Fig. 2

Semi-thin section and TUNEL assay of the wild type and osccrl1 mutant. A − N Semi-thin sections of anthers at stages 8a to 13 in the wild type and osccrl1 mutant. The images show a single locule of an anther. E, epidermis; En, endothecium; M, middle layer; T, tapetum; Dy, dyad cell; Tds, tetrads; Msp, microspore; BP, bicellular pollen; MP, mature pollen; dMP, mature pollen. Scale bars, 25 μm. O − X TUNEL analyses of wild-type and osccrl1 anthers at stages 8a to 11. Propidium iodide staining is indicated by red fluorescence and TUNEL positive staining is indicated by yellow to green fluorescence (arrow indication). Scale bars, 100 μm