OX and OsGRP3KD plants as candidate genes directly regulated by OsGRP3 (Table S2 and S4). Through these criteria, we finally isolated 15 genes as target candidates in the OsGRP3-mediated drought tolerance pathway (Table 1). Interestingly, six genes showing a positive correlation with the expression level of OsGRP3 were associated with plant stress responses, including four PR genes, LATE EMBRYOGENESIS ABUNDANT PROTEIN 17 (LEA17), and EXOCYST SUBUNIT EXO70 FAMILY PROTEIN FX1 (FX1). On the other hand, eight genes showing negative correlation with OsGRP3 expression level included PEROXIDASE 1 (POX1), METALLOTHIONEIN 1d (MT1d), CAROTENOID 9, 10-CLEAVAGE DIOXYGENASE 1 (CCD1), 4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN (DOPA), LEUCINE-RICH REPEAT DOMAIN CONTAINING PROTEIN (LRR), LIPOXYGENASE (LOX) and S-ADENOSYLMETHOININE SYNTHETASE 1 (SAM1) that were associated with reactive oxygen species (ROS) regulations (Nisar et al. 2015; You and Chan 2015; Liang et al. 2019). These results indicate that OsGRP3 affects the gene expression involved in ROS regulation.
OsGRP3 Interacts with its Target RNAs and Regulates their Stability
Since GRP3 contained two conserved RNA-recognition motifs, which are crucial for interaction with RNAs (Streitner et al. 2012), we hypothesize that OsGRP3 forms an in vivo complex with its target mRNAs. To prove the hypothesis, we performed RNA-IP using transgenic rice plants expressing OsGRP3 translationally fused with MYC epitope under control of the GOS2 promoter (GOS2::MYC-OsGRP3; MYC-OsGRP3OE) to isolate in vivo OsGRP3-RNA complex. Expression patterns of the selected DEGs (Table 1) in MYC-OsGRP3OE plants and drought tolerance of transgenic plants were similar to that of OsGRP3OE plants (Fig. 5a and S5a) confirming that MYC epitope tagging did not disturb the function of OsGRP3. OsGRP3-RNA in vivo complex was then isolated by RNA-IP using MYC antibody. qRT-PCR analysis was performed to examine the enrichment of OsGRP3 on selected transcripts. Two of the 3 duplicate transcripts of PR1 genes were excluded since PR1 genes showed duplication in 3 separate loci in chromosome 7 producing three identical transcripts. Among the 6 genes positively regulated by OsGRP3, levels of PR5 transcripts were the highest in OsGRP3-RNA complex (Fig. 5b). On the other hand, transcripts of three genes negatively regulated by OsGRP3 were concentrated by immunoprecipitation of OsGRP3 (Fig. 5b). These were MT1d, DOPA and LOX transcripts with enrichment values of 12-, 7- and 5-fold higher than control, respectively. These results confirmed direct or indirect association of the OsGRP3 protein with target transcripts.
To further investigate the function of OsGRP3 in the regulation of its binding mRNAs, the half-lives of OsGRP3 bound transcripts (PR5, MT1d, DOPA, and LOX) were determined in OsGRP3OE, OsGRP3KD and NT plants (Fig. 5c, S9, and Table S5). The transcriptional inhibitor Cordycepin (3′ deoxyadenosine) was used to exclude the change of transcripts level caused by de novo transcription. There was no significant change in levels of all tested transcripts without cordycepin treatments, but the levels of the transcripts started to decrease after cordycepin treatments (Fig. 5c and S9). The half-life of PR5 transcripts in NT plants was 115 min and increased to 187 min in OsGRP3OE but decreased to 93 min in Osgrp3KD plants (Fig. 5c). In contrast, the half-life of MT1d was only 107 min in OsGRP3OE plants, while those were 187 and 250 min in NT and OsGRP3KD plants, respectively (Fig. 5d). Similarly, LOX transcripts were less stable in OsGRP3OE yet more stable in OsGRP3KD compared with NT plants (Fig. S9b and Table S5). DOPA transcripts, however, were more stable in OsGRP3OE plants despite its lower expression level in OsGRP3OE plants (Fig. S9a and Table S5). Collectively, these results show that OsGRP3 regulates its target mRNAs at the post-transcriptional level by affecting their stabilities.
Effect of Drought Stress on the Functions of OsGRP3
Since both the expression of OsGRP3 and its subcellular localization was altered under stress conditions (Figs. 1d and 3), we examined the effects of drought stress on the functions of OsGRP3 by monitoring the stability of its target mRNAs under drought conditions (air-drying). qRT-PCR analysis revealed that drought treatments induced expression of PR5 and MT1d but not DOPA and LOX genes in rice plants (Fig. 6a and S10). Thus, we focused on the regulation of PR5 and MT1d expression for explaining the functions of OsGRP3 under drought conditions. In OsGRP3OE plants, PR5 expression was higher than NT plants under normal conditions and further induced by drought treatments, while the drought-induced expression of PR5 was significantly reduced in OsGRP3KD plants. In contrast, drought inducible MT1d expression was lower in OsGRP3OE plants but higher in OsGRP3KD plants compared to NT plants (Fig. 6a). We then examined expression patterns of PR5 and MT1d in plants treated with both drought and cordycepin to determine the changes in the stability of these genes under drought conditions (Fig. 6b). The cordycepin treatments significantly reduced PR5 transcript levels in NT, indicating that transcription of the PR5 gene is successfully inhibited by the treatments. Similarly, PR5 transcripts were gradually reduced in OsGRP3KD plants. Different from NT and OsGRP3KD plants, PR5 transcripts were initially reduced but increased later in OsGRP3OE plants (Fig. 6b and Table S5). In contrast, the MT1d transcripts were more stable in OsGRP3KD plants but less stable in OsGRP3OE plants than NT plants under drought conditions (Fig. 6b and Table S5). We then examined the effect of drought treatments on the association of OsGRP3 with its target mRNAs. Drought treatments increased the transcript amount of PR5 but not to MT1d retrieved by RIP assay (Fig. 6c). Taken together, OsGRP3 differently regulates the stability of target mRNAs, and its regulation is affected by drought stress in rice plants.
ROS Accumulation Levels Are Altered in OsGRP3 Transgenic Plants under Drought Stress Conditions
Majority of target genes of OsGRP3 including LEA17, POX1, MT1d, CCD1, DOPA, and LOX are involved in regulation of ROS accumulation. Therefore, we checked the H2O2 accumulation levels in NT, OsGRP3OE, and OsGRP3KD plants under drought conditions. To examine H2O2 levels, leaves of the NT and transgenic lines subjected to air-drying were stained with DAB (Fig. 7a) and quantified by densitometric scanning of the DAB stained leaves (Fig. 7b). Histochemical staining showed that under normal conditions, transgenic lines had similar H2O2 levels to NT. Notable increase in ROS levels in NT was observed 2 h after air-drying (Fig. 7a). However, the leaves of OsGRP3OE showed significantly less accumulation of H2O2 (Fig. 7a and b; left panels) after air-drying. On the contrary, the leaves of OsGRP3KD showed more ROS accumulation than that of NT (Fig. 7a and b; right panels). When we measured the H2O2 concentration of the plants, H2O2 levels of OsGRP3OE and OsGRP3KD plants were significantly lower and higher than those of NT plants, respectively, after air-drying. These results are consistent with the observed drought tolerance of OsGRP3OE plants (Fig. 2). Taken together, OsGRP3 is involved in the regulation of H2O2 levels under drought stress conditions.
Characterization of glycine-rich RNA-binding proteins (GRPs) has revealed their important roles in development and response to environmental stresses (Lorkovic 2009; Magdalena and Michal 2018), but detailed molecular mechanisms governed by GRPs are still largely unknown. Potential involvement of GRPs in drought stress responses has been proposed in several studies, however, molecular mechanism of drought phenotype of GRPs still has been unexplored (Kim et al. 2008; Chen et al. 2010; Yao et al. 2016; Wang et al. 2018). Here we combined physiological and molecular approaches in elucidating the function of OsGRP3 under normal and stressed conditions. Overexpression of OsGRP3 significantly enhanced and knock-down of OsGRP3 reduced the drought tolerance of the transgenic plants (Fig. 2 and S4) suggesting that OsGRP3 function as a positive regulator in drought tolerance. Arabidopsis AtGRP7, which belongs to the same clade with OsGRP3 (Fig. S1), acts as a negative regulator of seed germination under dehydration conditions (Kim et al. 2008) and its heterologous expression in rice improves drought tolerance of the plants (Yang et al. 2014). Together with our findings, these results reinforced the idea that different GRP proteins regulate drought responses of plants in an opposite way.
Downstream genes up-regulated by OsGRP3 were categorized into two major groups: defense response and stress-related genes (Table 1 and Fig. 4). The defense response genes up-regulated by OsGRP3 overexpression included three WRKY transcription factors (OsWRKY45,79 and 62) and 19 PR genes (OsPR1, 2, 5, and 10) (Table S3). Similar to our data, expression of PR1, PR2, and PR5 expression was also up-regulated in Arabidopsis plants by overexpression of AtGRP7 (Streitner et al. 2010). Besides, heterogeneous overexpression of AtGRP2 and AtGRP7 also induced the expression of OsPR10a in rice plants and showed drought tolerance (Yang et al. 2014). These indicate that GRP-mediated regulation of PR genes is highly conserved in both Arabidopsis and rice plants.
So far, it has been known that WRKY and PR genes are main components of salicylic acid (SA) mediated plant defense mechanisms and also involved in plant abiotic stress mechanisms in many plants (Qiu and Yu 2009; Seo et al. 2011; Ali et al. 2018). OsWRKY45 and OsWRKY62 were identified as molecular interfaces of biotic and abiotic stress interaction (Seo et al. 2011) and overexpression of OsWRKY45 enhanced tolerance to both drought and biotic stresses (Qiu and Yu 2009). PR genes also conferred tolerance to drought when overexpressed in plants, as reported in several studies (Liu et al. 2013; Wu et al. 2016; Li et al. 2019). Critically, overexpression of Arabidopsis PR genes (PR1, PR2, and PR5) and rice OsPR10 improved the performance of plants under drought conditions (Wu et al. 2005; Liu et al. 2013). Moreover, mutation of NPR1, the master regulator of WRKY and PR gene expression, reduced drought tolerance in tomato (Li et al. 2019). These reports indicate that the observed drought tolerance in OsGRP3OE plants could be mainly achieved due to the up-regulation of WRKY and PR genes.
The stress-inducible genes up-regulated by OsGRP3 overexpression include OsLEA17 and rice PLASMA MEMBRANE PROTEIN 1 (OsPM1). LEA proteins are hydrophilic proteins involved in drought tolerance mechanisms of rice by increasing levels of osmolyte and soluble sugar and cell membrane stability, and by reducing ROS (Duan and Cai 2012; Kaur et al. 2017; Liang et al. 2019). OsPM1 mediates ABA influx through the plasma membrane for ABA-dependent guard cell closure in plants (Yao et al. 2018). Thus, the up-regulation of the stress-inducible genes by OsGRP3 overexpression may also constitute another mechanism to enhance drought tolerance.
Identifying the associated RNAs is crucial in understanding the molecular functions of RNA-binding proteins (RBPs). Higher enrichment of PR5 mRNA in RNA-IP analysis confirmed the physical association of OsGRP3 with PR5 mRNA (Fig. 5b). In addition, the stability of PR5 transcripts increased in OsGRP3OE but decreased in OsGRP3KD plants (Fig. 5c and Table S5) and under drought conditions than under normal conditions (Figs. 5c, 6b, and Table S5). Moreover, a significant increase in the retrieved transcript quantity of PR5 by OsGRP3 was detected under drought conditions (Fig. 6c). These results suggested that O
sGRP3 contributes to drought-induced stabilization of PR5 mRNAs. Similar to our findings, AtGRP7 positively regulates the expression of several PR genes in Arabidopsis, and RNA binding activity of AtGRP7 is required for the up-regulation of PR genes (Streitner et al. 2010; Hackmann et al. 2014). Besides, it was found that AtGRP7 physically associates with PR4 transcripts in Arabidopsis (Meyer et al. 2017). RBPs have been shown to function as regulators in post-transcriptional regulation of RNA metabolism, and some RBPs have RNA chaperone activity that prevents RNA misfolding or resolves misfolded RNAs (Kang et al. 2013). It has been reported that OsGRP3 exhibits RNA chaperone activity during cold adaptation process (Kim et al. 2010). Interestingly, the three up-regulated paralogs of PR1 in OsGRP3OE plants seemed to be indirect targets of OsGRP3 overexpression since no clear enrichment in RNA-IP was observed (Fig. 4b). However, it was reported that AtGRP7 also up-regulated PR1 transcript without direct binding (Meyer et al. 2017). Therefore, OsGRP3, directly and indirectly, regulate the stability of target mRNAs of PR genes to confer drought tolerance in rice.
Transcriptome analysis of OsGRP3OE and OsGRP3KD transgenic plants revealed that the DEGs negatively regulated by OsGRP3 are related to the regulation of antioxidant components such as metallothioneins and carotenoids in plants (Table 1). METALLOTHIONEIN genes encode low molecular antioxidants that regulate ROS under stress conditions (Zhu et al. 2009; Grennan 2011; Kumar et al. 2012; Han et al. 2014). CCD1, POX1, and LOX are involved in the regulation of cellular carotenoid content. CCD leads to the enzymatic turnover of C40 carotenoids into apocarotenoids which is a critical step in regulating carotenoid accumulation (Auldridge et al. 2006; Nisar et al. 2015). In addition, oxidation of carotenoid by POX1 and LOX is also required for maintaining carotenoid homeostasis in photosynthetic tissues (Auldridge et al. 2006; Walter and Strack 2011). Therefore, opposite expression pattern of these genes in OsGRP3OE and OsGRP3KD plants suggested that OsGRP3 directly or indirectly involved in regulating cellular ROS level response to drought stress and H2O2 accumulation results support this idea (Fig. 7). Various abiotic stresses increase cellular ROS levels of plants leading to oxidative stress (Gill and Tuteja 2010; You and Chan 2015). Previous reports showed that Arabidopsis atRZ-1a, a GRP containing zinc finger motif, positively regulates ROS accumulation under salt and dehydration stresses (Kim et al. 2007a). These results suggested that GRPs were closely involved in the regulation of ROS levels under stress conditions.
Among the genes negatively regulated by OsGRP3, OsMT1d, DOPA, and LOX mRNA showed physical interactions with OsGRP3 in RNA-IP analysis (Fig. 4b). MT and LOX genes have also been found as interactors of AtGRP7 in RNA-IP analysis (Meyer et al. 2017). Our RNA half-life analysis revealed that OsMT1d and LOX transcripts were more stable in OsGRP3KD transgenic plants and less stable in OsGRP3OE transgenic plants than NT control plants (Figs. 5c, 6b, and S10). It has not been characterized by how GRPs negatively regulates the stability of target mRNAs. RNA binding proteins involved in mRNA turnover are transported into P-bodies and stress granules under stress conditions in plants (Pomeranz et al. 2010; Jan et al. 2013). It was thought that the movement of RNA binding proteins mediate the transportation of mRNA into P-bodies or stress granules for post-transcriptional controls (Thanin and Julia 2018). OsGRP3 proteins were detected in both the nucleus and cytoplasm under unstressed conditions and found in P-bodies after ABA and mannitol treatments (Fig. 3, S6 and S7). These observations suggest that the negative regulation of MT1d and LOX stability is related to the association of interacting partners with OsGRP3 in P-bodies. Interestingly, OsGRP3 regulated its target mRNAs, PR5 and MT1d, oppositely. We could not find any proper reference in the literature for explaining such a dual mode of regulation. Our results showed that the amount of PR5 mRNA bound by OsGRP was significantly increased while that of MT1d mRNA remained similar after drought treatments (Fig. 6c). It appears that OsGRP3 binding affinity towards target mRNAs is being changed by drought treatments. It is therefore possible that the different changes in OsGRP3 binding affinity to its interactors under drought stress conditions are responsible for the dual mode of regulation.
In the present study, we provide physiological and molecular evidence indicating that the rice OsGRP3 modulates the expression of stress- and ROS-related genes. OsGRP3 transgenic plants showed that OsGRP3 negatively regulated ROS generation under drought condition compared to NT plants. We also identified that OsGRP3 physically interacts with its target mRNAs thereby regulating their stability. Taken altogether, OsGRP3 contributes to the enhanced tolerance of rice to drought stress by regulating mRNA stability of stress- and ROS related genes.
Plant Growth and Hormone Treatments
Rice seeds (Oryza sativa cv. Nipponbare) were sown on a Murashige–Skoog (MS) solid medium and incubated in the dark for 3 days at 28 °C. Then seedlings were transferred to a growth chamber with a light and dark cycle of 16 h light/8 h dark having a light intensity of 200 μmol m− 2 s− 1 and relative humidity of 70%. One-week-old seedlings were transferred to a liquid growth medium (Yoshida et al. 1976) and grown for 2 weeks for gene expression analysis. To examine ABA-dependent expression patterns of OsGRP3, seedlings were transferred to 50 ml tubes containing 100 μM ABA solution (Sigma, USA). For drought treatments, 3-week-old rice plants were air-dried at room temperature. Seedlings were harvested at indicated time points after ABA and drought treatment for RNA extraction.
Plasmids Construction and Rice Transformation
To generate OsGRP3 overexpressing plants, the coding sequence of OsGRP3 (Os03g0670700) was amplified from rice (Oryza sativa L. ssp. japonica cv. Nipponbare) total RNAs using the Reverse Transcription System (Promega, USA) and PrimeSTAR HS DNA polymerase (Takara, Japan). The amplified OsGRP3 coding sequence was cloned into rice transformation vector p700 carrying OsCc1 promoter for constitutive overexpression (OsCc1::OsGRP3) (Jang et al. 2002) and the final constructs were transformed into rice (Oryza sativa cv. Nakdong). For knock-down expression of OsGRP3, the inverted repeat gene cassette of OsGRP3-specific fragment was inserted into p700 carrying OsGOS2 promoter for constitutive overexpression (Jeong et al. 2010). The OsGRP3-specific fragment was designed at 3′ untranslated region of 173 bps in length and the final constructs were transformed into rice (Oryza sativa cv. Dongjin). To visualize the subcellular localization of OsGRP3 in rice plants, the coding sequence of OsGRP3 translationally fused with a green fluorescent protein (GFP) was inserted into p700 vector carrying the OsCc1 promoter (OsCc1::OsGRP3-GFP). To examine spatial expression patterns of OsGRP3 in rice plants, the upstream 1886 bps sequence from the transcriptional start site of OsGRP3 was amplified and used as an OsGRP3 promoter. OsGRP3 promoter was cloned into the p700 vector containing the GFP coding sequence (pOsGRP3::GFP). The final constructs were transformed into rice (Oryza sativa cv. Dongjin) by Agrobacterium (LBA4404) mediated co-cultivation, as described previously (Jang et al. 1999). Primers used for plasmids construction were listed in Table S1. Copy numbers of the transgenic plants were determined by TaqMan Q-PCR (Thermo Fisher, USA) using probes specific for the bar gene. To analyze the copy number of the transgenic rice plants, genomic DNA was extracted from 2-week-old rice seedlings. The genomic DNA extracted from transgenic plants previously confirmed as a single inserted homozygous line was used as a control for the single-copy insertion. The selected single-copy insertion lines were self-fertilized, and homozygous transgenic lines were selected from T2 generations by examining segregation rates on MS media containing phosphinothricin (Duchefa, Netherlands). Three independent single-copy inserted homozygous plants were selected from over thirty individual lines and propagated in a rice paddy field at Kyungpook National University, Gunwi (128:34E/36:15 N), Korea.
The GFP fluorescence of OsCc1::OsGRP3-GFP and OsCc1::OsGRP3-GFP / OsGOS2::OsDCP1–1-RFP transgenic rice plants was analyzed in leaves and 10 μM propidium iodide-stained roots of two-week-old transgenic plants using a Leica SP8 STED laser scanning confocal microscope (Leica, Germany). GFP and propidium iodide were excited at 488 nm, and then emission was detected between 512 and 560 nm for GFP and between 610 and 650 nm for propidium iodide, respectively. RFP was excited at 560 nm and then emission was detected between 580 and 620 nm.
RNA Isolation and Quantitative Real-Time PCR Analysis
Rice tissues harvested at indicated time points were used for total RNA extraction using the Hybrid-R RNA purification kit (GeneAll, Korea) according to the manufacturer’s instructions. First-strand complementary DNA (cDNA) was synthesized from 2 μg of total RNAs using RevertAid M-MuLV Reverse Transcriptase (Thermo Scientific, USA). Real-time PCR analysis was carried out using 2X Real-Time PCR smart mix (SolGent, Korea) and EvaGreen (SolGent, Korea) in an Mx3000P Real-Time PCR system (Stratagene, USA). The PCR reactions were performed by initial denaturation at 95 °C for 15 min, followed by 40 cycles of 95 °C for 20 s, 60 °C for 20 s, and 72 °C for 30 s. Rice Ubiquitin1 (Os06g0681400) was used as an internal control for normalization. Primers used for qRT-PCR analysis were listed in Table S1.
Rice Protoplast Preparation and Transient Gene Expression
Rice seedlings (Oryza sativa cv. Dongjin) were grown in the dark for 10 days and transferred to light conditions for 10 h. Rice protoplast preparation and transient gene expression were performed as described previously (Shim et al. 2018). For transient expression of OsGRP3-GFP in rice protoplasts, OsGRP3-GFP fusion protein sequence was amplified from the OsCc1::OsGRP3-GFP construct and inserted into the pHBT vector carrying 35 s promoter (Shim et al. 2018). Similarly, the coding sequence of OsDCP1–1, OsDCP1–2 and OsPABP8 omitting stop codon was inserted into a pHBT vector containing red fluorescent protein (RFP) for translation fusion of the inserts with RFP. Primers used for plasmid constructions were listed in Table S1. The constructs were then transformed into protoplasts using PEG-mediated transformation. The transformed protoplasts were treated with 0.1 mM ABA or 1.25 M mannitol and harvested 3 h after the treatments by centrifugation at 300 g for 2 min. The subcellular localization of OsGRP3 was observed by a Leica SP8 STED laser scanning confocal microscope (Leica, Germany).
Total RNAs were extracted from the leaf tissues of OsGRP3OE, OsGRP3KD, and non-transgenic (NT) plants that were grown under normal soil conditions for 4 weeks using the RNeasy plant mini kit (Qiagen, USA) according to the manufacturer’s instruction. cDNA libraries were prepared using the TruSeq RNA Sample Prep kit (v2) (Macrogen, Korea). Single-end sequences were obtained using IRGSP (v 1.0) and raw sequence reads were trimmed to remove adaptor sequence, and those with a quality lower than Q20 were removed using the Trimmomatic 0.32 software (Bolger et al. 2014). To map the reads to reference genome, all reads were assembled with annotated genes from the Rap-DB database [http://rapdb.dna.affrc.go.jp; IRGSP (v 1.0)] using TopHat software (https://ccb.jhu.edu/software/tophat/index.shtml). After mapping reads to a reference genome, differentially expressed genes (DEGs) were selected using a cut-off change of at least 2-fold change and Student’s t-test (P < 0.05). The selected DEGs were grouped by hierarchical clustering analysis (Complete Linkage).
The RNA-IP analysis was consulted as previously described with minor modifications (Keene et al. 2006; Peritz et al. 2006). Leaf tissues of 14-day-old rice seedlings were homogenized with liquid nitrogen, and the ground powder was incubated with polysome lysis buffer [100 mM KCl, 5 mM MgCl2, 10 mM HEPES pH 7.0, 0.5% Nonidet P-40, 1 mM DTT, RNase Out RNase inhibitor (Invitrogen, USA), 2 mM vanadyl ribonucleoside complexes solution (Sigma-Aldrich, USA), protease inhibitor cocktail (Roche, Switzerland)] for 20 min. The supernatant was separated from the crude extracts by centrifuging at 16,000 g for 20 min at 4 °C. After quantification of the soluble proteins by Bradford assay kit (Bio-rad, USA), 1 mg aliquot of the lysates was used for further analysis. Before the immunoprecipitation step, the lysates were pre-cleared with protein A-agarose beads equilibrated in lysis buffer containing 1 mg ml− 1 BSA (Sigma, USA) by rotating at 4 °C for 2 h. The aliquot of the pre-cleared lysate was stored and used as a control for total input of RNAs. After pre-clearing, the lysates were incubated with indicated antibodies at 4 °C for 6 h. The protein-RNA complexes were then precipitated using protein A-agarose beads. The beads were washed four times with 1st washing buffer [100 mM KCl, 5 mM MgCl2, 10 mM HEPES pH 7.0, 0.5% Nonidet P-40, 1 mM DTT] and additional four times with 2nd washing buffer [100 mM KCl, 5 mM MgCl2, 10 mM HEPES pH 7.0, 0.5% Nonidet P-40, 1 mM DTT, 1 M urea]. RNAs were eluted from the beads with the polysome lysis buffer containing 0.1% SDS and 30 μg of proteinase K at 50 °C for 30 min. Immunoprecipitated RNAs were purified using phenol/chloroform extraction and ethanol precipitation. Then, 0.01% of input RNAs were used to determine the relative enrichment of RNAs after immunoprecipitation.
Measurements of mRNA Half-Lives
The half-life of mRNA was determined in rice plants as previously described (Lidder et al. 2005; Park et al. 2012). Briefly, two-week-old rice seedlings were transferred soil to tap water for 2 days. After adaptation, the rice seedlings were treated with 1 mM of cordycepin (3′-deoxyadenosine) (Sigma, USA) through roots uptake for 30 min. After the treatment, the leaf tissues were harvested at indicated time points. RNA extraction, first-strand synthesis, and qRT-PCR were performed as described in “RNA Isolation and Quantitative Real-Time PCR Analysis.” Half-lives were calculated using Sigma plot software (Sigma Plot v10.0; http://www.systat.com).
Drought Stress Treatments and Tolerance Evaluation
Non-Transgenic (NT) (O. sativa cv. Nakdong for OsGRP3OE and O. sativa cv. Dongjin for OsGRP3KD), OsGRP3OE and OsGRP3KD plants were sown on MS solid medium and incubated in a dark growth chamber for 4 days at 28 °C. Seedlings were then transferred to a growth chamber with a light and dark cycle of 16 h light/8 h dark and grown for one additional day before transplanting into soil. Thirty plants from each line were transplanted into ten soil pots (4 × 4 × 6 cm: three plants per pot) within a container (59 × 38.5 × 15 cm) and grown for additional 4 weeks in a greenhouse (16 h light/ 8 h dark cycle) at 30 °C. Drought stress was imposed by sequentially withholding water for 3 days and re-watering for 10 days (Fig. 2). In case of air-drying drought treatment (Figs. 1d, 6, and 7), whole plants were exposed to air by removing the plants from their pots for several hours. Drought-induced symptoms were monitored by imaging transgenic and NT plants at the indicated time points after the drought treatments using a NEX-5 N camera (Sony, Japan). The soil moisture content was measured using the SM150 Soil Moisture Sensor (Delta-T Devices, UK). Transient chlorophyll a fluorescence was measured using the Handy-PEA fluorimeter (Hansatech Instruments, UK). Chlorophyll A fluorescence was measured from the longest leaves of each plant after 1 h of dark adaptation to ensure sufficient opening of the reaction center. Measurement was performed at apex, middle, and base regions of leaves using the Handy-PEA fluorimeter. Thirty measurements per line were averaged using the HANDY-PEA software (version 1.31).
Histochemical Staining and Measurement of H2O2
Accumulation of H2O2 was examined based on histochemical staining by 3, 3′-diaminobenzidine (DAB) as described before (Liu et al. 2012). NT and transgenic leaves of 2 weeks old plants were air-dried for different time intervals (1, 2, 3 h). These leaves were vacuum infiltrated into 1 mg/ml fresh DAB solution (pH 3.8) prepared in 10 mM phosphate buffer (pH 7.8) and incubated in dark cabinet overnight. The stained leaves were then fixed with a solution of 3:1:1 ethanol:lactic acid: glycerol and photographed. The H2O2 level was measured as described before (Kim et al. 2007a). The 2 weeks old plants (0.3 g fresh weight) were ground in 50 mM phosphate buffer (pH 6.8), and the homogenate was centrifuged at 6000 g for 25 min. To determine the H2O2 level, 3 ml of the extracted solution was mixed with 1 ml of 0.1% titanium chloride in 20% (v/v) H2O2, and the mixture was then centrifuged at 6000 g for 15 min. The absorbance of the supernatant was measured at 410 nm, and the H2O2 level was calculated based on the standard curve generated using authentic H2O2.
Genes from this article can be found in the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/) under the following accession numbers: GSE151351 (RNA-seq), GRP3 (Os03g0670700), PR5 (Os12g0629700), DCP1 (Os12g0156400), PABP8 (Os09g0115400), LEA17 (Os03g0322900), FX1 (Os05g0369900), POX1 (Os01g026330), MT1d (Os12g0571100), CCD1 (Os08g0371200), DOPA (Os01g0878900), LRR (Os01g0162300), LOX (Os10g0361000), SAM1(Os01g0293000).
Availability of Data and Materials
The datasets supporting the conclusions of this article are provided within the article and its additional files.
Glycine-rich RNA binding proteins
messenger ribonucleoprotein complexes
POLY (A) BINDING PROTEIN 8
LATE EMBRYOGENESIS ABUNDANT PROTEIN 17
EXOCYST SUBUNIT EXO70 FAMILY PROTEIN FX1
CAROTENOID 9, 10-CLEAVAGE DIOXYGENASE 1
4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN
LEUCINE-RICH REPEAT DOMAIN CONTAINING PROTEIN
S-ADENOSYLMETHOININE SYNTHETASE 1
reactive oxygen species
PLASMA MEMBRANE PROTEIN 1
Ali S, Ganai BA, Kamili AN, Bhat AA, Mir ZA, Bhat JA, Tyagi A, Islam ST, Mushtaq M, Yadav P, Rawat S, Grover A (2018) Pathogenesis-related proteins and peptides as promising tools for engineering plants with multiple stress tolerance. Microbiol Res 212-213:29–37. https://doi.org/10.1016/j.micres.2018.04.008
Auldridge ME, Block A, Vogel JT, Dabney-Smith C, Mila I, Bouzayen M, Magallanes-Lundback M, DellaPenna D, McCarty DR, Klee HJ (2006) Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme family. Plant J 45(6):982–993. https://doi.org/10.1111/j.1365-313X.2006.02666.x
Bogamuwa S, Jang JC (2013) The Arabidopsis tandem CCCH zinc finger proteins AtTZF4, 5 and 6 are involved in light-, abscisic acid- and gibberellic acid-mediated regulation of seed germination. Plant Cell Environ 36(8):1507–1519. https://doi.org/10.1111/pce.12084
Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30(15):2114–2120. https://doi.org/10.1093/bioinformatics/btu170
Chen X, Q-c Z, Lu X-p, Yu D-q, W-z L (2010) Characterization and expression analysis of four glycine-rich RNA-binding proteins involved in osmotic response in tobacco (Nicotiana tabacum cv. Xanthi). Agric Sci China 9(11):1577–1587. https://doi.org/10.1016/S1671-2927(09)60254-6
Ciuzan O, Hancock J, Pamfil D, Wilson I, Ladomery M (2015) The evolutionarily conserved multifunctional glycine-rich RNA-binding proteins play key roles in development and stress adaptation. Physiol Plant 153(1):1–11. https://doi.org/10.1111/ppl.12286
Condit CM, Meagher RB (1986) A gene encoding a novel glycine-rich structural protein of petunia. Nature 323(6084):178–181. https://doi.org/10.1038/323178a0
Duan J, Cai W (2012) OsLEA3-2, an abiotic stress induced gene of rice plays a key role in salt and drought tolerance. PLoS One 7(9):e45117. https://doi.org/10.1371/journal.pone.0045117
Fusaro AF, Bocca SN, Ramos RL, Barroco RM, Magioli C, Jorge VC, Coutinho TC, Rangel-Lima CM, De Rycke R, Inze D, Engler G, Sachetto-Martins G (2007) AtGRP2, a cold-induced nucleo-cytoplasmic RNA-binding protein, has a role in flower and seed development. Planta 225(6):1339–1351. https://doi.org/10.1007/s00425-006-0444-4
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48(12):909–930. https://doi.org/10.1016/j.plaphy.2010.08.016
Grennan AK (2011) Metallothioneins, a diverse protein family. Plant Physiol 155(4):1750–1751. https://doi.org/10.1104/pp.111.900407
Hackmann C, Korneli C, Kutyniok M, Koster T, Wiedenlubbert M, Muller C, Staiger D (2014) Salicylic acid-dependent and -independent impact of an RNA-binding protein on plant immunity. Plant Cell Environ 37(3):696–706. https://doi.org/10.1111/pce.12188
Han M, Kim CY, Lee J, Lee SK, Jeon JS (2014) OsWRKY42 represses OsMT1d and induces reactive oxygen species and leaf senescence in rice. Mol Cell 37(7):532–539. https://doi.org/10.14348/molcells.2014.0128
Jan A, Maruyama K, Todaka D, Kidokoro S, Abo M, Yoshimura E, Shinozaki K, Nakashima K, Yamaguchi-Shinozaki K (2013) OsTZF1, a CCCH-tandem zinc finger protein, confers delayed senescence and stress tolerance in rice by regulating stress-related genes. Plant Physiol 161(3):1202–1216. https://doi.org/10.1104/pp.112.205385
Jang IC, Choi WB, Lee KH, Song SI, Nahm BH, Kim JK (2002) High-level and ubiquitous expression of the rice cytochrome c gene OsCc1 and its promoter activity in transgenic plants provides a useful promoter for transgenesis of monocots. Plant Physiol 129(4):1473–1481. https://doi.org/10.1104/pp.002261
Jang I-C, Nahm BH, Kim J-K (1999) Subcellular targeting of green fluorescent protein to plastids in transgenic rice plants provides a high-level expression system. Mol Breed 5(5):453–461. https://doi.org/10.1023/A:1009665314850
Jeong JS, Kim YS, Baek KH, Jung H, Ha SH, Do Choi Y, Kim M, Reuzeau C, Kim JK (2010) Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions. Plant Physiol 153(1):185–197. https://doi.org/10.1104/pp.110.154773
Kang H, Park SJ, Kwak KJ (2013) Plant RNA chaperones in stress response. Trends Plant Sci 18(2):100–106. https://doi.org/10.1016/j.tplants.2012.08.004
Kaur R, Chakraborty A, Bhunia RK, Sen SK, Ghosh AK (2017) Tolerance to soil water stress by Oryza sativa cv. IR20 was improved by expression of Wsi18 gene locus from Oryza nivara. Biol Plant 62:129–139
Kedersha N, Stoecklin G, Ayodele M, Yacono P, Lykke-Andersen J, Fritzler MJ, Scheuner D, Kaufman RJ, Golan DE, Anderson P (2005) Stress granules and processing bodies are dynamically linked sites of mRNP remodeling. J Cell Biol 169(6):871–884. https://doi.org/10.1083/jcb.200502088
Keene JD, Komisarow JM, Friedersdorf MB (2006) RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts. Nat Protoc 1(1):302–307. https://doi.org/10.1038/nprot.2006.47
Kim JS, Jung HJ, Lee HJ, Kim KA, Goh C-H, Woo Y, Oh SH, Han YS, Kang H (2008) Glycine-rich RNA-binding protein7 affects abiotic stress responses by regulating stomata opening and closing in Arabidopsis thaliana. Plant J 55(3):455–466. https://doi.org/10.1111/j.1365-313X.2008.03518.x
Kim JS, Park SJ, Kwak KJ, Kim YO, Kim JY, Song J, Jang B, Jung CH, Kang H (2007c) Cold shock domain proteins and glycine-rich RNA-binding proteins from Arabidopsis thaliana can promote the cold adaptation process in Escherichia coli. Nucleic Acids Res 35(2):506–516. https://doi.org/10.1093/nar/gkl1076
Kim JY, Kim WY, Kwak KJ, Oh SH, Han YS, Kang H (2010) Glycine-rich RNA-binding proteins are functionally conserved in Arabidopsis thaliana and Oryza sativa during cold adaptation process. J Exp Bot 61(9):2317–2325. https://doi.org/10.1093/jxb/erq058
Kim JY, Park SJ, Jang B, Jung CH, Ahn SJ, Goh CH, Cho K, Han O, Kang H (2007b) Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions. Plant J 50(3):439–451. https://doi.org/10.1111/j.1365-313X.2007.03057.x
Kim YO, Pan S, Jung CH, Kang H (2007a) A zinc finger-containing glycine-rich RNA-binding protein, atRZ-1a, has a negative impact on seed germination and seedling growth of Arabidopsis thaliana under salt or drought stress conditions. Plant Cell Physiol 48(8):1170–1181. https://doi.org/10.1093/pcp/pcm087
Kumar G, Kushwaha HR, Panjabi-Sabharwal V, Kumari S, Joshi R, Karan R, Mittal S, Pareek SLS, Pareek A. Clustered metallothionein genes are co-regulated in rice and ectopic expression of OsMT1e-P confeavenging. BMC Plant Biol 2012;12:107. https://doi.org/10.1186/1471-2229-12-107
Kwak KJ, Park SJ, Han JH, Kim MK, Oh SH, Han YS, Kang H (2011) Structural determinants crucial to the RNA chaperone activity of glycine-rich RNA-binding proteins 4 and 7 in Arabidopsis thaliana during the cold adaptation process. J Exp Bot 62(11):4003–4011. https://doi.org/10.1093/jxb/err101
Lee DK, Kim HI, Jang G, Chung PJ, Jeong JS, Kim YS, Bang SW, Jung H, Choi YD, Kim JK (2015) The NF-YA transcription factor OsNF-YA7 confers drought stress tolerance of rice in an abscisic acid independent manner. Plant Sci 241:199–210. https://doi.org/10.1016/j.plantsci.2015.10.006
Li R, Liu C, Zhao R, Wang L, Chen L, Yu W, Zhang S, Sheng J, Shen L (2019) CRISPR/Cas9-mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance. BMC Plant Biol 19(1):38. https://doi.org/10.1186/s12870-018-1627-4
Liang Y, Kang K, Gan L, Ning SB, Xiong JY, Song SY, Xi LZ, Lai SY, Yin YT, Gu JW, Xiang J, Li SS, Wang BS, Li MT (2019) Drought-responsive genes, late embryogenesis abundant group3 (LEA3) and vicinal oxygen chelate, function in lipid accumulation in Brassica napus and Arabidopsis mainly via enhancing photosynthetic efficiency and reducing ROS. Plant Biotechnol J 17(11):2123–2142. https://doi.org/10.1111/pbi.13127
Lidder P, Gutierrez RA, Salome PA, McClung CR, Green PJ (2005) Circadian control of messenger RNA stability. Association with a sequence-specific messenger RNA decay pathway. Plant Physiol 138(4):2374–2385. https://doi.org/10.1104/pp.105.060368
Liu WX, Zhang FC, Zhang WZ, Song LF, Wu WH, Chen YF (2013) Arabidopsis Di19 functions as a transcription factor and modulates PR1, PR2, and PR5 expression in response to drought stress. Mol Plant 6(5):1487–1502. https://doi.org/10.1093/mp/sst031
Liu Z, Zhang Z, Faris JD, Oliver RP, Syme R, McDonald MC, McDonald BA, Solomon PS, Lu S, Shelver WL, Xu S, Friesen TL (2012) The cysteine rich necrotrophic effector SnTox1 produced by Stagonospora nodorum triggers susceptibility of wheat lines harboring Snn1. PLoS Pathog 8(1):e1002467. https://doi.org/10.1371/journal.ppat.1002467
Liu ZZ, Wang JL, Huang X, Xu WH, Liu ZM, Fang RX (2003) The promoter of a rice glycine-rich protein gene, Osgrp-2, confers vascular-specific expression in transgenic plants. Planta 216(5):824–833. https://doi.org/10.1007/s00425-002-0934-y
Löhr B, Streitner C, Steffen A, Lange T, Staiger D (2014) A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis. Mol Biol Rep 41(1):439–445. https://doi.org/10.1007/s11033-013-2878-7
Lorkovic ZJ (2009) Role of plant RNA-binding proteins in development, stress response and genome organization. Trends Plant Sci 14(4):229–236. https://doi.org/10.1016/j.tplants.2009.01.007
Lummer M, Humpert F, Steuwe C, Caesar K, Schuttpelz M, Sauer M, Staiger D (2011) Reversible photoswitchable DRONPA-s monitors nucleocytoplasmic transport of an RNA-binding protein in transgenic plants. Traffic 12(6):693–702. https://doi.org/10.1111/j.1600-0854.2011.01180.x
Magdalena C, Michal R (2018) Plant glycine-rich proteins in stress response: an emerging, still prospective story. Front Plant Sci 9:302
Meyer K, Köster T, Nolte C, Weinholdt C, Lewinski M, Grosse I, Staiger D (2017) Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7. Genome Biol 18(1):204. https://doi.org/10.1186/s13059-017-1332-x
Nisar N, Li L, Lu S, Khin Nay C, Pogson Barry J (2015) Carotenoid metabolism in plants. Mol Plant 8(1):68–82. https://doi.org/10.1016/j.molp.2014.12.007
Park AR, Cho SK, Yun UJ, Jin MY, Lee SH, Sachetto-Martins G, Park OK (2001) Interaction of the Arabidopsis receptor protein kinase Wak1 with a glycine-rich protein, AtGRP-3. J Biol Chem 276(28):26688–26693. https://doi.org/10.1074/jbc.M101283200
Park SH, Chung PJ, Juntawong P, Bailey-Serres J, Kim YS, Jung H, Bang SW, Kim YK, Do Choi Y, Kim JK (2012) Posttranscriptional control of photosynthetic mRNA decay under stress conditions requires 3′ and 5′ untranslated regions and correlates with differential polysome association in rice. Plant Physiol 159(3):1111–1124. https://doi.org/10.1104/pp.112.194928
Peritz T, Zeng F, Kannanayakal TJ, Kilk K, Eiriksdottir E, Langel U, Eberwine J (2006) Immunoprecipitation of mRNA-protein complexes. Nat Protoc 1(2):577–580. https://doi.org/10.1038/nprot.2006.82
Pinheiro C, Chaves MM (2010) Photosynthesis and drought: can we make metabolic connections from available data? J Exp Bot 62:869–882
Pomeranz MC, Hah C, Lin PC, Kang SG, Finer JJ, Blackshear PJ, Jang JC (2010) The Arabidopsis tandem zinc finger protein AtTZF1 traffics between the nucleus and cytoplasmic foci and binds both DNA and RNA. Plant Physiol 152(1):151–165. https://doi.org/10.1104/pp.109.145656
Qiu Y, Yu D (2009) Over-expression of the stress-induced OsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis. Environ Exp Bot 65(1):35–47. https://doi.org/10.1016/j.envexpbot.2008.07.002
Ringli C, Hauf G, Keller B (2001) Hydrophobic interactions of the structural protein GRP1.8 in the cell wall of protoxylem elements. Plant Physiol 125(2):673–682. https://doi.org/10.1104/pp.125.2.673
Schmidt F, Marnef A, Cheung MK, Wilson I, Hancock J, Staiger D, Ladomery M (2010) A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8. Mol Biol Rep 37(2):839–845. https://doi.org/10.1007/s11033-009-9636-x
Schoning JC, Streitner C, Meyer IM, Gao Y, Staiger D (2008) Reciprocal regulation of glycine-rich RNA-binding proteins via an interlocked feedback loop coupling alternative splicing to nonsense-mediated decay in Arabidopsis. Nucleic Acids Res 36(22):6977–6987. https://doi.org/10.1093/nar/gkn847
Seo Y-S, Chern M, Bartley LE, Han M, Jung K-H, Lee I, Walia H, Richter T, Xu X, Cao P, Bai W, Ramanan R, Amonpant F, Arul L, Canlas PE, Ruan R, Park C-J, Chen X, Hwang S, Jeon J-S, Ronald PC (2011) Towards establishment of a rice stress response interactome. PLoS Genet 7(4):e1002020. https://doi.org/10.1371/journal.pgen.1002020
Shim JS, Oh N, Chung PJ, Kim YS, Choi YD, Kim J-K (2018) Overexpression of OsNAC14 improves drought tolerance in rice. Front Plant Sci 9. https://doi.org/10.3389/fpls.2018.00310
Streitner C, Hennig L, Korneli C, Staiger D (2010) Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7. BMC Plant Biol 10(1):221. https://doi.org/10.1186/1471-2229-10-221
Streitner C, Koster T, Simpson CG, Shaw P, Danisman S, Brown JW, Staiger D (2012) An hnRNP-like RNA-binding protein affects alternative splicing by in vivo interaction with transcripts in Arabidopsis thaliana. Nucleic Acids Res 40(22):11240–11255. https://doi.org/10.1093/nar/gks873
Thanin C, Julia B-S (2018) Polysomes, stress granules, and processing bodies: a dynamic triumvirate controlling cytoplasmic mRNA fate and function. Plant Physiol 176:254–269
Walter MH, Strack D (2011) Carotenoids and their cleavage products: biosynthesis and functions. Nat Prod Rep 28(4):663–692. https://doi.org/10.1039/c0np00036a
Wang B, Wang G, Shen F, Zhu S (2018) A glycine-rich RNA-binding protein, CsGR-RBP3, is involved in defense responses against cold stress in harvested cucumber (Cucumis sativus L.) fruit. Front Plant Sci 9:540. https://doi.org/10.3389/fpls.2018.00540
Wolozin B (2012) Regulated protein aggregation: stress granules and neurodegeneration. Mol Neurodegener 7(1):56–56. https://doi.org/10.1186/1750-1326-7-56
Wu J, Kim SG, Kang KY, Kim J-G, Park S-R, Gupta R, Kim YH, Wang Y, Kim ST (2016) Overexpression of a pathogenesis-related protein 10 enhances biotic and abiotic stress tolerance in rice. Plant Pathol J 32(6):552–562. https://doi.org/10.5423/PPJ.OA.06.2016.0141
Wu KL, Guo ZJ, Wang HH, Li J (2005) The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res 12(1):9–26. https://doi.org/10.1093/dnares/12.1.9
Xu D, Lei M, Wu R (1995) Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation. Plant Mol Biol 28(3):455–471. https://doi.org/10.1007/BF00020394
Yang DH, Kwak KJ, Kim MK, Park SJ, Yang KY, Kang H (2014) Expression of Arabidopsis glycine-rich RNA-binding protein AtGRP2 or AtGRP7 improves grain yield of rice (Oryza sativa) under drought stress conditions. Plant Sci 214:106–112. https://doi.org/10.1016/j.plantsci.2013.10.006
Yao L, Cheng X, Gu Z, Huang W, Li S, Wang L, Wang Y-F, Xu P, Ma H, Ge X (2018) The AWPM-19 family protein OsPM1 mediates abscisic acid influx and drought response in rice. Plant Cell 30(6):1258–1276. https://doi.org/10.1105/tpc.17.00770
Yao LM, Jiang YN, Lu XX, Wang B, Zhou P, Wu TL. Overexpression of a glycine-rich protein gene in Lablab purpureus improves abiotic stress tolerance. Genet Mol Res 2016;15(4). https://doi.org/10.4238/gmr15048064
Yoshida S, Forno AD, Cock HJ, Gomez AK (1976) Laboratory manual for physiological studies of rice. International Rice Research Institute
You J, Chan Z (2015) ROS regulation during abiotic stress responses in crop plants. Front Plant Sci 6:1092
Zhu W, Zhao D-X, Miao Q, Xue T-T, Li X-Z, Zheng C-C (2009) Arabidopsis thaliana metallothionein, AtMT2a, mediates ROS balance during oxidative stress. J Plant Biol 52(6):585–592. https://doi.org/10.1007/s12374-009-9076-0
We thank the Rural Development Administration and Kyungpook National University for providing rice paddy fields.
This work was supported by a grant from the New breeding technologies development Program (Project No. PJ01477201 to J.-K.K.), Rural Development Administration, Republic of Korea.
Ethics Approval and Consent to Participate
Consent for Publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
. List of primers used in this study
. List of up- and down-regulated genes in OsGRP3OE transgenic plants compared to non-transgenic plants
. Up-regulated genes in OsGRP3OE transgenic rice plants in comparison with non-transgenic plants
. List of up- and down-regulated genes in OsGRP3KD transgenic plants compared to non-transgenic plants
. Analysis of half-life in non-transgenic, OsGRP3OE, and OsGRP3KD transgenic plants
. Phylogenetic analysis of OsGRP genes. Fig. S2. Expression levels of OsGRP3 in various rice tissues at different developmental stages. Fig. S3. Expression patterns of OsGRPs under drought conditions. Fig. S4. Drought tolerance of OsGRP3OE plants. Fig. S5. Drought tolerance of OsGRP3-GFPOE and MYC-OsGRP3OE plants and subcellular localization of OsGRP3 in leaves. Fig. S6. Effect of ABA treatments on subcellular localization of OsGRP3. Fig. S7. Effect of mannitol treatments on subcellular localization of OsGRP3 in rice protoplasts. Fig. S8. Effect of heat treatments on subcellular localization of OsGRP3 in rice protoplasts. Fig. S9. Effects of OsGRP3 on the stability of DOPA and LOX transcripts. Fig. S10. Expression patterns of OsGRP3, DOPA, and LOX under drought conditions
About this article
Cite this article
Shim, J.S., Park, SH., Lee, DK. et al. The Rice GLYCINE-RICH PROTEIN 3 Confers Drought Tolerance by Regulating mRNA Stability of ROS Scavenging-Related Genes. Rice 14, 31 (2021). https://doi.org/10.1186/s12284-021-00473-0
- Drought tolerance
- Cytoplasmic foci
- mRNA stability