Fig. 7

Sequence variations of LOC_Os10g22484 and LOC_Os10g22520 in high LTG and low LTG rice accessions. a Three predicted transcripts of LOC_Os10g22484 from RGAP. Genomic analysis indicated that the sequences after 3969 bp were variated in 517 and S18, compared to 86 and HJX74. A pair of primers stretching across the variation joint were designed as D3F/D2R. b The amplified products of DNA from the selected 20 rice accessions using primer D3F/D2R revealed by agrose gel electrophoresis. c Gene structure of LOC_Os10g22520. + 1 indicates the site of initiation codon. Black rectangles, white rectangles and gray arrow indicate the exons, 3′ and 5′ UTR, and insertion sequence, respectively. A pair of primer was designed to amplify the sequence between + 351 to − 443 (designated as 10gT1). d Sequence alignment of 10gT1 in LOC_Os10g22520 between high and low LTG rice accessions. The sequence of 10gT1 in Nipponbare (NPB) was taken as reference. The sequences from + 277 to + 268 were deleted in low LTG lines. A fragment of 239 or 256 bp was inserted after − 179 bp upstream of initiation codon in high LTG lines