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Rice
Fig. 1 | Rice

Fig. 1

From: Simultaneous Enhancement of iron Deficiency Tolerance and Iron Accumulation in Rice by Combining the Knockdown of OsHRZ Ubiquitin Ligases with the Introduction of Engineered Ferric-chelate Reductase

Fig. 1

The vector structure and metal concentrations of the transgenic lines under Fe sufficiency. A HRZ2i + Refre transformant vector structure. The transformed binary vector contained the depicted HRZ2i and the Refre cassettes, along with hygromycin and kanamycin resistance genes inside the T-DNA region of the pIG121Hm vector backbone (Hiei et al. 1994). OsAct1, rice Actin1 gene; 3’UTR, 3’ untranslated region; NOS ter, Agrobacterium tumefaciens nopaline synthase terminator. The vector is not drawn to scale. B Fe and Zn concentrations in the brown seeds after pot culture in Fe-sufficient soil. The following seeds were analyzed: NT, non-transformants; HRZ2i + Refre, T1 seeds of lines 1, 2, 4, 5, 7, and 9, and T2 seeds of line 2–2; HRZ2i, T2 seeds of line 3 (Kobayashi et al. 2013). Means and standard deviation (SD) are shown (n = 7 for NT, n = 6 for HRZ2i + Refre line 2 and 5; n = 5 for HRZ2i line 3; n = 4 for HRZ2i + Refre line 7 and 9; n = 3 for HRZ2i + Refre line 1, 4, and 2–2). DW: dry weight. Asterisks indicate significant differences compared to the NT (two-sample Student’s t-test; *P < 0.05; **P < 0.01)

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