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Fig. 6 | Rice

Fig. 6

From: The Rice GLYCINE-RICH PROTEIN 3 Confers Drought Tolerance by Regulating mRNA Stability of ROS Scavenging-Related Genes

Fig. 6

Effects of drought on the function of OsGRP3. a Expression patterns of PR5 and MT1d were analyzed under drought conditions. Two-week-old non-transgenic plants (NT), OsGRP3 overexpressing (OsGRP3OE) and RNAi-mediated OsGRP3 suppressing (OsGRP3KD) transgenic plants were air-dried and harvested at indicated time points. Total RNAs isolated from harvested leaves were analyzed by qRT-PCR. Rice UBIQUITIN (OsUBI) was used as the internal control for normalization. b The stability of PR5 and Mt1d transcripts was analyzed under drought conditions. Two-week-old NT, OsGRP3OE and OsGRP3KD transgenic plants were pre-treated with distilled water or 1 mM cordycepin for 30 min. Plants were then treated with drought stress and harvested every 30 min after the treatments. Total RNAs extracted from the harvested samples were applied for qRT-PCR analysis. c Analysis of OsGRP3 interaction with PR5 and MT1d transcripts under drought conditions. For analyzing the interaction of OsGRP3 with PR5 and Mt1d transcripts, two-week-old MYC-OsGRP3OE plants were harvested 2 h after drought treatments and applied for RNA-IP experiments. NT plants were used as a negative control. Fold enrichment was normalized to the value of NT plants. a-c Data represent the mean value ± standard deviation (SD) (n = 3, biological replicates). a and c Asterisks indicate a statistically significant difference compared with NT. *P < 0.05, **P < 0.01; One-way ANOVA

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