Mesocotyl Elongation is Essential for Seedling Emergence Under Deep-Seeding Condition in Rice
© The Author(s). 2017
Received: 23 March 2017
Accepted: 10 July 2017
Published: 14 July 2017
Direct-seeding cultivation by deep-seeding of seeds (drill seeding) is becoming popular due to the scarcity of land and labor. However, poor emergence and inadequate seedling establishment can lead to yield loss in direct-seeding cultivation by deep-sowing. In rice, mesocotyl and coleoptile are primarily responsible for seedling emergence from deeper levels of soil.
Quantitative trait loci (QTLs) for mesocotyl and coleoptile length at 5-cm seeding depth were detected using 98 backcross inbred lines from a cross between Kasalath and Nipponbare. Three QTLs qMel-1, qMel-3, and qMel-6 for mesocotyl length were identified on chromosomes 1, 3, and 6, respectively, in two independent replicates. At two QTLs, qMel-1 and qMel-3, the Kasalath alleles increased mesocotyl length, whereas Nipponbare allele increased at qMel-6. The Nipponbare alleles at two QTLs (qCol-3 and qCol-5) increased the coleoptile length. Further, seeds of 54 chromosome segment substitution lines (CSSLs) from the cross between Kasalath and Nipponbare sown at 5 cm soil depth showed a significant positive correlation between seedling emergence and mesocotyl elongation (r > 0.6, P < 0.0001), but not with coleoptile elongation (r = 0.05, P = 0.7). Seedling emergence of Nipponbare, Kasalath, and the 3 of the 54 CSSLs rapidly decreased with increasing sowing depth. Seedling emergence at seeding depths of 7 and 10 cm was faster in Kasalath and CSSL-5 that harbored the Kasalath alleles across the qMel-1 and qMel-3 regions than in the other two CSSLs that contained a single QTL and Nipponbare alleles. CSSL-5 showed the longest mesocotyl among the 3 CSSLs, but no difference in coleoptile length was observed among the 3 CSSLs at seeding depths of 7 and 10 cm.
Variation of mesocotyl elongation was found to be associated with seedling emergence at the seeding depth of 5 cm. To our knowledge, this is the first study performed using CSSLs to detect QTLs for mesocotyl or coleoptile elongation and to determine the effect of mesocotyl elongation on seedling emergence in rice. Our findings provides a foundation for developing rice cultivars that show higher seedling emergence after direct seeding by introgressing QTLs for mesocotyl elongation in rice breeding.
In Asia, two rice planting methods are used: transplanting and direct seeding. In direct seeding, seeds are sown directly in wet/puddled soil, unpuddled soil, or standing water (Kumar and Ladha 2011). In Asia, approximately 21% of the total rice area is used for direct seeding, and this is expected to increase owing to the scarcity of land, water, and labor (Pandey and Velasco 2005). Rice varieties suitable for direct seeding should include high germination ability, seedling vigor, fast root growth, early tillering ability, and lodging resistance (Lee et al. 2002; Tang 2002). Faster and uniform germination and seedling emergence resulted in more vigorous seedling growth and increasing yield in direct-seeding (Farooq et al. 2006). In direct seeding, deep seeding (drill seeding) is known to reduce damages from wildlife and improve lodging tolerance. However, when seeds are sown deep, the seedlings need to elongate their organs to ensure that the plumule reaches the soil surface. Seedling emergence is an important criterion for determining the actual yield during direct seeding cultivation of rice. The coleoptile (the protective sheath that covers the emerging shoot) and mesocotyl (the structure between the scutellar and coleoptilar nodes in an embryo) of rice are primarily responsible for the emergence of seedlings from deeper soil layers (Turner et al. 1982; Dilday et al. 1990). Seeding depth is also an important factor for dry direct-seeding rice cultivation. Seedling growth for temperate japonica cultivars was adversely affected when seeds were sown deeper than the optimum seeding depth of 2–3 cm (Lee et al. 2002). However, shallow seeding can increase the incidences of damage by birds, and plants might suffer lodging after the heading stage. Thus, obtaining information on the optimum seeding depth for direct seeding for diverse germplasm is necessary (Lee et al. 2002).
Previous studies reported that mesocotyl and coleoptile elongation are governed by many genetic and environmental factors (Takahasi 1978; Takahasi 1984). Their elongation shows significant variation across genotypes. Mesocotyl elongation is greater in indica rice than in japonica rice (Suge 1972; Takahashi 1978; Lee et al. 2012), whereas the coleoptile is longer in japonica cultivars than in indica cultivars under submerged condition (Takahashi 1978). Among japonica cultivars, upland rice generally produced shorter coleoptiles and longer mesocotyls than lowland type (Chang and Vergara 1975).
Quantitative trait loci (QTLs) associated with mesocotyl and coleoptile elongation in rice have been reported. QTLs for mesocotyl elongation were detected using various segregating populations from interspecific or intrasubspecific crosses (Katsuta-Seki et al. 1996; Cai and Morishima 2002; Cao et al. 2002; Huang et al. 2010; Lee et al. 2012). Katsuta-Seki et al. (1996) reported 3 QTLs for mesocotyl elongation on chromosomes 3, 6, and 11 by using F3 populations from the cross between 2 indica cultivars grown in glass tubes. Cai and Morishima (2002) detected 11 QTLs for mesocotyl length by using 125 recombinant inbred lines (RILs) derived from a cross between an indica cultivar and a strain of wild rice. Further, 5 QTLs for mesocotyl length were detectedusing an RIL population from the cross between 2 japonica cultivars by using the filter paper method (Huang et al. 2010). Cao et al. (2002) detected 8 QTLs on chromosomes 1, 3, 6, 7, 8, and 12 under moderate and low temperate conditions in a doubled haploid population derived from a cross between a japonica cultivar and an indica cultivar. In a previous study, we detected 5 QTLs for mesocotyl length on chromosomes 1, 3, 7, 9, and 12 by using agar media and the same backcross inbred line (BIL) populations used in this study (Lee et al. 2012).
QTLs for improving seedling vigor or seedling establishment, including traits for seedling emergence, seed germination, or shoot length, and coleoptile length have been detected (Redoña and Mackill 1996; Zhou et al. 2007; Xie et al. 2014). Redoña and Mackill (1996) detected 2 QTLs for coleoptile length and 5 QTLs for mesocotyl length by using an F2:F3 population derived from a cross between japonica cultivar Labelle and indica cultivar Black Gora. Further, 5 QTLs for seed vigor traits such as coleoptile length and radicle length and 3 QTLs for seed germination under low and normal temperature conditions were identified using populations derived from a cross between two indica cultivars (Xie et al. 2014). In addition, 9 QTLs for seedling vigor traits, including coleoptile emergence, were detected under two field conditions—drained and flooded soil—and 2 QTLs for coleoptile emergence were identified on chromosomes 1 and 3 under flooded soil condition (Zhou et al. 2007).
In this study we aimed to show that mesocotyl elongation is essential to seedling emergence under deep-seeding using chromosome segment substitution lines (CSSLs). Although several studies identified QTLs for mesocotyl or coleoptile elongation, the locations and effects of the detected QTLs varied according to the test methods (Redoña and Mackill 1996; Zhou et al. 2007; Huang et al. 2010; Xie et al. 2014). Furthermore, no study tried to characterize and detect QTLs for mesocotyl under deep-seeding condition in rice. Also, study to analyze the association between mesocotyl elongation and seedling emergence under various soil depth conditions is rare in crop including rice (Chung 2010; Alibu et al. 2011; Lu et al. 2016).
This study attempted to (1) identify QTLs associated with mesocotyl and coleoptile elongation under the deep-seeding soil condition, and (2) to evaluate the effect of mesocotyl elongation on seedling emergence using deep-seeded CSSLs.
Phenotypic variation of mesocotyl and coleoptile elongation
QTL analysis for deep-seeding tolerance-related traits
Characteristics of QTLs for mesocotyl and coleoptile length in 98 BILs
LOD score c
R2 (%) d
Positive allele e
Effect of mesocotyl elongation on seedling emergence
A positive correlation was found between mesocotyl length and seedling emergence at 6 days after sowing (r = 0.8, P < 0.0001), whereas no significant correlation was noted between coleoptile length and seedling emergence (P > 0.5). This result suggests that seedling emergence is dependent on mesocotyl elongation.
All lines except 5 lines were not significantly different from Nipponbare in mesocotyl and coleoptile lengths (Fig. 4). Four lines, CSSL-5, CSSL-15, CSSL33 and CSSL-54 showed significantly longer mesocotyl length than Nipponbare and the other CSSLs (Fig. 4b). CSSL-5 showed the highest emergence percentage (70%) among CSSLs followed by CSSL-33 (17%) and CSSL-15 (12.5%). (Fig. 4a). CSSL-5 possessed Kasalath segments across qMel-1 and qMel-3 and across only qMel-3 in CSSL-15. On the other hand, CSSL-33 possessed the Kasalath segments across qMel-3 and qMel-7. The qMel-7 QTL was not detected in this study whereas it was observed in the agar condition (Lee et al. 2012). Coleoptile length of Kasalath and CSSL-53 was significantly shorter than Nipponbare and the other CSSLs, but the emergence percentage of Kasalath (87.5%) was the highest (Fig. 4a and C). This result suggests that seedling emergence is dependent on mesocotyl elongation.
Additionally, these results also show that the 2 QTLs, qMel-1 and qMel-3 act additively in distinct or complementary pathways in controlling mesocotyl elongation under this study condition.
In rice, seeding depth for direct seeding is an important factor for ensuring seedling establishment. Lee et al. (2002) recommended a 2–3-cm sowing depth for japonica rice. When the seeding depth is >3 cm, seedling emergence was markedly delayed (Kawatei et al. 1963; Murai et al. 1995; Luo et al. 2007; Chung 2010; Alibu et al. 2012). Moreover, when seeds were sown deeper than 5 cm, the first leaf developed under the soil surface, and seedling establishment decreased remarkably (Chung 2010). Similar result was obtained in this study: seedling emergence speed and percentage at 7 cm and 10 cm soil depths were lower than those at the seeding depth of 3 cm in all the genotypes (Fig. 5). Although 3-cm seeding depth might be optimal for direct seeding based on these results, many studies have used seeding depths of less than 3 cm, which caused seed desiccation and floating and seedling lodging (Lee et al. 2002, Kumar and Ladha 2011). Genotype differences for seedling emergence and coleoptile length were more pronounced at 5 cm than at 3 cm seeding depth (Figs. 1 and 5). The two parents and the 3 CSSLs showed >90% seedling emergence within 7 days when the seeding depth was 3 cm, whereas Kasalath and CSSL-5 showed >90% emergence and the other three lines reached a plateau at 80% emergence at 5 cm seeding depth (Fig. 5). These results indicate that soil depth of 3–5 cm is appropriate for direct seeding of cultivars that show good mesocotyl elongation without affecting seedling establishment. Based on these findings, we selected 5 cm soil depth for screening seedling emergence traits under the deep-seeding condition.
Mesocotyl and coleoptile lengths are directly related with seedling emergence in deep seeding and enhanced mesocotyl or coleoptile elongation is associated with better seedling emergence and establishment (Turner et al. 1982; Murai et al. 1995; Luo et al. 2007; Chung 2010; Alibu et al. 2012). Murai et al. (1995) reported that a relationship exists between seedling emergence ability and lengths of leaf, mesocotyl, coleoptile, and leaf internode using dwarf lines under 7 cm soil depth. Chung (2010) reported positive correlations between the rate of seedling emergence and mesocotyl or coleoptile length at 5 cm seeding depth by using 116 Korean weedy rice accessions. Alibu et al. (2012) suggested that upland rice accessions with long mesocotyl showed better seedling emergence than those with long coleoptile under the dry direct-seeding condition. Lu et al. (2016) showed that long mesocotyl rice accessions had higher emergence rate than that of the short one at 2 and 5-cm sowing depth. In addition to the seeding depth, in dry seeding, the lengths of mesocotyl and coleoptile can be affected by moisture content. Under submergence, coleoptile growth was stimulated, although no increase in mesocotyl length was observed (Takahashi 1978; Alibu et al. 2011). This suggests that mesocotyl and coleoptile elongation is important for seedling emergence depending on the environment. In our study, a positive correlation between mesocotyl length and seedling emergence was observed (Figs. 4 and 5). Seedlings of Kasalath and CSSL-5 that have long mesocotyls emerged considerably faster than those of the other genotypes that have short mesocotyl at the 7 and 10 cm seeding depths (Figs. 4 and 5). At 7 cm seeding depth, seedlings of Kasalath and CSSL-5 emerged over 70% in 7-8 days after sowing, whereas those of the other genotypes and Nipponbare showed 70% emergence at 10–11 days after sowing. This delayed seedling emergence of up to 3–4 days might be unfavorable for seedling establishment and competition with weeds.
Evaluation of RDRS showed genotypic variation of mesocotyl and coleoptile length (Fig. 2). Japonica accessions tend to have shorter mesocotyls than indica, whereas japonica showed the longest coleoptiles among four groups. In this study, indica-I accessions had the longest mesocotyls among the four groups, followed by the tropical japonica group (Fig. 2b). These results are consistent with the previous findings (Suge 1972; Takahashi 1978, Sato 1987).
QTL analysis was conducted for mesocotyl and coleoptile length of seedlings sown at 5 cm soil depth. Measuring mesocotyl and coleoptile traits in the soil is difficult mainly due to the environmental influence and experimental errors. Several studies attempted to map QTLs for mesocotyl and coleoptile length by using the glass tube method (Katsuta-Seki et al. 1996), slant-board test (Redoña and Mackill 1996), filter paper with distilled water (Huang et al. 2010), agar medium (Lee et al. 2012), and filter paper on agar medium (Xie et al. 2014). However, QTL analysis for mesocotyl or coleoptile elongation at the 5 cm seeding depth by using CSSLs in rice has not yet been performed. We detected 5 QTLs under field condition in two replicates. Interestingly, QTLs for mesocotyl elongation were commonly mapped to chromosomes 1 and 3 for different mapping populations and under different experimental conditions. The QTL qMel-3 was located on the long arm of chromosome 3, and this QTL interval overlapped with regions of QTLs reported in previous studies (Katsuta-Seki et al. 1996; Redoña and Mackill 1996; Cai and Morishima 2002; Cao et al. 2002; Huang et al. 2010). Using genome-wide association study (GWAS) association mapping, Wu et al. (2015) and Lu et al. (2016) identified some candidate genes controlling mesocotyl elongation on chromosome 1 and 3. Coleoptile QTLs were detected on chromosomes 3 and 6 by Redoña and Mackill (1996) and on chromosomes 5, 8, and 11 by Xie et al. (2014). On chromosomes 1 and 3, two QTLs for coleoptile length were identified under flooded soil condition (Zhou et al. 2007). Interestingly, qCol-3 and qCol-5 that were detected in the present study did not overlap with the coleoptile length QTLs that was reported previously; this could be attributed to the different genetic materials and test methods used.
Among 54 CSSLs, two lines, CSSL-5 and CSSL-33 showed significantly longer mesocotyl than Nipponbare. CSSL-5 showed the highest emergence ratio (70%) followed by CSSL-33 (17%) (Fig. 4a and b). CSSL-5 and CSSL-33 shared qMel-3 suggesting that qMel-3 is a major QTL. In contrast, qMel-7 is minor and affected by testing methods based on the finding that the qMel-7 QTL was detected in the agar condition (Lee et al. 2012) but not in the soil condition in this study. However, CSSL-5 seedlings were shorter in mesocotyl length and lower in emergence rate than Kasalath. The results from this study indicate that the qMel-3 QTL with a high additive effect has the potential to enhance early seedling emergence in combination with the qMel-1, qMel-7 and other QTLs not detected. Also, information on the interaction among these QTLs will be necessary to understand the genetic control of mesocotyl length. These findings will contribute to our understanding of the genetic control of mesocotyl and coleoptile elongation, and seedling emergence and provides information on QTL regions associated with early seedling emergence for rice breeding programs.
We showed that the variation in mesocotyl elongation is associated with seedling emergence under the deep-seeding condition. Genetic analysis using BIL and CSSL plants confirmed that two major QTLs, qMel-1 and qMel-3 for mesocotyl elongation have the potential to enhance early seedling emergence at 5 cm seeding depth. This is the first report to show the effect of two QTLs for mesocotyl elongation detected both in agar media and deep-seeding soil on seedling emergence using CSSLs using deep-seeding soil condition. (Lee et al. 2012). High-resolution mapping and cloning of these QTLs should be performed to understand the genetic control of mesocotyl elongation to ensure higher seedling emergence and to develop deep-seeding-tolerant rice cultivars for direct seeding by using marker-assisted selection.
Fifty-seven rice accessions selected from the RDRS of germplasm collection were used to detect variation in mesocotyl and coleoptile elongation (Oryza sativa L.) (Kojima et al. 2005; Lee et al. 2012; Additional file 2: Table S1). QTLs for mesocotyl and coleoptile elongation were detected and the effect of mesocotyl elongation on seedling emergence was determined by using 98 BILs and 54 CSSLs developed from the backcross of the Nipponbare/Kasalath (Lin et al. 1998). Seeds of BILs and CSSLs were provided by Rice Genome Resource Center (RGRC), Japan (http://www.rgrc.dna.affrc.go.jp/stock.html).
The effect of QTLs for mesocotyl elongation qMel-1 and qMel-3 on seedling emergence was determined by selecting 3 CSSLs—CSSL-6, CSSL-15, and CSSL-5 (Lee et al. 2012). The genotype data of the CSSLs were referred from a database of RGRC (http://www.rgrc.dna.affrc.go.jp/ineNKCSSL54.html). CSSL-6 and CSSL-15 carried the QTLs qMel-1 and qMel-3, respectively. CSSL-6 contained a 34-cM Kasalath segment flanked by restriction fragment length polymorphism (RFLP) markers C86-C112 carrying qMel-1 on chromosome 1. CSSL-15 contained a 61-cM Kasalath segment flanked by RFLP markers R19–R1925 carrying qMel-3 on chromosome 3 in the Nipponbare background. CSSL-5 carried both QTLs qMel-1 and qMel-3 in the Nipponbare background. In CSSL-5, 2 Kasalath segments on qMel-1 and qMel-3 were introgressed, a 34-cM Kasalath segment near the RFLP makers C1370–2018, including qMel-1, 43-cM Kasalath segment near RFLP markers C136–R1925, in the Nipponbare background.
RDRS, BIL, and CSSL plants were grown in the experimental lowland field of the Graduate School of Life Sciences, Tohoku University, Kashimadai, Osaki, Miyagi Prefecture, Japan. The panicles of these plants were harvested at 40–50 days after heading and then dried in a well-ventilated space for 3 months. Dried seeds were collected from the panicle and placed in paper envelopes. All of the seeds were completely germinated in 30 °C imbibitions for 4 days. For maintaining the germinability, the seed envelopes were packed in plastic bags sealed with silica gel and stored at 4 °C in a refrigerator until use.
Measurement of mesocotyl and coleoptile elongation
Mesocotyl and coleoptile elongation was measured and seedling emergence under the soil condition was evaluated by sowing 12 good-quality seeds from each RDRS accession, BILs, and CSSLs at various soil depths. Seeds of Nipponbare, Kasalath, and 3 CSSLs were buried at a depth of 3, 5, 7, and 10 cm in plastic pots (diameter, 11 cm; height, 15 cm) in soil containing chemical fertilizer (Nursery culture soil No. 3; Mitsui-Toatsu, Japan). For determining phenotype variation in mesocotyl and coleoptile length, seeds of RDRS, 98 BILs and 54 CSSLs were buried in 5 cm soil depth. The plastic pots were kept in plant growth cabinets at alternate temperatures of 30 °C and 26 °C (14 h/10 h). The water level of the soil was maintained 2 cm from the bottom of the pots to maintain adequate soil moisture for germination and seedling growth. At 14 days after sowing, the seedlings were carefully excavated and washed, and their lengths were measured. Seedlings insufficiently grown were excluded from measurement. In this study, measurement was made on seedlings showing 3rd-4th leaves expansions at 14 days after sowing. Mesocotyl (the distance from the basal part of the seminal root to the coleoptilar node) and coleoptile (the distance from the coleoptilar node to the tip of the coleoptile) lengths of each seedling were measured using a ruler. Seedlings that germinated but grew insufficiently were excluded from the length measurements. The numbers of emerged seedlings from the soil surface were counted daily up to 14 days when seedling emergence was thought to be complete. The experiment was performed in a completely randomized design with two or three replicates of each line.
Genotype data of BILs and QTL analysis
The QTLs controlling mesocotyl and coleoptile elongation in BILs were mapped by using genotype data generated using 245 RFLP markers (http://www.rgrc.dna.affrc.go.jp/ineNKBIL98.html). Linkage analysis of the genotypic data was performed using the Kosambi function of Mapmaker/EXP 3.0 software (Lander et al. 1987). QTL analysis was performed using composite interval mapping (CIM) by using the QTL Cartographer version 2.5 software (Wang et al. 2012). CIM analysis was performed using forward-backward stepwise regression model 6 with a 10-cM window size. The LOD threshold significance level (P < 0.05) was determined by computing 1000 permutations. The QTL positions were assigned to the points of maximum LOD score in the target regions. The percentage of total phenotypic variance noted for each QTL was estimated on the basis of the R 2 value.
This work was carried out with the support of and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1A6A3A01060388) and "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ 011048)", Rural Development Administration, Republic of Korea.
HL conceived of the study and carried out molecular genetic analysis and statistical analysis, and wrote manuscript. KS helped to design research and performed statistical analysis. JK and SW advised to draft the manuscript. TS participated in the design of the study. SA designed research and helped to draft the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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