Fig. 4

Enzymatic activity of OsAAH in vitro and metabolite levels of ureide catabolism in developing seeds. A The pathway of ureide catabolism. B AAH-GFP and GFP purified from 35 S: OsAAH-GFP and 35 S: GFP transgenic plants, respectively, were analyzed by western blot with GFP antibody. C Enzyme activity of OsAAH in vitro. GFP was used as a negative control. D Enzyme activity was identified by measuring absorbance at 520 nm. Data are means ± SD (n = 3). Significance analysis was conducted with Student’s t-test (**P < 0.01). E, F Allantoin (E) and allantoate (F) contents in developing seeds harvested at 20 and 25 DAP from WT, phs39, Osaah mutants and the complementation lines. Data are means ± SD (n = 3). Different letters indicate significant differences at P < 0.05 determined by one-way ANOVA