Fig. 6

Loss of WS1 function causes defective callose deposition during microsporogenesis. A–F Aniline blue stained anther sections of WT (A–C) and ws1-1 (D–F) from stage 8a (S8a) to stage 9 (S9). A1–F1 Magnified views of the boxed regions in (A–F) to show details. G–R Aniline blue-stained meiotic microspores in the WT (G–L) and ws1-1 (M–R); staining of WT microspores in dyads (G, H) and tetrads (I–L); staining of ws1-1 microspores of the dyad (M, N) and tetrad (O–R). Bright-field in (H, J, L, N, P, R). Bars = 10 μm