Fig. 7

Phenotypic analysis of osmyb103 osccrl1 double mutant transgenic plants. A, B The relative expression comparison of OsMYB103 and OsCCRL1 between the wild-type and osmyb103 osccrl1 double mutant anthers. Data are means ± SD (n = 3). Asterisks indicate the significance differences determined by Student’s t-test (*, 0.01 ≤ P < 0.05; **, P < 0.01). C Spikelet, anther and pollen stained by I2/KI solution of wild type, osmyb103 mutant, osccrl1 mutant, and osmyb103 osccrl1 double mutant. Scale bars, 2 mm (c-1 to c-4); 500 μm (c-5 to c-8); 200 μm (c-9 to c-12). D The scanning electron microscopy of anther, anther epidermis surface, anther inner surface in wild type, osmyb103 mutant, osccrl1 mutant, and osmyb103 osccrl1 double mutant. Scale bars, 200 μm (d-1 to d-4); 50 μm (d-5 to d-12)