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Rice
Fig. 7 | Rice

Fig. 7

From: Development and validation of allele-specific SNP/indel markers for eight yield-enhancing genes using whole-genome sequencing strategy to increase yield potential of rice, Oryza sativa L.

Fig. 7

GS5 markers. a The selected DNA polymorphisms in the GS5 promoter region for marker development. The location of the 4-bp indel is 320-bp distance from the translation initiation codon. The C/T SNP was used for designing GS5-03SNP (PCR-gel-based marker) and GS5-03SNP-FD (Fluidigm genotyping marker) markers. b Capillary electrophoresis image from the parental lines analyzed by the GS5-indel1 marker. Aikawa1 and ST6 were used as donors of GS5-WG allele. The WG, MG, and NG alleles of the GS5 gene were designated as W, M, and N, respectively. c Agarose gel image analyzed by the GS5-03SNP marker from the parental lines. d Application of the GS5-03SNP marker in intermediate breeding line. Fourteen BC2F2 plants derived from PR37951 x ST6 cross were tested

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