Fig. 7
From: The RING Domain of Rice HEI10 is Essential for Male, But Not Female Fertility
The RING domain of HEI10 is not required for nuclear localisation and interaction with other meiotic proteins. A Yeast two-hybrid assay of interaction between rice HEIP1, PTD, ZIP4, MSH5 and full-length HEI10 or short HEI10 (sHEI10). AD, activating domain; BD, DNA-binding domain; -2 SD, synthetic defined medium without leu and trp showing co-transformation of constructs; -4 SD, synthetic defined medium without leu, trp, ade, and his showing interaction of expressed proteins. B Subcellular localisation of short HEI10 tagged with GFP in tobacco leaf epidermal cells. “Merge” combines fluorescence and bright field images. Scale bars = 25 μm