Fig. 2

Differentially expressed genes in leaves of High-Fe plants relative to Control plants. A Number of genes that are up-regulated and down-regulated genes in leaves of rice plants treated with high Fe for 5 days. B Heatmap showing the expression of genes involved in Fe homeostasis, ROS metabolism and biotic stress identified as differentially expressed by RNA-Seq analysis. The expression level (row scaled FPKM, fragments per kilobase per million reads) is represented from pale yellow (less expressed) to brown (more expressed). Fold change (FC) values are represented on the right, in purple (up-regulated genes, Log₂ FC ≥  + 0.5) and green (down-regulated genes, Log₂ FC ≥  − 0.5). Data represented correspond to the mean of two biological replicates, each biological replicate consisting in a pool of 5 leaves from individual plants. The full gene name and ID of DEGs are indicated in Additional file 2: Table S1. C RT-qPCR analysis of DEGs in High-Fe plants relative to Control plants. Data are mean ± SEM (n = 4 biological replicates, including the two replicates used for RNA-Seq analysis). Asterisks indicate statistically significant differences (t-test, *, P < 0.05; **, P < 0.01). Gene-specific primers are listed in Additional file 9: Table S8