Fig. 3
OsTZF5 expression in the drought susceptible wild type IR64 and 1-TZF5 transgenic lines at the T3 and T5 generations and in pyramided lines at the BC2F5 generation. Transgenic lines from cylinder trial C2: A OsTZF5 endogenous and transgene expression in the well-watered and drought stress treatments at 16 days after draining (38 days after sowing) using the OsActin1 gene as reference (*p < 0.05 from well-watered conditions). Transgenic lines analyzed at JIRCAS: B Northern blot of OsTZF5 expression, and real-time quantitative PCR using primers detecting C OsTZF5 endogenous gene and transgene expression and D) transgene specific expression with 18S rRNA gene as a reference in both cases (**p < 0.01 and ns means no significance from Day 0). Pyramiding lines: real-time quantitative PCR using primers detecting E OsTZF5 endogenous gene and transgene expression and F transgene specific expression with OsUbi1 gene as a reference in both cases (ns means no significance from IR64). Primers are described in Tables S6 and S7. Bars represent mean values ± se of three biological replicates