Fig. 3
From: Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice
Responsibility of OsFPN1 for the phenotype of 1187_n. A Exon intron structure of OsFPN1. Gray boxes, dark boxes, and lines represent the untranslated region, exon, and intron, respectively. OsFPN1 of 1187_n has a nonsynonymous mutation of T to G [leucine (L) is replaced with glutamine (G)]. Osfpn1-2 and osfpn1-3 are CRISPR/Cas 9 mutants with 1 bp and 26 bp deletions at the first exon of OsFPN1, respectively. WT (Nipponbare) is the background of the mutants. B Scatterplot based on the Co and Ni concentrations in the shoots of three-week-old seedlings of Hitomebore, 1187_n, and F2. F2_Wild, F2_Hetero, and F2_Mutant represent the wild-type, heterozygous, and mutant homozygous genotypes of F2, respectively. C Co and Ni concentrations in the shoots of Hitomebore, osfpn1-1, Nipponbare, osfpn1-2, and osfpn1-3. Plants were grown in Kimura B solution supplied with 10 trace elements. Data represent the means ± SD (n = 3–12). Student’s t-test (compared to Hitomebore or Nipponbare). **p < 0.01