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Fig. 2 | Rice

Fig. 2

From: Small Papillae Regulated by SPD25 are Critical for Balancing Photosynthetic CO2 Assimilation and Water Loss in Rice

Fig. 2

Map-based cloning of SPD25. (a) Fine mapping of SPD25. (b) Structure of the candidate gene LOC_Os05g38000. The red arrow represents the mutation site of SPD25 in spd25, CRI-1 and CRI-2. (c) Structures of the SPD25 protein in WT and spd25. The SPD25 protein contains a PRONE domain. (d) Phenotype of WT, spd25 and complementation lines at early tillering stage. Com, Complementation. Bars = 5 cm. (e) Phenotype of CRISPR/Cas9 transgenic lines at early tillering stage. CRI, CRISPR/Cas9. Bars = 5 cm. (f) Relative expression level of SPD25. Shown are mean ± SD from three biological replicates each containing ten plants. * and ** indicate P < 0.05 and P < 0.01, Student’s t-test

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