Fig. 3

Change of subcellular localization of OsGRP3 in response to abiotic stresses. a Subcellular localization of OsGRP3 was visualized by detecting GFP fluorescence in roots of OsCc1::OsGRP3-GFP (OsGRP3-GFP^OE) transgenic plants under normal growth conditions. Bar = 30 μm. b OsGRP3-GFP and OsNF-YA7-mCherry were transformed into rice protoplasts. Fluorescence of GFP, mCherry, and chloroplast was analyzed using confocal microscopy. Bar = 10 μm. c GFP Fluorescence in roots of OsGRP3-GFP^OE transgenic plants was analyzed 6 h after treatment with ABA. Propidium iodide (PI) staining was used for visualizing outlines of root cells. Bar = 30 μm d Fluorescence of GFP, RFP and chloroplast were analyzed in the transformed protoplasts 6 h after ABA treatment using confocal microscopy. Bar = 10 μm. GFP Fluorescence in roots of OsGRP3-GFP^OE/ DCP1–1-RFP^OE transgenic plants was analyzed 6 h after treatment with ABA (e) and Mannitol (f). Bar = 30 μm. White arrows indicate co-localized sites of OsGRP3 and OsDCP1