Fig. 6
From: The RING Domain of Rice HEI10 is Essential for Male, But Not Female Fertility
Recombinant short HEI10 partially restores female fertility in sh1. A Top: Schematic representation of the SH1 locus containing the coding region of the presumed short HEI10 and its 3 kb upstream sequence. Yellow boxes, HEI10 exons 3–8. Bottom: Schematic arrangement of the maize ubiquitin promoter (Ubi) driving expression of the short HEI10 coding sequence (CDS), terminated by a NOS terminator. The second start codon and stop codon of the HEI10 CDS are indicated. B Seed setting rate of SH1-gDNA;hei10 and Ubi:SH1cds;hei10 transgenic panicles pollinated by wild-type pollen. For each line, 2 panicles in each of 5 independent plants were pollinated. C Mature embryo sacs from SH1-gDNA;hei10 and Ubi:SH1cds;hei10 transgenic plants. Scale bars = 50 μm. D Embryo sac viability in SH1-gDNA;hei10 and Ubi:SH1cds;hei10 transgenic lines. 50 embryo sacs from 5 independent plants were examined for each line. Data show mean ± SD