Fig. 3

OsProDH negatively regulates thermotolerance in rice a Transcript levels of OsProDH detected by qPCR in KY131 and transgenic two overexpression (OE) lines in shoots at the two-leaf stage rice seedlings. The data shown are the mean values of three technical repeats with the SD. b Gene structure of OsProDH and sequencing results at target sites in T1 plants produced by CRISPR/Cas9. c Proline contents of KY131, OE and CRI lines in shoots at the two-leaf stage rice seedlings under normal conditions. Values are the means ± SE, n = 3. Differences between the KY131 and transgenic lines were analyzed with Student’s t-test. (*P < 0.05). d Phenotypes of KY131, OE and CRI lines under 45 °C treatment. The two-leaf stage seedlings were subjected to high temperature treatment for 48 h and then recovered at normal conditions. e Survival rates of KY131, OE and CRI lines after recovering 15 days at normal conditions. (n = 3 × 20). f Proline contents of KY131, OE and CRI lines in shoots at the two-leaf stage rice seedlings after 45 °C treatment for 48 h. Values are the means± SE, n = 3. Differences between the KY131 and transgenic lines were analyzed with Student’s t-test. (*P < 0.05; **P < 0.01). g DAB staining of KY131, OE and CRI lines leaves from plants under normal (left) and stressed (right, 45 °C) conditions, respectively