Fig. 6

Accumulation of phytoalexins in leaves of rice plants that have been grown under high Fe supply. A Methylerythritol phosphate (MEP) and diterpenoid phytoalexin biosynthesis pathways in rice. Genes whose expression is up-regulated by M. oryzae infection in control and high-Fe plants are indicated in purple color. The full name and details on the expression of these genes can be found in Additional file 6: Table S5. B Heat map showing expression levels (row scaled FPKM, fragments per kilobase per million reads) in leaves of Control and High-Fe plants, mock-inoculated and M. oryzae-inoculated plants (48hpi). Gene expression is represented from pale yellow (less expressed) to brown (more expressed). C Accumulation of phytoalexins, diterpenoid phytoalexins and sakuranetin, in leaves of Control and High-Fe plants at 48 hpi and 72 hpi with M. oryzae spores: momilactones (A and B), phytocassanes (B, C, and E), and the flavonoid phytoalexin sakuranetin (−, mock-inoculated; +, M. oryzae-inoculated). Data are mean ± SEM of three biological replicates each with five plants. Asterisks indicate statistically significant differences calculated by two-way ANOVA (*** P ≤ 0.001). G3P, glyceraldehyde-3-phosphate; DXP, 1-deoxy-D-xylulose 5-phosphate; MEP, 2- C-methyl-D-erythritol 4-phosphate; CDP-ME, 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol; CDP-ME2P, 2-phospho-4-(cytidine 5′- diphospho)-2-C-methyl-D-erythritol; MECDP, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; HMBDP, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate; IPP, isopentenyl diphosphate; DMAPP, dimethylallyl diphosphate; GGDP, geranylgeranyl diphosphate; and CDP, copalyl diphosphate