Fig. 1
From: OsWHY1 Interacts with OsTRX z and is Essential for Early Chloroplast Development in Rice
OsWHY1 interacts with OsTRX z in vitro and in vivo. a Interaction of OsWHY1 and OsTRX z in yeast two-hybrid assays. -LT: selective medium (SD/-Leu/-Trp); -LTHA: selective medium (SD/-Leu/-Trp/-His/-Ade); -LTHA + X-α-gal: selective medium (SD/-Leu/-Trp/-His/-Ade) containing X-α-gal. P53-BD and SV40-AD were used as positive controls. b Pull-down assays for detection of interaction of His-OsWHY1 and GST-OsTRX z in vitro. c Bimolecular fluorescence complementation (BiFC) assays demonstrating that OsWHY1 interacts with OsTRX z in Nicotiana benthamiana leaf cell chloroplasts. Bar = 50 μm