Fig. 2

Map-based cloning and analysis of sh1 locus. A Fine mapping of the sh1 locus on chromosome 2 (top), and schematic representation (bottom) of the exon and intron organisation of OsFYVE4 (LOC_02g13890) and HEI10 (LOC_02g13810). Names and positions of the molecular markers are shown. Black boxes indicate exons; intervening lines indicate introns; white squares indicate insertions with relevant genes named. ATG, translational start site. B Schematic representation of the 76 kb inversion fragment in sh1. Light green and dark green arrows indicate the DNA fragments of OsFYVE4 outside and inside the inversion, respectively; dark yellow and light yellow arrows indicate the DNA fragments of HEI10 the DNA fragments inside and outside the inversion, respectively. The primers used for mutant genotyping were indicated by black arrows. C Schematic representation of the exon and intron organisation of the recombined HEI10 locus. Green boxes indicate OsFYVE4 exons (1, 2, and part of 3); yellow boxes indicate HEI10 exons (3–8)