A Case Study from the Overexpression of OsTZF5, Encoding a CCCH Tandem Zinc Finger Protein, in Rice Plants Across Nineteen Yield Trials

Grondin, Alexandre; Natividad, Mignon A.; Ogata, Takuya; Jan, Asad; Gaudin, Amélie C. M.; Trijatmiko, Kurniawan R.; Liwanag, Evelyn; Maruyama, Kyonoshin; Fujita, Yasunari; Yamaguchi-Shinozaki, Kazuko; Nakashima, Kazuo; Slamet-Loedin, Inez H.; Henry, Amelia

doi:10.1186/s12284-024-00705-z

Rice

Table 1 Description of trials

From: A Case Study from the Overexpression of OsTZF5, Encoding a CCCH Tandem Zinc Finger Protein, in Rice Plants Across Nineteen Yield Trials

Environment season	Lines included	Generation	Trial	Treatment	Draining date (das)	Temp (ave.°C)
Transgenic trials
Screenhouse DS2011	IR64, 1-TZF5-13, 1-TZF5-24, 1-TZF5-72	T₁	SH1-W	Well-watered	–	28
	IR64, 1-TZF5-13, 1-TZF5-24, 1-TZF5-72		SH1-VRS	Veg + Repro Stress	44	29
Field DS2012	IR64, 0-TZF5-24, 1-TZF5-13, 1-TZF5-24, 1-TZF5-72, 14-1-2-10 BIL	T₂	F-W	Well-watered	–	28
			F-VRS	Veg + Repro Stress	45	28
			F-RS	Repro Stress	60	28
Screenhouse WS2012	IR64, 1-TZF5-72, 14-1-2-10 BIL	T₃	SH2-W	Well-watered	–	27
	IR64, 1-TZF5-72, 14-1-2-10 BIL		SH2-VRS	Veg + Repro Stress	36	28
Screenhouse WS2013	IR64, 0-TZF5-13, 0-TZF5-24, 0-TZF5-72, 1-TZF5-13, 1-TZF5-24, 1-TZF5-72, 14-1-2-10 BIL	T₂–T₅	SH3-W	Well-watered	–
			SH3-VRS	Veg + Repro Stress	53
Screenhouse DS2015	IR64, 0-TZF5-13, 0-TZF5-24, 0-TZF5-72, 1-TZF5-13, 1-TZF5-24, 1-TZF5-72, 14-1-2-10 BIL	T₃–T₅	SH4-W	Well-watered	–	27
			SH4-VRS	Veg + Repro Stress	49	28
Screenhouse DS2014	IR64, 1-TZF5-13, 1-TZF5-24, 1-TZF5-72, 14-1-2-10 BIL	T₄–T₆	SH5-W	Dry direct seeded, Well-watered	–
	IR64, 1-TZF5-13, 1-TZF5-24, 1-TZF5-72, 14-1-2-10 BIL		SH5-SS	Dry direct seeded, Seedling Stress	10
Cylinder WS2013	IR64,0-TZF5-13, 1-TZF5-13	T₃–T₆	C1-W	Well-watered	–	29
	IR64,0-TZF5-13, 1-TZF5-13		C1-VS	Veg Stress	22
Cylinder DS2014	IR64, 0-TZF5-24,0-TZF5-72, 1-TZF5-24,1-TZF5-72	T₃–T₅	C2-W	Well-watered	–	31
	IR64, 0-TZF5-24,0-TZF5-72, 1-TZF5-24,1-TZF5-72		C2-VS	Veg Stress	22
Background-cleaned trials
Cylinder WS2016	IR64, 0-TZF5-13, 0-TZF5-24,0-TZF5-72, 1-TZF5-13, 1-TZF5-24,1-TZF5-72, 3 BC₃F₁ lines (+), 3 BC₃F₁ lines (−),14-1-2-10 BIL	BC₃F₁	C3-W	Well-watered	–	28
			C3-VS	Veg Stress	26	28
Screenhouse WS2017	IR64, 0-TZF5-13, 0-TZF5-24,0-TZF5-72, 1-TZF5-13, 1-TZF5-24,1-TZF5-72, 3 BC₃F₂ lines (+), 3 BC₃F₂ lines (−),14-1-2-10 BIL	BC₃F₂	SH6-W	Well-watered	–
			SH6-RS	Repro Stress	42
Pyramiding trials
Screenhouse WS2016	IR64, 1-TZF5-24,1-TZF5-72, 36 BC2F3 lines (+), 10 BC₂F₃ lines (−), IR87707-445-B-B-B	BC₂F₃	SH7-W	Well-watered	–	27
			SH7-RS	Repro Stress	48	28
Screenhouse DS2017	IR64, 1-TZF5-72, 9 BC₂F₄ lines (+), 1 BC₂F₄ lines (−), IR87707-445-B-B-B	BC₂F₄	SH8-W	Well-watered	–	31
			SH8-RS	Repro Stress	41	31
Screenhouse DS2018	IR64, 1-TZF5-72, 9 BC₂F₅ lines (+), 1 BC₂F₅ lines (−), IR87707-445-B-B-B	BC₂F₅	SH9-W	Well-watered	–	28
			SH9-RS	Repro Stress	56	28

SH Screenhouse, F Field, C Cylinder, DS dry season, WS wet season
IR64: drought susceptible wild-type, 14-1-2-10 BIL and IR87707-445-B-B-B: drought tolerant QTL lines in the background of IR64, 1-TZF5-13, 1-TZF5-24, 1-T-TZF5-72: transgenic lines, 0-TZF5-13, 0-TZF5-24, 0-T-TZF5-72: azygous null lines, W: well-watered, RS: reproductive stage drought stress, VRS: vegetative and reproductive stage drought stress, VS: vegetative stage drought stress, SS: seedling stage drought stress, das: days after sowing

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