Fig. 3
From: OsCCRL1 is Essential for Phenylpropanoid Metabolism in Rice Anthers
Molecular cloning, complementary validation of OsCCRL1, and CRISPR-Cas9-mediated targeted mutagenesis of OsCCRL1. A Map-based cloning of OsCCRL1 on chromosome 9. The thick black line represents the chromosome, the markers used for gene mapping are indicated above the black line, numbers in brackets below the black line represent recombinants, and the value below the line represents the genetic distance (cM) between two markers. The osccrl1 mutant harbors a nucleotide ‘T’ to ‘A’ transition in exon 4, leading to a Val-to-Glu amino acid conversion at site 231. B Anther morphology and pollen grains stained by I2/KI solution in the wild type, osccrl1 mutant and OsCCRL1-complementary transgenic plant. Scale bars, 2 mm (b-1, b-3 and b-5); 200 μm (b-2, b-4 and b-6). C Reference sequence and sequencing of OsCCRL1cas9 mutant lines. D Spikelet, anther, anther dehiscence and pollen grains stained by I2/KI solution in wild type and OsCCRL1cas9 mutant lines. Scale bars, 2 mm (d-1 to d-6); 500 μm (d-7 to d-12); 400 μm (d-13 to d-18); 200 μm (d-19 to d-24)