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Rice
Fig. 4 | Rice

Fig. 4

From: Glucan Synthase-like 2 is Required for Seed Initiation and Filling as Well as Pollen Fertility in Rice

Fig. 4

Positional cloning and functional confirmation of WS1. A Result of WS1 mapping by linkage analysis. The number of recombinants at each marker is shown below the horizontal bar. WS1 was mapped to a region between markers InDel6 and InDel8. B Results of WS1 mapping by BSA-seq. The peak tip suggested the most probable position of WS1. C Results of candidate gene identification. An SNP (indicated by arrow) was found in WS1, with transversion of A to T in ws1-1, leading to a change of Lysine codon to a premature stop codon. D, E Functional validation of WS1 by genetic complementation and gene editing. CO, genetic complementation plant; ws1-2, WS1 knockout mutant generated by CRISPR/Cas9 editing. The WT phenotype was recovered by genetic complementation in the CO plant, whereas ws1-2 showed a mutant phenotype similar to ws1-1. F–I GSL5CT-GFP was found to be localized to the plasma membrane. WS1-GFP and PIP2:DsRed (Plasma membrane marker) were cotransformed into rice protoplasts. Scale bars = 5 μm

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