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Fig. 2 | Rice

Fig. 2

From: The Mediator Subunit OsMED16 Interacts with the WRKY Transcription Factor OsWRKY45 to Enhance Rice Resistance Against Magnaporthe oryzae

Fig. 2

OsMED16 interacts with OsWRKY45 in the nucleus. A Y2H assays showed that OsMED16 interacted with OsWRKY45. PGADT7 did not interact with OsWRKY45-BD indicating that OsWRKY45-BD cannot auto-activate the reporter in yeast (Saccharomyces cerevisiae). Transformed yeast cells were grown on synthetic glucose (SD) medium, and growing colonies on SD-Trp-Leu-His and SD-Trp-Leu-His-Ade indicate positive interactions 10–2, diluted 100 times. 10–3, diluted 1000 times. p53-BD/SV40-T antigen-AD was used as positive control, Lam-BD/SV40-T antigen-AD was used as negative control. B BIFC analysis showing that the interaction between OsMED16-cYFP and OsWRKY45-nYFP forms a functional YFP in the nucleus. RFP: nuclear localization Marker RFP fluorescent signal; Merge: fused YFP with RFP fluorescent signal. All assays have been repeated at least three times with similar results. bar = 20 μm

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