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Fig. 5 | Rice

Fig. 5

From: The Rice GLYCINE-RICH PROTEIN 3 Confers Drought Tolerance by Regulating mRNA Stability of ROS Scavenging-Related Genes

Fig. 5

OsGRP3 affects the stability of its binding transcripts a Validation of expression levels of DEGs isolated from RNA sequencing analysis in non-transgenic plants (NT), MYC-OsGRP3 overexpressing (MYC-OsGRP3OE) and RNAi-mediated OsGRP3 suppressing (OsGRP3KD) transgenic plants by qPCR analysis. Total RNAs were isolated from leaves of two-week-old NT, MYC-OsGRP3OE and OsGRP3KD transgenic plants. Rice UBIQUITIN (OsUBI) was used as an internal control for normalization. b Analysis of OsGRP3 interaction with the transcripts of the selected DEGs. Leaves of two-week-old MYC-OsGRP3OE and NT plants were used for RNA-IP experiments. The anti-GFP antibody was used as a negative control for the anti-MYC antibody. Fold enrichment was normalized to the value of NT plants. a and b Data represent the mean value ± standard deviation (SD) (n = 3, biological replicates). Half-life measurement of c PR5 and d Mt1d transcripts. Two-week-old NT, OsGRP3OE and OsGRP3KD transgenic plants were pre-treated with 1 mM cordycepin for 30 min. Plants were then harvested every 30 min after mock or cordycepin treatments. Total RNAs extracted from the harvested samples were applied for qRT-PCR analysis. c and d Data represent the mean value ± standard deviation (SD) of three biological replicates. Half-lives of mRNA were calculated based on equation as shown in Table S5. Asterisks indicate a statistically significant difference compared with NT. **P < 0.01; One-way ANOVA

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