Fig. 1

Expression of OsGRP3 in rice plants under drought conditions. a qRT-PCR analysis was performed for measuring endogenous expression levels of six annotated OsGRPs using RNAs extracted from leaves of two-week-old rice seedlings. b Expression levels of OsGRP3 in leaves and roots of rice plants at different developmental stages were analyzed using qRT-PCR. Rice UBIQUITIN (OsUBI) was used as an internal control for normalization. a and b Data represent the mean value ± standard deviation (SD) (n = 3, biological replicates). c The activity of OsGRP3 promoter was analyzed by detecting GFP fluorescence in two-week-old pOsGRP3::GFP transgenic plants. Bar = 30 μm. Two-week-old rice seedlings were exposed to d drought stress and e 0.1 mM ABA. Leaves of rice seedlings were harvested at an indicated time points after the treatments. Rice UBIQUITIN (OsUBI) was used as an internal control for normalization. d and e Data represent the mean value ± standard deviation (SD) (n = 3, biological replicates). Asterisks indicate a statistically significant difference compared with 0 h. *P < 0.05, **P < 0.01; One-way ANOVA