Fig. 4

Expression analysis and subcellular localization of OsCCRL1. A, B Abundance of OsCCRL1 transcripts in wild-type different tissues and anthers at different developmental stages. Data are means ± SD (n = 3). C mRNA in situ hybridization of OsCCRL1 transcripts in the wild-type anther. Scale bars, 50 μm. D Subcellular localization of 2 × 35S::GFP and 2 × 35S::OsCCRL1-GFP in rice protoplasts. OsH2B-mCherry was used to indicate the nucleus. Scale bars, 5 μm. E Subcellular localization of 35S::GFP and 35S::OsCCRL1-GFP with DAPI in Nicotiana benthamiana. Scale bar: 5 μm. F Immunoblotting of extracted nuclear proteins from tobacco leaves expressing OsCCRL1-GFP using anti-GFP antibodies and Histone antibodies. Histone antibodies was used as the internal reference antibody. G Immunoblotting of extracted cytoplasm proteins from tobacco leaves expressing OsCCRL1-GFP using anti-GFP antibodies and Tubulin antibodies. Tubulin antibodies was used as the internal reference antibody.