Fig. 4

The 08sg2 mutant is insensitive to 24-epiBL. a Lamina joint of R498 and 08sg2 in response to 24-epiBL treatment. b Comparisons of the mesocotyls elongation between R498 and 08sg2 when plants were grown in complete darkness, arrows indicate the nodes. c Roots of R498 and 08sg2 seedlings grown on ½MS medium without (left) or with (right) 1 μM 24-epiBL treatment. d The coleoptile elongation of R498 and 08sg2 without (left) or with (right) 1 μM 24-epiBL treatment. e Measurements of the lamina joints angles of the wild type and 08sg2 after treatment with various concentrations of 24-epiBL. f The mesocotyls length of R498 and 08sg2 when plants were grown in complete darkness. g Roots length of R498 and 08sg2 seedlings grown on ½MS medium with 1 μM 24-epiBL treatment. h Coleoptile length of R498 and 08sg2 seedlings grown on ½MS medium with 1 μM 24-epiBL treatment. Bars = 10 mm. i The analysis of relative expression level was determined by qRT-PCR using young panicles (5-7 cm) during the inflorescence development. OsActin was used as the control and the values of expression levels in R498 were set to one (n = 3). Data are given as means ± SD. Student’s t-test was used to generate the P values; *, ** and NS indicate P < 0.05, P < 0.01 and no significant differences, respectively