Fig. 6

Pollen viability and setting rate of rice cv. Kitaake and EBE-edited lines. A Growth and development of Kitaake and homozygous, EBE-edited rice lines in the field. B Representative images of pollen viability tests from Kitaake and edited lines. Viable and sterile pollen grains stained dark and light yellow. C, D Statistical analysis of pollen viability (n = 10) and setting rates (n = 10) of Kitaake and EBE-edited lines. The percentage of pollen viability was calculated relative to total pollen counts obtained from eight microscopic images. The seed setting rate was calcuated as the percentage of solid grains relative to the total number of grains