Phylogenomic Relationships of Rice Oxalate Oxidases to the Cupin Superfamily and Their Association with Disease Resistance QTL
© Springer Science + Business Media, LLC 2009
Received: 9 December 2008
Accepted: 2 February 2009
Published: 20 February 2009
Rice oxalate oxidase genes (OXO) may play a role in resistance to Magnaporthe oryzae. Genome analyses showed four tandemly duplicated OXO genes, OsOXO1–OsOXO4, which mapped to a blast resistance QTL in chromosome 3. These genes have >90% nucleotide and amino acid identity, but they have unique gene structures, conserved motifs, and phylogeny compared to the 70 other members of the cupin superfamily in the Nipponbare genome, which were divided into several classes. In resistant and susceptible Vandana/Moroberekan advanced backcross lines, only OsOXO4 was expressed during rice–M. oryzae interactions, and its expression increased earlier in resistant than susceptible lines. The earlier expression of OsOXO4 in resistant lines correlated with a 26-bp promoter insertion containing an additional copy of the bacterial responsive nodulation cis-element. Our results showed that OsOXO1–4 are in a separate class of rice cupin genes and supports a role for the promoter variant of OsOXO4 in resistance to M. oryzae.
One of the largest groups of proteins are cupins, which are a superfamily of functionally diverse but structurally related proteins (Dunwell et al. 2001; Dunwell et al. 2008). Two conserved motifs, ([G(X)5HXH-(X)3,4E(X)6G] and G(X)5PXG(X)2H(X)3N]), are shared by all cupins, and together make up the cupin domain. Two His residues and a Glu residue in Motif 1, together with a His residue in Motif 2, act as ligands for the binding of a manganese ion at the active site of the archetypal cupin, germin (Gane et al. 1998). Between the two cupin motifs is an intermotif region, that varies in length from 15 residues in many bacterial cupins to more than 50 residues in some plant cupins, and the number of residues in the intermotif region have been used to define at least 18 subclasses of cupins from archaea, bacteria, and eukaryotes (Dunwell et al. 2000). In addition to subclasses, cupins can be divided into two groups, the monocupins and bicupins (Dunwell et al. 2004). Monocupins, such as bacterial phosphomanose isomerases (PMIs), bacterial AraC transcriptional regulators, plant oxalate oxidases (OXO), and plant germins, have a single copy of the cupin domain, while bicupins, such as the plant seed storage proteins and plant oxalate decarboxylases, have two copies (Khuri et al. 2001).
Germin was first discovered in wheat during the onset of germination and was later determined to have similarity with barley OXOs that are responsible for the degradation of oxalate (Lane et al. 1993). Cereal germins and OXOs cluster together in phylogenetic analyses and are considered to be the true germins (Carter and Thornburg 2000; Khuri et al. 2001). OXOs may be important in disease resistance because they degrade oxalate, and oxalate is a virulence factor produced by several fungal pathogens (Bateman and Beer 1965; Godoy et al. 1990; Cessna et al. 2000; Guimaraes and Stotz 2004; van Kan 2006). In addition to degrading oxalate, OXO activity generates H2O2 from the reaction of oxalate + O2 + 2 H+ = 2 CO2 + H2O2 (Lane 1994). Active oxygen species, such as H2O2, are reported to be involved in plant defense responses (Lamb and Dixon 1997). This can be through oxidative cross-linking of lignin and proteins during cell wall modification, activating gene expression for transcription-dependent defenses, and acting directly as antimicrobial agents to inhibit pathogen development (Lamb and Dixon 1997). OXO-generated H2O2 may also function as a secondary messenger in the activation of phytoalexin biosynthetic pathways, hypersensitive response (HR), systemic acquired resistance, and PR gene expression in plants, all of which have been related to disease resistance (Carter et al. 1998).
Genes with sequence similarity to germins are termed germin-like proteins (GLPs), which have sequence similarity to OXO but are not true germins (Membre et al. 1997; Membre et al. 2000; Khuri et al. 2001). GLPs have a variety of functions (Zhou et al. 1998; Faris et al. 1999; Druka et al. 2002; Ramalingam et al. 2003; Liu et al. 2004; Wu et al. 2004; Manosalva et al. 2008). For example, a GLP, PSGER1, in peas shows sequence similarity to the N-terminal sequence of a putative plant receptor for rhicadhesin, a bacterial attachment protein (Gucciardo et al. 2007). Such rhicadhesin receptor-like GLPs have been found to be strongly induced in roots and root cultures of Medicago truncatula colonized by the arbuscular-mycorrhiza (AM) fungus, Glomus intraradices (Doll et al. 2003). Another example of a GLP is the Nectarin I precursor protein, which is located in floral nectaries of tobacco where it exhibits superoxide dismutase activity that generates high levels of hydrogen peroxide in the nectar and thus may function in plant defense (Carter and Thornburg 2003). Other GLPs have also been implicated in a variety of plant–microbe interactions. For example, an OXLP was associated with papilla formation in barley during infection with Blumeria (Erysiphe) graminis f.sp. hordei (Bgh) (Wei et al. 1998).
OXOs and GLPs have been implicated in quantitatively inherited disease resistance in rice (Faris et al. 1999; Ramalingam et al. 2003; Liu et al. 2004; Wu et al. 2004). In some rice germplasms, GLPs contributed to a major effect QTL on chromosome 8. Gene silencing studies recently demonstrated that a cluster of chromosome 8 GLPs contributed to broad-spectrum, basal disease defense responses (Manosalva et al. 2008). In other germplasms, OXOs were the major contributors to rice blast resistance (Ramalingam et al. 2003; Wu et al. 2004). OXO was mapped to a QTL in chromosome 3 of rice and was also shown to contribute to variation in number of rice blast lesions in an advanced backcross population derived from rice cultivars Vandana and Moroberekan (Wu et al. 2004). Moroberekan is a tropical japonica variety from Africa that is highly resistant to rice blast (Wang et al. 1994) while Vandana is a rice blast-susceptible cultivar from India (Wu et al. 2004). Thus, OXO genes have been associated with blast resistance.
Although members of the cupin gene family are important in a variety of plant functions, including disease resistance, only a few specific gene family members had previously been studied in rice and no systematic phylogenetic analysis of the family was available. The publication of a high quality, complete genome sequence for rice (IRGSP 2005) enabled a characterization of the entire cupin gene family of rice. In the process, we identified four OXO genes (OsOXO1, OsOXO2, OsOXO3, and OsOXO4) on chromosome 3 of the Oryza sativa ‘Nipponbare’ genome that co-localized with a previously reported blast disease resistance QTL (Ramalingam et al. 2003; Wu et al. 2004). All four OXOs exhibited similar structural gene sequences, but the promoter regions were distinct from each other. To determine if promoter differences could account for phenotypic differences in blast disease resistance, we compared promoters of the four genes from both Moroberekan and Vandana and progenies from an intermated population. We demonstrated that only the promoters of OsOXO4 from resistant varieties contained an extra element suggesting differential regulation of the genes. Consistent with this prediction, we show a correlation with OsOXO4 expression and disease resistance.
Phylogenomic analysis of rice cupins
While a number of monocupin genes occurred as singletons in the O. sativa cv. Nipponbare genome, most members were in eight clusters of two or more genes on chromosomes 1, 3, 4, 5, 8, 9, and 12. Clustering on chromosomes correlated with the monocupin subclass (Fig. 1) and often occurred in tandem arrays. All four OXO genes in rice are tandemly arrayed on chromosome 3 and have >90% AA identity with most of the variation at the 5’ end (Supplementary Fig. 1). Similarly, the six rhicadhesin receptor-like GLP genes occurred as a tandem pair on chromosome 1, two singletons on chromosome 1, and a singleton each on chromosome 3 and 5. The nectarin 1 precursor-like protein genes occurred as three tandem genes on chromosome 3 and a singleton in chromosome 1, the ABPLs occurred as tandem pair on chromosome 8 and a singleton on chromosome 4, and the ‘unknown function’ GLPs were a tandem pair on chromosome 9 with three singletons on chromosomes 2, 3, and 5. The OXLPs were three tandem genes on chromosomes 12 and 2, and 11 tandem genes on chromosome 8, as well as two singleton OXLPs on chromosomes 4 and 8. The singleton (LOC_Os08g13440) in chromosome 8 is 2.7 Mb downstream of the cluster of 11 tandem OXLP genes and groups phylogenetically with two OXLPs in the cluster (Fig. 1).
The high occurrence of tandem arrays indicates gene duplication, which is supported by all the genes being oriented in the same direction in the eight clusters with two or more monocupin genes. One exception may be the singleton OXLP (LOC_Os08g13440) on chromosome 8. Although LOC_Os08g13440 has ∼80% AA identity with the OXLPs in the tandem array, it is oriented in the opposite direction (as shown in Supplementary Table 2).
Protein alignment and analyses of the rice monocupins
While motifs 1–8 defined the cupin domain, motifs 9–25 did not define another cluster of orthologous gene (COG) domain. Rather, these motifs corresponded to the subclasses identified in Fig. 1 that corresponded to the SWISS-PROT annotations. Certain motifs or combinations of motifs were unique to subclasses of monocupins (Fig 2; as shown in Supplementary Table 3). Motif 9 is only found in nectarin I precursor-like proteins and OXLPs. Motif 11 is only found in OXLPs in chromosomes 8 and 12 but not in the three OXLPs on chromosome 2. Motifs 13, 14, and 16 are unique among OXOs, and motif 15 is unique to the ABP-like proteins and rhicadhesin receptor-like proteins. Motifs 17 and 19 are found only in the GLP subclass with unknown functions.
Some motifs are unique to cereals, i.e., motifs 9–12 and 17–19 were identified only in cereal GLPs and motifs 13–14 and 16 were found only in cereal OXOs. On the other hand, motifs 15 and 20 were annotated as ABP/adenosine diphosphate glucose pyrophosphatase (ADP) (as shown in Supplementary Table 3), which is common to germins in all flowering plants (Rodriguez-Lopez et al. 2001). Other motifs were not unique to cupins, i.e., motifs 21 and 23–25 matched hypothetical proteins, while motif 22 is similar to a retrotransposon gag protein (Marchler-Bauer et al. 2005).
All monocupins contain a signal peptide, except LOC_Os03g58980 and LOC_Os03g58990, which are annotated as nectarin 1 precursor protein GLPs (as shown in Supplementary Table 4). The OXOs and GLPs have distinct signal peptides (motif 14 in the OXOs and motif 10 in the OXLPs; Fig. 2). Signal peptides predicted by SignalP for the monocupins in the other subclasses were not well enough conserved to be identified as motifs by MEME analysis. All monocupins were predicted by Wolf pSORT to be located in the extracellular matrix. The only monocupins with the same signal peptide sequence were OsOXO3 and OsOXO4 (as shown in Supplementary Fig. 1).
Hydrophobicity profiles of the monocupins correspond with the motifs identified by MEME analysis (Fig. 2). A hydrophobic peak corresponding to the signal peptide sequence is followed by a relatively neutral region and then a series of three or more hydrophobic peaks corresponding to motifs 3, 1, 7, and 2. Hydropathy profiles were similar within subclass members, except for LOC_Os02g29010 and LOC_Os04g22080, which were in the OXLP subclass and have transposon insertions (Fig. 2). LOC_Os03g58990 in the nectarin I precursor-like protein subclass (Fig. 1) lacks several motifs and also has a hydrophobicity profile distinct from the rest of the monocupins. The predicted isoelectric points for the monocupin proteins range from 5.12–9.48, but differences in pI did not correspond to any of the monocupin subclasses (Fig. 2). A 1–2 N-glycosylation is a characteristic of all monocupins (Membre et al. 2000) and was found for all the rice monocupins, except LOC_Os04g52720 and LOC_Os8g35750, which have 1–4 N-glycosylation sites (as shown in Supplementary Table 4).
Gene structure of monocupins
Of the 45 monocupin genes, 15 have no intron, 27 have one intron, and three have multiple introns (as shown in Supplementary Fig. 3). Interestingly, none of the four OXOs have introns, and the rhicadhesin receptor-like proteins all have one intron. The presence of untranslated regions (UTRs) varied among the monocupin genes (as shown in Supplementary Table 5). However, a search using KOME (http://cdna01.dna.affrc.go.jp/cDNA/) showed that full-length cDNAs are only available for the 19 monocupin genes with both 5’ and 3’ predicted UTRs. A search of the GenBank EST database dbEST (http://www.ncbi.nlm.nih.gov/dbEST/index.html) containing over 28,262,948 ESTs showed that genes with both 5’ and 3’ predicted UTRs have many more ESTs (average = 65 ESTs) than genes with either a 5’ or 3’ UTR only (average = 6 ESTs) or no UTRs at all (average = 3 ESTs) (as shown in Supplementary Table 5).
Selection pressure among monocupins
From the ratio of synonymous to nonsynonymous substitution, selection pressure could not be detected among monocupin genes in the different subclasses, except for the rhicadhesin-like receptor protein, OXOs and OXLPs (Fig. 1). Diversifying selection was detected in rhicadhesin-like receptor protein subclass (ω = 1.1257), while the OXOs are under purifying selection (ω = 0.1361) (Yang et al. 1998). The OXLPs are divided into three groups based on phylogenetic clustering (Fig. 1), but all three groups are under purifying selection (ω = 0.1397 0.2072 and 0.1273).
OXOs are unique in cereals
Comparison of OXO sequences in resistant and susceptible rice-blast interactions
Field Blast Resistance of Moroberekan, Vandana, and Selected Progenies in India and the Philippines, 2004
Leaf blast score (0–9)a,b
Los Baños, Philippines
We observed several nucleotide differences in the OXO genes between cvs. Moroberekan and Vandana that resulted in AA differences. In OsOXO1, there was a difference at AA 123 with an L in Moroberekan and a V in Vandana (as shown in Supplementary Fig. 4). In OsOXO2, a CG insertion at nucleotide position 122 in Vandana results in a stop codon for Vandana, but not for Moroberekan, making its protein 174 AA shorter (as shown in Supplementary Fig. 5). OsOXO3 was identical in Moroberekan and Vandana. In OsOXO4, there was a difference of F to Y at AA 37, V to D at AA 46, and V to I at AA 117 between Moroberekan and Vandana, respectively (as shown in Supplementary Fig. 4). The difference at AA117 is in motif 1 of the cupin domain. For all four OXO genes, the resistant progenies, IR78221-19-6-7-B, IR7822-19-6-56-B, and IR78221-19-6-99-B, have the same sequence as Moroberekan, while the susceptible progeny, IR78221-19-3-196-B, has the same sequence as Vandana.
Expression of the OsOXO4 is correlated with quantitative disease resistance to blast
Fifteen EST libraries from the DFCI Rice Gene Index (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=Rice) were surveyed for the expression of each OXO gene by BLAST. Each gene had different temporal and spatial expression patterns except for OsOXO2 where no EST was detected under any condition (as shown in Supplementary Fig. 6). OsOXO1 was expressed in the panicles and at the flowering and pollination stages. OsOXO3 ESTs were found in roots and seed libraries. OsOXO4 ESTs were detected in drought, cold stress, and metal (CuSO4) stress libraries, as well as in healthy root, shoot, and leaf libraries. It was also expressed at tillering and booting stages (as shown in Supplementary Fig. 6). These results are consistent with a role for OsOXO4 in rice stress in general.
Most of the known genes involved in QTL-based defense responses are members of complex gene families. This poses difficulties in utilizing them in practical plant breeding. The approach described in this work to elucidate the functional OXO in rice resistance to M. oryzae combined genomic information with phenotypic selection, allowing us to narrow down the target chromosomal region for disease resistance and to identify features that distinguish that gene from other members of the cupin superfamily in rice. A phylogenetic analyses of the OXOs in relation to the cupins in rice showed that it is a sizable gene family with 70 members. The family could be divided into two major groups, the monocupins and the bicupins, and with 45 members, the monocupins are the larger group. The monocupins are comprised of germins and GLPs while the bicupins are predicted to be seed storage proteins (Dunwell et al. 2004). Our study focused on the OXO genes that belong to a subclass of the monocupins and are predicted to be involved in disease defense responses.
Excluding the OXLP cluster, which were more distinct, there are five monocupin subclasses (>50% nucleotide and AA identity). Most of the members of the subclasses occur in tandem clusters in the same genomic loci, suggesting that these arose from tandem gene duplications, where a sequence of DNA is transferred to create two adjacent copies (Elemento et al. 2002). This is consistent with observations for Arabidopsis, where the level of tandem gene duplication in the germin family is more than two standard deviations above the genome median (Cannon et al. 2004). Our analyses show that the OXO mapped to a QTL in chromosome 3 associated with resistance to rice blast (Ramalingam et al. 2003; Wu et al. 2004) contains four tandemly duplicated OXOs in a 10 kb region. While the gene-coding region of the four OXO genes have >90% nucleotide identity, analyses of their 1,000-bp upstream regions within Nipponbare show little nucleotide identity, which may affect how duplicate genes function in nature. Gene duplication has been predicted to create gene diversity because the duplicated genes can diverge to acquire new functions (Ohno 1970; Roth et al. 2006). However, most eukaryotic genome studies show that the sequence of the duplicated genes do not diverge as much as expected (Force et al. 1999; Lynch et al. 2001). The duplication–degeneration–complementation model of gene duplication (Force et al. 1999) predicts that degenerative mutations in regulatory elements can increase (rather than reduce) the probability of duplicate gene preservation and predicts that the usual mechanism of duplicate gene preservation is the partitioning of ancestral functions rather than the evolution of new functions. This is true for the rice monocupin family, where the duplicated genes retain relatively high nucleotide identity.
A survey of the expression of monocupins in EST libraries (http://www.tigr.org/tdb/e2k1/osa1/locus_expression_evidence.shtml) indicates a very wide range of expression patterns and indicates that many gene family members are functional. By correlating available EST data with gene structure, only genes predicted to have both 5’ and 3’ UTRs are expressed, although some members with only one UTR have ESTs available. For the rice monocupins, subfamilies have members both with and without both UTRs, and thus it appears that there are members of each subfamily that may be expressed at low levels or not at all.
Only OsOXO4 was expressed in cvs. Vandana, Moroberekan or progenies between the two cultivars in the M. oryzae interaction. OsOXO4 was induced in both resistant and susceptible interactions, but the expression of OsOXO4 occurred 24 h earlier in the resistant interaction. In QTL-based defense response, it is common for resistance mechanisms to be expressed both in resistant and susceptible interactions with expression occurring sooner and more strongly with resistance (Bell et al. 1986; Chittoor et al. 1997; Steiner-Lange et al. 2003). Also, OsOXO4 expression was only detected after inoculation by the pathogen, suggesting that early induction of OsOXO4 expression, rather than basal expression, is important in defence responses to blast infection. This differs from the expression of the OsGLP on chromosome 8, which exhibited basal levels of expression prior to blast inoculation (Manosalva et al. 2008).
Expression of OsOXO4 can be correlated with EST data, where it is expressed in stressed leaves, as well as with MPSS data, where it is only expressed during the interactions with the rice blast and bacterial blight pathogens. Based on the MPSS data, expression of OsOXO4 was lower in the M. oryzae-Nipponbare (Pi-9) resistant interaction. However, our results show that it was induced at a higher level in the M. oryzae-Moroberekan resistant interaction. The lower level of expression in the MPSS data may be due to the resistance resulting from a major gene, Pi-9, which involves a different R gene-mediated resistance pathway (Qu et al. 2006).
A comparison of the 1,000-bp upstream of the transcriptional start site of the OsOXO4 in the cultivars Moroberekan and Vandana showed 100% identity, except for a 26-bp region that was not present in Vandana. In this region, four copies of the cis-elements related to bacterial nodulation responsive genes are found. This cis-element is similar to the VfLb29 promoter that was found to be specifically active not only in the infected cells of the nitrogen-fixing zone of root nodules but also in arbuscule-containing cells of transgenic Vicia faba and M. truncatula roots colonized by the endomycorrhizal fungus, Glomus intraradices (Vieweg et al. 2004). In Rhizobium–legume interactions, Nod-factor-induced changes in ion fluxes across membranes, followed by establishment of an oscillation of intracellular Ca2+ concentration, point to the involvement of a receptor-mediated signal transduction pathway (Downie and Walker 1999). This is similar to the oxidative burst involving OXO, where there is also a flux in intracellular Ca2+ concentration in the degradation of Ca-oxalate by OXO (Lamb and Dixon 1997). While both the susceptible and resistant rice varieties have the bacterial nodulation responsive cis-element in their promoters, the additional copies in Moroberekan and the resistant progenies may be responsible for the earlier expression of OsOXO4 compared to Vandana and the susceptible progeny. Similarly, in X. oryzae pv. oryzae–rice interaction, a slight difference in the promoter sequence of the resistance gene Xa27 also affected gene expression and disease resistance. For Xa27, the absence of a 10- and 25-bp region in the promoter of the allele from the resistant line was associated with resistance expression following inoculation with incompatible X. oryzae pv. oryzae strain (Gu et al. 2005).
The ratio of synonymous to nonsynonymous substitution was also examined among the monocupin subclasses. It is a measure of selection by comparing the rate of synonymous amino acid replacement with the rate of nonsynonymous amino acid replacements (Yang and Nielsen 2000). Among monocupins, this analysis suggests that the OXO and the OXLP subclasses are under purifying selection. Given the role of OXOs and OXLPs in disease resistance (Zhang et al. 1995; Hurkman and Tanaka 1996; Faris et al. 1999; Schweizer et al. 1999; Liu et al. 2004; Wu et al. 2004; Federico et al. 2006), one might have expected that those genes would all be under diversifying selection consistent with that of the NBS-LRR and other resistance (R) gene families (Richly et al. 2002; Meyers et al. 2003; Monosi et al. 2004; Fritz-Laylin et al. 2005; Mondragon-Palomino and Gaut 2005). However, R genes function in pathogen recognition and signal transduction to induce the HR, whereas OXOs and OXLPs are downstream of the signal transduction pathway for defense response (Tenhaken et al. 1995; Zhou et al. 1998) and may have different selection pressure than R genes. The barley GLPs, which have a role in defense response as well as plant development, are also under purifying selection (Federico et al. 2006). Thus, the purifying selection for the OXO and the OXLP subclasses could be consistent with the need for a particular structure/function in the defense response that favors preservation of protein function over coevolution with the pathogen.
Considerable evidence for the association of OXOs to resistance to fungal pathogens is available (Thompson et al. 1955; Zhou et al. 1998; Berna and Bernier 1999; Hu et al. 2003; Ramalingam et al. 2003; Livingstone et al. 2005). The barley HvOXO gene is expressed in the mesophyll tissue and the activity of the OXO enzyme increases in leaf extracts in response to Bgh, which may be a source of H2O2 (Zhou et al. 1998). Transgenic sunflower and peanuts transformed with wheat OXO and HvOXO, respectively, exhibited enhanced resistance to Sclerotinia species (Hu et al. 2003; Livingstone et al. 2005). OXOs were also mapped to QTLs involved in fungal resistance in wheat and rice (Faris et al. 1999; Ramalingam et al. 2003; Friesen and Faris 2004; Wu et al. 2004). The results of this study further support the role of specific OXO genes in resistance. The presence of OXOs only in cereals provides an interesting speculation into the evolutionary process in which members of the cupin superfamily diversified after the divergence of monocots and dicots 200 millions years ago (Wolfe et al. 1989). It is probably likely that specialization into OXOs occurred after the divergence.
Our study presents the rice OXOs as members of the cupin superfamily of proteins and highlights how analysis of gene structure can help identify candidates for contribution to disease resistance. OXOs are a unique group of proteins in cereals with converging evidences for their roles in defense response. We show that the OXOs form a cluster of four tandemly duplicated copies co-localizing with chromosome 3 QTL associated with resistance to rice blast. Specifically, we have shown that among the four tandemly duplicated OsOXOs, only OsOXO4 is expressed during M. oryzae–rice interaction, and it is expressed earlier during resistance than susceptibility. A unique feature of the resistance-associated OsOXO4 is that the resistance-associated sequence difference is not in the coding region but in a 26-bp sequence in the promoter region containing additional cis-elements associated with bacterial responsive nodulation genes. These cis-elements may also play a role in pathogen response. It would be interesting to know if transferring these cis-elements to the promoter of OsOXO4 in a susceptible cultivar, like Vandana, would also induce expression of OsOXO4 at an earlier time point and provide increased resistance.
Retrieval of cupin sequences and characterization of monocupin genes in the rice genome
To identify the complete set of proteins in the rice genome similar to OXO, we designed a PERL script to retrieve proteins with the cupin motif [G(X)5HXH-(X)3,4E(X)6G] and G(X)5PXG(X)2H(X)3N] from build 4 of the O. sativa ‘Nipponbare’ genome sequence obtained from the TIGR Rice Genome Annotation database (http://tigr.org/tdb/e2k1/osa1) (Yuan et al. 2005). Protein prediction was done using FGENESH (http://linux1.softberry.com/berry.phtml). ClustalX (Thompson et al. 1997) was used to align the protein sequences and generate phylogenetic trees. A total of 1,000 bootstrap replicates were performed using neighbor-joining, and clades having >60% bootstrap support were identified (Fritz-Laylin et al. 2005).
From the collection of cupin sequences, each sequence was classified into monocupin or bicupin using the Conserved Domain-Search option of GenBank’s Conserved Domain Database (Marchler-Bauer et al. 2005). The sequences were annotated by conducting BLAST search against the Swiss-Prot database for curated sequences (Schneider et al. 2004). The physical location of each sequence as well as the gene structure prediction were obtained from the TIGR Rice Annotation database and visualized by GenomePixelizer (Kozik et al. 2002).
ProtParam in Expasy (http://www.expassy.ch) was used to analyze the monocupin protein characteristics by determining amino acid length, isoelectric point stability index as well as hydrophobicity using Kyte-Doolitle method. To determine cellular localization, pSORT was used (http://wolfpsort.seq.cbrc.jp/). SignalP (http://www.cbs.dtu.dk/services/SignalP/) was employed to predict N-terminal signal peptides and MEME (Bailey and Gribskov 1998) was used to identify conserved protein motifs in the sequences. Selection pressure among monocupins was determined using codeml from the PAML package (Yang 1997; Yang and Nielsen 2000). A ω > 1 indicates diversifying selection while a ω < 1 indicates purifying selection. Of the six subclasses, only rhicadhesin-like receptor proteins, OXO and OXLP meet the criteria for calculating ω which are based on the following: (a) >50% similarity at the amino acid level, (b) absence of long insertions and deletions, and (c) at least three members. Evidence for monocupin gene expression was derived from the Rice Gene Expression Evidence page from TIGR (http://www.tigr.org/tdb/e2k1/osa1/locus_expression_evidence.shtml) and KOME (http://cdna01.dna.affrc.go.jp/cDNA/). The gene structure and orientation of monocupins were derived from the TIGR Rice Genome database build 5.0.
Expression of chromosome 3 OXOs
Plant materials used were O. sativa cvs. Moroberekan and Vandana and four advanced backcross progenies from a Vandana × Moroberekan cross (Wu et al. 2004). These materials were screened for resistance to rice blast under field conditions in India and the Philippines (Carrillo, Variar, Bhatt and Vera Cruz, unpublished). Molecular analysis of the progenies, IR78221-19-6-7-B, IR78221-19-6-56-B, and IR78221-19-6-99-B, show that they contain the QTL allele for OXO in chromosome 3 from resistant donor parent, Moroberekan, while the progeny, IR78221-19-3-196-B, contains the OXO allele from the susceptible parent, Vandana.
RT-PCR was used to detect the expression patterns of the OXO genes present on chromosome 3. Fifteen 14-day-old seedlings of each plant were inoculated with M. oryzae strain PO6-6 (100,000 spores/ml). Total RNA was extracted from leaves sampled at 0-, 6-, 12-, 24-, and 48-h post-inoculation using Trizol (Invitrogen, Carlsbad, CA, USA) and treated with DNAse (Promega, Madison, WI, USA). Two micrograms of RNA was used for first strand cDNA synthesis in a 20 µL reaction volume with Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s recommendations with oligodT(20) primer. Each cDNA was amplified using gene-specific OXO primer pair (10 μM) using 30 cycles of PCR amplification. Amplification of the actin gene, LOC_Os08g17820, was used as positive control for each gene. We designed gene-specific primers with the following sequences (5’–3’): (a) LOC_Os03g48750 (OsOXO1) F: ACCCTGACCCTCTCCAAGAC, R: CGGACCACCCTGGAGTAGTA; (b) LOC_Os03g48760 (OsOXO2) F: GGACCCTCTCCAGGACTTCT, R: CACGGACCACCTTGGAATAG; (c) LOC_Os03g48770 (OsOXO3) F: GCCTCTACGGGGTCTTGACT, R: CACGGACCACCTTGGAGTAG; (d) LOC_Os03g48780 (OsOXO4) F: AGCTTGTCACTGCGCTTCTT, R: GTGGCAATCTTGGAGGAGAA; and (e) Actin F: GTGGAGAGGCTGTGGAAGG, R: ATGTTCAGGGCTTTGCC.
In addition, the rice gene expression data from different expression libraries were also collected from the DCFI Rice Gene Index (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=Rice). The Rice Gene Index uses a highly refined protocol to analyze EST sequences in an attempt to identify the genes represented by that data and to provide additional information regarding those genes (Quackenbush et al. 2001). MPSS signatures for each OsOXO were derived from the Rice MPSS database (http://mpss.udel.edu/rice/). The Rice MPSS database is a repository of rice gene expression data derived from a sequencing-based technology that uses a unique method to quantify gene expression level, generating millions of short sequence tags per library (Meyers et al. 2004b; Nakano et al. 2006).
DNA isolation and sequencing
Leaves from 2-week-old greenhouse-grown plants were harvested for DNA extraction using the CTAB method. Gene-specific primers to amplify the four OXO genes in chromosome 3 were designed using the PRIMER3 program (Rozen and Skaletsky 2000). Three pairs of overlapping primers for sequencing the genes were designed that would encompass the 1-kb upstream region, the gene coding region and the 3’UTR region of each gene (as shown in Supplementary Table 5). The expected product sizes were 800–1,000 bp. DNA amplifications were performed in a 50-μL volume. The reactions contained 100 ng of genomic DNA, 0.25 mM of each primer, 2 mM of each dNTP, 1.50 U Taq DNA polymerase (New England Biolabs, Cambridge, MA, USA), and 1× PCR buffer (New England Biolabs, Cambridge, MA, USA). The amplification profile is as follows: 94°C for 5 min, 35 cycles of 1 min at 94°C, 1 min at 55°C, 2 min at 72°C, and followed by a final extension of 7 min at 72°C. PCR products were analyzed on agarose gel, purified using a Qiaquick PCR purification kit (Qiagen, Valencia, CA, USA), and quantitated prior to DNA sequencing. PCR products were sent to Macrogen (Seoul, Korea) for sequencing.
We gratefully acknowledge the financial support from the Swiss Agency for Development and Cooperation and the Generation Challenge Program. We thank Kenneth McNally, Richard Bruskiewich, and Jun Ulat for advice on bioinformatics and Rebecca Davidson and Teresa de Leon for helpful discussions.
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