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Rice
Fig. 5 | Rice

Fig. 5

From: The Mediator Subunit OsMED16 Interacts with the WRKY Transcription Factor OsWRKY45 to Enhance Rice Resistance Against Magnaporthe oryzae

Fig. 5

OsWRKY62 interacts with OsWRKY45, but not with OsMED16. A Y2H assays showed that OsWRKY62-1 and OsWRKY62-2 interacted with OsWRKY45, OsWRKY62-1 and OsWRKY62-2 did not interact with OsMED16. Positive interaction between colonies of transformed yeast cells grown on synthetic glucose (SD) medium, grown on SD-Trp-Leu-His and SD-Trp-Leu-His-Ade. 10–2, diluted 100 × 10–3, diluted 1000 times. p53-BD/SV40-T antigen-AD was used as positive control, Lam-BD/SV40-T antigen-AD was used as negative control. B BIFC analysis showing that the interaction between OsWRKY62-1-cYFP,OsWRKY62-2 and OsWRKY45-nYFP forms a functional YFP in the nucleus. RFP: nuclear localization Marker RFP fluorescent signal; Merge: fused YFP with RFP fluorescent signal. All assays have been repeated at least three times with similar results. bar = 20 μm

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