Fig. 4

OsSKL2 promotes tolerance to salt and drought stress by regulating H₂O₂ homeostasis in rice. Leaves of four-leaf-stage wild-type (WT) and OsSKL2 transgenic seedlings were treated with 25% PEG for 5 d or 140 mM NaCl for 7 d then measured for A H₂O₂, B MDA, C SOD, D POD and E CAT enzyme activity. Data represent means ± SD. F H₂O₂ histochemical analyses of 10-day-old seedlings treated with 250 mM mannitol or 140 mM NaCl then stained with 1% 3,3’-diaminobenzidine tetrachloride (DAB) (bar = 1 cm). G Phenotypes of WT and OsSKL2 transgenic plants before (upper) and after treatment with 100 mM H₂O₂ for 10 d (lower) (bar = 5 cm). H Seedling height and I the seminal root number of WT and OsSKL2 transgenic plants under 100 mM H₂O₂ stress. Data represent means ± SD (n = 30). Three independent experiments were carried out with similar results. All data were analyzed using one-way analysis of variance (ANOVA) based on the Student’s t-test. *P < 0.05, **P < 0.01