Fig. 1
OsMED16 positively regulates rice resistance against M. oryzae. A Expression of OsMED16 in WT, OE-12,OE-16 and med16-11, med16-26 plants was analyzed by RT-qPCR. Values were taken as mean ± SD, n = 3, and OsActin was used as a reference gene for normalization. B The results of Sanger sequencing indicated that the single strand of the sgRNA1 region in med16-11 and med16-26 genomic DNA contained deletions of 4 nt and insertions of 1 nt, respectively, compared to the wild type. C OsMED16 overexpression (OE-12, OE-16) enhanced resistance to M. oryzae, and CRISPR-Cas9 plants (med16-11, med16-26) reduced resistance to M. oryzae. WT, OE-12, OE-16 and med16-11, med16-26 plants were inoculated with M. oryzae Guy11, and images were taken at 5 dpi. bar = 1 cm. D Lesion area of leaves in WT, OE-12, OE-16, and med16-11, med16-26 after inoculation. The values are mean ± SD (n = 15); **p value < 0.01, Student's t-test. E Quantitative detection of rice blast fungus growth in WT,OsMED16 overexpression and knockout plants 7 days after infection with strain Guy11. The values are mean ± SD (n = 15); **p value < 0.01, Student's t-test