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Correction to: A Versatile Vector Toolkit for Functional Analysis of Rice Genes

The Original Article was published on 20 April 2018


The caption of Fig. 5 contained an error. The updated caption along with the original figure is published in this correction article.

Fig. 5
figure 1

Diagram of how CRISPR/Cas9 vectors were constructed and used to edit the rice IPA1 gene. a Western blot analysis of the Cas9-intron expression in rice protoplasts. HSP indicates the loading amount of each sample. b Target site selection for candidate genes in the rice genome. A 20-bp specific sequence followed by the PAM “NGG” structure is required. c and d Target cloning to the entry vectors. Synthesis of the primer pairs of the 20-bp specific target with the 4-bp adapters, and ligation with the BsaI linearized pEntry A or pEntry B vector. e One-step ligation and f step-by-step ligation of multiple targets to pRHCas9/pRGCas9. Four pairs of isocaudamers, PstI(E1)-NsiI(E1'), XbaI(E2)-SpeI(E2'), BamHI(E3)-BglII(E3'), and SalI(E4)-XhoI(E4') are marked. The sgRNA cassettes with U6P1 and U6P2 in pEntry A and B should be used in turn. g Representative sequencing chromatogram of the CRISPR-IPA1 transgenic lines. Line #7, wild-type genotype; line #5, mutant genotype. h Representative gene editing results in the CRISPR-IPA1 transgenic lines

Table S1 in the Additional file of this original publication contained some errors. The updated Table S1 is published in this correction article.


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Correspondence to Yuese Ning or Guo-Liang Wang.

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The original article can be found online at

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Updated Table S1, the full supplementary materials can be downloaded from the original publication. (XLSX 10 kb)

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He, F., Zhang, F., Sun, W. et al. Correction to: A Versatile Vector Toolkit for Functional Analysis of Rice Genes. Rice 11, 49 (2018).

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