High Resolution Mapping of QTLs for Heat Tolerance in Rice Using a 5K SNP Array
© The Author(s). 2017
Received: 29 October 2016
Accepted: 30 May 2017
Published: 5 June 2017
Heat stress is one of the major abiotic threats to rice production, next to drought and salinity stress. Incidence of heat stress at reproductive phase of the crop results in abnormal pollination leading to floret sterility, low seed set and poor grain quality. Identification of QTLs and causal genes for heat stress tolerance at flowering will facilitate breeding for improved heat tolerance in rice. In the present study, we used 272 F8 recombinant inbred lines derived from a cross between Nagina22, a well-known heat tolerant Aus cultivar and IR64, a heat sensitive popular Indica rice variety to map the QTLs for heat tolerance.
To enable precise phenotyping for heat stress tolerance, we used a controlled phenotyping facility available at ICAR-Indian Institute of Wheat and Barley Research, Karnal, India. Based on ‘days to 50% flowering’ data of the RILs, we followed staggered sowing to synchronize flowering to impose heat stress at uniform stage. Using the Illumina infinium 5K SNP array for genotyping the parents and the RILs, and stress susceptibility and stress tolerance indices (SSI and STI) of percent spikelet sterility and yield per plant (g), we identified five QTLs on chromosomes 3, 5, 9 and 12. The identified QTLs explained phenotypic variation in the range of 6.27 to 21. 29%. Of these five QTLs, two high effect QTLs, one novel (qSTIPSS9.1) and one known (qSTIY5.1/qSSIY5.2), were mapped in less than 400 Kbp genomic regions, comprising of 65 and 54 genes, respectively.
The present study identified two major QTLs for heat tolerance in rice in narrow physical intervals, which can be employed for crop improvement by marker assisted selection (MAS) after development of suitable scorable markers for breeding of high yielding heat tolerant rice varieties. This is the first report of a major QTL for heat tolerance on chromosome 9 of rice. Further, a known QTL for heat tolerance on chromosome 5 was narrowed down from 23 Mb to 331 Kbp in this study.
Rice is a major staple food crop for nearly half of the world population. The global population is projected to grow from seven to nine billion by 2050 and to reach ten billion before 2100 (United Nations 2011). To ensure food security to the added population, rice production has to increase by 0.6 to 0.9% annually until 2050 (Carriger and Vallee 2007). However, rise in global average temperature to the tune of 0.5 °C in the twentieth century and future projections in the range of 1.4–5.8 °C by the end of this century (IPCC 2007), will be detrimental to crop yield (Lobell et al. 2011). Declining farmland resources coupled with global warming have forced rice cultivation to marginal environments and beyond the normal rice season. This in turn has exposed the rice crop to higher day temperature (>33 °C) adversely impacting seed set (Nakagawa et al. 2002; Prasad et al. 2006; Jagadish et al. 2010a).
Heat stress alters the initiation and duration of developmental phases, especially the duration from floral/panicle initiation to anthesis/panicle exertion in plants (Sato et al. 1973). Heat stress during flowering and anthesis can lead to failure in fertilization due to pollen or ovule sterility (Matsui et al. 1997). Early reproductive processes viz., micro- and megasporogenesis, pollen and stigma viability, anthesis, pollination, pollen tube growth, fertilization, and early embryo development are all highly susceptible to heat stress (Giorno et al. 2013). Flowering stage is crucial for crop productivity as heat stress during this phase causes reduced pollen fertility and low seed set in rice (Jagadish et al. 2010a). Anthesis processes, including anther dehiscence, pollination, and pollen germination are most sensitive to high temperature stress in rice. The main cause of spikelet sterility induced by high temperature is anther indehiscence (Matsui et al. 1999). High temperature inhibits swelling of pollen grains, which is a driving force for anther dehiscence in rice. Successful anther dehiscence depends on several parameters, including rupturing of septa, expansion of locule walls, pollen swelling, and rupturing of stomium (Liu et al. 2006).
Enhanced heat tolerance in rice is required at flowering stage to avoid spikelet sterility. Since 1985, germplasm screening for high temperature stress tolerance has been carried out by different research groups worldwide (Sarwar and Avesi 1985; Matsui and Omasa 2002; Jagadish et al. 2007, 2008). Heat tolerance at flowering stage in rice is attributed to multiple genes with cumulative effects on trait expression, otherwise called as quantitative trait loci (QTL; Cao et al. 2003; Xiao et al. 2011a; Ye et al. 2012). The discovery of genes/QTLs for enhanced tolerance to high temperature stress has practical implications in agriculture. Mapping of QTLs for heat tolerance in rice was first reported by Cao et al. (2003) based on percent spikelet fertility using doubled haploid population derived from IR64/Azucena cross. Thereafter many research groups have mapped QTLs for heat stress tolerance using F2, back cross inbred lines (BIL) and recombinant inbred lines (RIL) populations, evaluated at the time of heading in controlled environment conditions (Chang-Lan et al. 2005; Chen et al. 2008; Zhang et al. 2008, 2009; Jagadish et al. 2010b; Xiao et al. 2011a; Ye et al. 2012, 2015; Cheng et al. 2012; and Poli et al. 2013). Some of these studies created high temperature condition for phenotyping by late planting in open field (Xiao et al. 2011b; Tazib et al. 2015; and Zhao et al. 2016). Almost all of these studies employed RFLP or SSR markers, except Ye et al. (2012, 2015), who used 300 SNP markers for the QTL mapping. IR64 has been used as one of the parental lines in generating mapping populations for mapping heat stress tolerance QTLs in some studies (Cao et al. 2003; Ye et al. 2012, 2015), while Nagina22 and its derived mutant lines have been used as parents in generating mapping population by other researchers (Buu et al. 2014; Poli et al. 2013). There is a report on using IR64/Nagina22 derived F2 population for mapping heat tolerance QTLs (Ye et al. 2012).
Mapping of QTLs for heat stress tolerance using stress tolerance indices, which compare the performance of genotypes under control and stress condition, have not been reported earlier, but it has been utilized for mapping salt stress tolerance (Fernandez 1992; Pandit et al. 2010; Tiwari et al. 2016). The relative performance of genotypes under stress and control conditions can be used as an indicator to identify and map QTLs, which can be further used in breeding crop varieties for stress tolerance, rather than mapping QTLs based on phenotypic performance in stress environment alone (Raman et al. 2012). This has practical relevance since genotypes with low yield potential under control condition quite often show higher tolerance to stress than high yielding genotypes. Genomic regions governing salinity stress tolerance was successfully mapped in rice using stress indices (Pandit et al. 2010, Kumar et al. 2015; Tiwari et al. 2016). The present study focused on identification of QTLs for heat stress tolerance at flowering stage in a RIL mapping population derived from Nagina22/IR64 cross using controlled phenotyping facility for imposing heat stress, using stress tolerance indices for normalization of intrinsic differences in yield potential and high density SNP mapping. High density linkage map is expected to result in finding QTLs flanked by closely linked markers that can be readily used in breeding programmes for marker assisted selection.
We used 272 F7:8 RILs developed through single seed descent method from a cross between Nagina22 (N22), a heat tolerant cultivar (Mohapatra et al. 2014; Prakash et al. 2016) and IR64, a heat susceptible cultivar (Jagadish et al. 2010). To achieve synchronized flowering, the RILs were first phenotyped for days to 50% flowering and grouped into three categories as early, medium and late flowering types and then were sown in a staggered manner for synchronization of their flowering time. This exercise enabled us to impose heat treatment at a uniform stage in the population that in turn minimized the interference of phenological differences in analysis.
Heat Stress Treatment
Phenotyping of the F8 RILs for Heat Stress Tolerance
Five individual plants from each RIL were sampled and harvested separately. Main panicle from each plant was used for analysing spikelet fertility by counting the number of filled and empty spikelets. The remaining panicles were collected separately from each plant and utilised for calculating yield potential of RILs under heat stress. In the same way, five plants from each RIL were harvested from the control experiment for evaluating the performance of genotypes under ambient conditions, used for computing their stress response index. The response of genotypes to heat stress was expressed as stress susceptibility index (SSI) given by Fisher and Mauer (1978) and stress tolerant index (STI) given by Fernandez (1992). SSI assesses the reduction in yield caused by stress as compared to favourable environment. The heat tolerant genotypes would have lower SSI value, which indicates lower difference in their yield across control and stress while it would be vice-versa for the susceptible genotypes. Thus, SSI helps to identify more tolerant lines. STI, on the other hand, helps to identify genotypes that produce higher yield in control as well as stress conditions, which is more desirable for practical reasons. The tolerant lines have higher STI value.
Genotyping of RILs
Genomic DNA of all RILs, and the two parents was isolated from pooled young green leaves from plants grown in a row in the field by CTAB method (Doyle and Doyle 1990). DNA was quantified using NanoDrop 8000 spectrophotometer (Thermo Scientific, USA) and concentration of DNA was adjusted to a minimum of 50 ng/μl and approximately 200 ng of DNA from each genotypes were used for hybridization in Illumina Infinium® II genotyping assay. A customized array with 5246 SNPs in abiotic stress responsive genes of rice employing Illumina Infinium® II design probes and dual color channel assays (Infinium HD Assay Ultra, Illumina), was used for genotyping, following the manufacturer’s protocol (Kumar et al. 2015).
SNP Genotype Calling
SNP genotyping data obtained from the array were analysed using Genome Studio V2010.1 (Illumina Inc.). SNPs were called using genotyping module integrated in the software where individual SNP is viewed as GenoPlots. Data quality was confirmed with internal controls and QC functions such as GenTrain and GenCall scores. After calling the data automatically, the SNPs were re-scored and checked for their presence in a canonical cluster to get a GenTrain score > 0.7. The samples with call rates of <0.89 and the SNPs with norm R values <0.2 were removed from further analysis.The genotype calls from parental lines Nagina22 and IR64 were converted into AA and BB, respectively and similarly SNP calls of segregating genotypes were transformed in concordance with either parental type and used for the construction of framework linkage map.
QTL Mapping and Epistatic Interaction Network
QTL mapping was carried out using QTL IciMapping software v4.0 (Meng et al. 2015). Segregation pattern of each SNP in the RIL population was analysed using chi-square test with statistical significance at P value of 0.01. The redundant markers with identical scores were removed since they cannot provide any additional information. Markers with correlation coefficient of 1 were deleted by choosing missing proportion option. The genetic distance (cM) between SNP markers was converted to physical distance (kb) with 1 cM equal to 260 kbp (Chen et al. 2002; Tiwari et al. 2016). QTLs for heat stress tolerance were mapped using BIP functionality available in the QTL IciMapping software. Inclusive composite interval mapping of additive and dominant QTL (ICIM-ADD) mapping method was chosen along with the following parameters viz., window size of 1 cM, 500 permutations and type I error at 0.05 to call for QTL. The LOD threshold was set at 3.5 to accept the call as a significant QTL. The epistatic interaction network was determined for all the four traits studied using MQM algorithm in R/QTL (Browman et al. 2003).
In-silico Identification of Non-synonymous SNPs in Genes Present in the Mapped QTL Intervals
Non-synonymous SNPs between N22 and IR64 were identified using Rice SNP-Seek Database (Mansueto et al. 2017) with Nipponbare as reference genome and N22 and IR64 as query genomes. The gene locus id was given as input to retrieve the non-synonymous SNPs between N22 and IR64 for further analysis.
Phenotypic Variation and Correlation Among Traits in the RIL Population
Performance of the parents and their recombinant inbred lines under control and heat stress
Percent spikelet sterility
Yield per plant (g)
Phenotypic variation for heat stress tolerance indices in Nagina22/IR 64 RIL mapping population
SSI for % spikelet sterility
STI for % spikeletsterility
STI for yield per plant
SSI for yield per plant
SNP Marker Segregation and Framework Linkage Map
Selection of polymorphic markers for QTL mapping
No. of Markers after Chi square test at 0.01 significant level
% Segregation distortion
No. of markers per chromosome after removing redundant markers
% Redundant markers
No. of markers used for QTL analysis
QTLs for Heat Tolerance Traits
QTLs for heat stress indices identified in RIL mapping population derived from N22 and IR64
Physical position (Mb)
STI for % spikelet sterility
SSI_ for % spikelet sterility
STI for yield per plant
SSI for yield per plant
SSI for yield per plant
Candidate Genes for Heat Tolerance Located in the QTL Intervals
The genes located in the four genomic regions for the identified QTLs were extracted (Additional file 2 : Table S1). Probable candidate genes for heat tolerance located in the major QTL interval qSTIPSS9.1 for spikelet fertility were PTC1, glycosyltransferase, microtubule associated protein, annexin and HSFs. Similarly, candidate genes for heat tolerance index in the narrowed down QTL interval qSTIY5.1/qSSIY5.2 included, trehalose synthase, trehalose-6-phosphate synthase, auxin response factor and calcineurin B-like protein-interacting protein kinase (CIPKs). In the QTL interval qSSIPSS12.1, 134 genes were identified including lipases, laccase, isoflavonereductase, cyclopropane-fatty-acyl-phospholipid synthase, OGR1, wall associated receptor kinase and pentatricopeptide protein coding genes. The qSSIY3.1 QTL harboured 142 genes including, TFs, signalling genes and floral organ developmental genes, tesmin/TSO1, Crinkly4 receptor-like kinase, LIM domain containing protein gene and pectin methylesterase inhibitor coding gene. The majority of these genes are reported to be involved in pollen grain development, pollen tube growth and fertilization in rice.
Digenic Interactions of QTLs for Heat Tolerance
Epistatic interaction network
Physical position (bp)
WD domain, G-beta repeat domain containing protein, expressed
dehydrogenase, putative, expressed
Non-synonymous SNPs in the mapped QTL intervals
Non-synonymous SNPs between N22 and IR64 in the mapped QTL region for heat stress tolerance
No. of genes with SNPs
No. of SNPs
Non-synonymous SNPs between N22 and IR64
qstiy5.1 & qssiy5.2
QTLs for heat tolerance have been mapped on different chromosomes of rice by different research groups during the last decade (Cao et al. 2003; Chen et al. 2008; Zhang et al. 2008, 2009; Jagadish et al. 2010; Xiao et al. 2011; Ye et al. 2012, 2015) (Additional file 2: Table S3). In the current study, using a reasonably large RIL population, high density SNP map and phenotyping under controlled facility, we identified four heat tolerant QTLs in rice, of which three were novel namely qSTIPSS9.1, qSSIPSS12.1 and qSSIY3.1. Among these, qSTIPSS9.1 was the major QTL for percent spikelet sterility. Further, we also identified a known major effect QTL, qSSIY5.1/qSTIY5.1 for both the indices of yield. Zhang et al. (2008) have reported this QTL in a RIL mapping population derived from a cross Zhongyouzao8 x Toyonishiki between SSR markers, RM405 and RM274 flanking a 23 Mb interval. In their study, this QTL explained 10.7% phenotypic variation for spikelet fertility under heat stress while it was for SSI/STI for yield in our study. Further, the QTL interval was narrowed down to a 331 kbp region comprising of 54 genes in our study. This was because earlier studies have used either SSR markers (maximum 264) or less than 300 SNP markers for mapping QTLs for heat tolerance whereas we have used more than 800 SNPs and 272 RILs to achieve a much higher resolution (Buu et al. 2014; Ye et al. 2012, 2015). Our ability to identify QTLs in such narrow intervals could be attributed to the use of 5K SNP array comprising of SNPs from abiotic stress responsive genes (Kumar et al. 2015). Some important candidate genes located in the high effect and minor QTLs identified in the present study are discussed below for their probable role in enhanced spikelet fertility and yield under heat stress.
There were 65 genes in the QTL region, qSTIPSS9.1, including transporters, transcription factors such as HSF (OsHsfB4c), PHD-finger domain containing TF (PTC1), bHLH, and C2H2 zinc finger, transcriptional regulators, glycosyltransferase microtubule associated protein, and annexin (Additional file 2: Table S1). Tapetum, the innermost cell layer of the anther wall, plays a crucial role in anther development, microspore/pollen formation, and pollen wall formation. During late pollen development, tapetal degeneration triggered by an apoptosis-like process is essential for viable pollen formation (Li et al. 2006). PERSISTENT TAPETAL CELL1 (PTC1) present in the qSTIPSS9.1 QTL region encodes a PHD-finger protein that controls programmed tapetal development and degradation to ensure functional pollen formation in rice (Li et al. 2011a, b). PTC1 is expressed specifically in tapetal cells and microspores during anther development in stages 8 and 9 and initiates a typical apoptosis-like cell death, thereby ensuring proper pollen grain development (Li et al. 2011). Loss of function of PTC1 displayed uncontrolled tapetal cell proliferation and swelling, delayed DNA fragmentation, and pollen wall development, causing complete male sterility (Li et al. 2011). Timely initiation of tapetal programmed cell death is essential for the regulated release of wall materials from the tapetum to the developing microspore including carbohydrate, lipid molecules, and other nutrients. This gene might be responsible for maintaining higher fertility in N22 under heat stress by timely initiation of PCD in N22 tapetal cell to ensure more fertile pollen grains than in the susceptible parent IR64. This gene otherwise named as a tms9–1/OsMS1 is responsible for thermo-sensitive genic male sterility in HengnongS-1, one of the oldest and often-used TGMS line in indica two-line hybrid rice breeding programs in China (Qi et al. 2014). Also, Arabidopsis thaliana MALE STERILITY1 (MS1) gene encodes for a protein homologous to the PHD-finger class of transcription factor and has been demonstrated to be involved in tapetal development and pollen wall biosynthesis (Yang et al. 2007).
Glycosyltransferase attaches a single or multiple sugars to different bio-molecules and highly expresses in mature pollen grains and is involved in mature pollen grain formation in rice (Moon et al. 2013). GLYCOSYLTRANSFERASE1 (OsGT1) of rice present in qSTIPSS9.1 is involved in pollen wall formation, especially, the exine and intine construction and pollen maturation. The osgt1 mutant failed to produce mature pollen grains since its pollen had disrupted intine structure owing to low levels of starch and protein (Moon et al. 2013). Similarly, uneven pattern of exine 1 (upex1) gene of Arabidopsis encodes GT31 family glycosyltransferase in Arabidopsis and might be involved in galactosylation of arabinogalactan proteins (AGPs). The mutant of UPEX1 exhibit defective and irregular exine pattern and suggests that primexine localized AGPs could play a role in sporopollenin adhesion and patterning in early microspore wall development (Li et al. 2016). HSFs are main players in imparting heat stress response by activating transcription of downstream genes including HSPs (Guo et al. 2008). AtHsfB4 has a role in root development in Arabidopsis and involved in early stage of heat shock (Lohmann et al. 2004; Begum et al. 2013). A similar OsHsf has been identified in this major QTL on chromosome 9, which is yet to be characterized in rice.
Microtubule-associated proteins play a crucial role in the regulation of microtubule dynamics, and important for plant cell and organ development (Liu et al. 2013). The 65-kD microtubule-associated protein (MAP65) family member in Arabidopsis (AtMAP65–1) is ubiquitously expressed during the cell cycle and in all plant organs and tissues with the exception of anthers and petals (Smertenko et al. 2004). However, Microtubule-associated protein MAP65–1a (LOC_Os09g27700) of rice is expressed in anther and pistil. This might be indicative of its role in reproductive organ development in rice and hence is a good candidate for further studies in rice. Annexin functions to counteract oxidative stress, maintain cell redox homeostasis, and enhance drought tolerance (Szalonek 2015). Down-regulation of Arabidopsis annexin5 (Ann5) in transgenic Ann5-RNAi lines caused sterile pollen grains. Ann5 is involved in pollen grain development, germination and pollen tube growth through the promotion of endo-membrane trafficking modulated by calcium (Zhu et al. 2014). TaAnn10 in wheat is highly expressed in floral bracts, pistil, anthers and immature endosperm and it correlates with anther development. But it fails to be induced by low temperature in thermosensitive genic male sterile lines, suggesting that specific down-regulation of TaAnn10 is associated with cold induced male sterility in wheat (Xu et al. 2016). The relative expression levels of TaAnn10 in the stamen strongly correlated with male fertility in recovery lines (Xu et al. 2016). One such annexin 10 (OsANN10/ LOC_Os09g27990) is present in the QTL interval qSTIPSS9.1.
A common response of organisms to drought, salinity, and temperature stresses is the accumulation of sugars and compatible solutes including trehalose. The increased trehalose accumulation correlates with elevated capacity for photosynthesis under both stress and non-stress conditions in rice (Garg et al. 2002). Trehalose-6-phosphate synthase (TPS) plays an important role in trehalose metabolism and signalling. Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances the tolerance of rice seedling to cold, high salinity and drought stress without other significant phenotypic changes (Li et al. 2011). Similarly, the over-expression of trehalose-6-phosphate phosphatase in maize ears increases both kernel set and harvest index in drought stress condition. Increase in yield to the tune of 9% to 49% under non-drought or mild-drought conditions, and 31% -123% under more severe drought conditions, relative to yields from non-transgenic controls was observed (Nuccio et al. 2015). Similarly, trehalose concentration increased upon 4 h of heat stress at 40 °C and 4 days after cold stress at 4 °C in Arabidopsis thaliana (Kaplan et al. 2004). Over-expression of ScTPS1 and ScTPS2 under stress associated rd29A promoter provided protection against drought, salt, freezing, and heat stress (Miranda et al. 2007). Hence, trehalose synthase and trehalose phosphate synthase are a probable candidate genes underlying QTL qSSIY5.1/qSTIY5.1 for yield under heat stress.
Our analysis for non-synonymous SNPs between the parents in the candidate genes like PTC1, tms9–1/OsMS1, OsGT1,MAP65–1a (LOC_Os09g27700), OsANN10, trehalose synthase, trehalose phosphate synthase, OsCR4, pectin methyl esterase and tesmin could not find allelic variants. This could be due to low coverage of the sequence data in either or both of the parents or lack of variation present in coding regions in the above genes. Further, the InDel polymorphism for these genes is not known. Alternatively, there could be variations in promoter, intron-exon junctions and UTR regions, which are not yet known. Hence, future effort is required to deep sequence these regions in the parents to identify polymorphisms, if any, in candidate genes. Nevertheless, the listed SNPs between N22 and IR64 can be utilised for fine mapping and functional validation of the QTLs.
The interaction network analysis showed evidence for the involvement of the major QTL, qSSIY5.1/qSTIY5.1, in digenic interactions, strengthening the role of this region in imparting heat tolerance. Though most of the SNPs involved in epistasis were in genes encoding uncharacterized expressed proteins, two SNPs were from known proteins coding genes namely, WD domain, G-beta repeat domain containing gene (LOC_Os05g44320) and dehydrogenase gene (LOC_Os04g52280). The WD40 protein is reported to play a role in diverse protein-protein interactions or protein-DNA interactions by acting as scaffolding molecule and promoting protein activity and thus functioning as a positive regulator of plant responses to various abiotic stresses such as salinity, osmotic and dehydration stress in plants (Mishra et al. 2012; Kong et al. 2015). Further, it is involved in various biological process, viz., signal transduction, gene transcriptional regulation, protein modifications, cytoskeleton assembly, vesicular trafficking, DNA damage and repair, cell death and cell cycle progression (Zhang and Zhang 2015).
Present study using Nagina22/IR64 RIL mapping population and a 5K SNP genotyping chip, identified a major novel QTL qSTIPSS9.1 for reproductive stage heat tolerance in a 394 kbp region of rice chromosome 9. The study also confirmed the presence of a known major QTL for heat tolerance on chromosome 5 (qSTIY5.1/qSSIY5.1), which was narrowed down from 23 Mb in the original study to a much smaller interval of 331 kbp. This QTL was also involved in digenic interaction. Though the polymorphism survey in the candidate genes using the available data did not produce any trait linked variation, the SNPs identified could be useful in fine mapping. Further sequencing and functional validation is required for the identification of actual genes in these QTL regions responsible for the heat tolerance. Nonetheless, the two major QTLs identified here can be employed directly for crop improvement by marker assisted selection (MAS) after development of suitable scorable markers for breeding of high yielding heat tolerant rice varieties.
The first author is grateful to the Department of Science and Technology, New Delhi, India for providing financial assistance in the form of INSPIRE-fellowship. The research work was funded by National Agricultural Innovation Program (NAIP) under the project “Bioprospecting of genes and allele mining for abiotic stress tolerance”.
The research material developed by TM was advanced and maintained by PSS, CP and SVAM. Phenotyping was carried out by PSS and MKR. Genotyping was carried out by SVAM. Marker analysis was carried out by PSS and CP. QTL mapping was carried out by PSS and SVAM. In-silico search for putative genes in QTL intervals was carried out by PSS. Epistatic interaction was found by CP. PSS compiled and interpreted the results. RT provided and maintained the phenotypic facility. PSS and SVAM drafted the manuscript. NKS and TM supervised the work. NKS edited and approved the manuscript. All the authors read and approved the manuscript.
The authors declare that they have no competing interest.
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