Fig. 6

OsMYB103 regulates the expression of OsCCRL1. A, B The relative expression comparison of OsCCRL1 and LOC_Os09g32020.1 between the wild-type and osmyb103 mutant anthers. Data are means ± SD (n = 3). Asterisks indicate the significance differences determined by Student’s t-test (*, 0.01 ≤ P < 0.05; **, P < 0.01). C Transient expression assay of the LUC reporter gene with the multiple cloning site (MCS) effector plasmid using as a control. Data are means ± SD (n = 3). Asterisks indicate the significance differences determined by Student’s t-test (*, 0.01 ≤ P < 0.05; **, P < 0.01). D Schematic diagrams of the distribution of ‘AACC’ motifs (cis-elements recognized by OsMYB103 transcription factors) in the OsCCRL1 promoter regions. E A ChIP-qPCR analysis of OsMYB103 binding the ‘AACC’ motifs to OsCCRL1 promoter regions in Ubi::OsMYB103-GFP plants with anti-GFP antibody. Data are means ± SD (n = 3). F An EMSA assay of OsMYB103 protein directly binding to the probe ‘P2s’ (93 bp) and ‘P7s’ (40 bp) of OsCCRL1 promoter. ‘Biotin-P2s mutant’ and ‘Biotin-P7s mutant’ mean the mutant probes at ‘AACC’ motifs