Fig. 1
From: The Methylation Inhibitor 5-Aza-2′-Deoxycytidine Induces Genome-Wide Hypomethylation in Rice
Methylation status was significantly reduced after AzaD treatment. a Boxplot of global methylation levels of CG, CHG, and CHH methylation in AzaD treated and osmet1-2 mutant rice. Methylation levels of the three contexts showed significant reductions in both AzaD treated and osmet1-2 rice. b Fold changes of methylation level for CG, CHG, and CHH methylation on chromosome 4. AzaD treatment induced moderate CHH methylation increases in the heterochromatin region. The grey boxes mark the centromere regions. c Metaplot of methylation levels in genes, TE bodies and their flanking regions. d Relative expression levels of selected methyltransferases. Transcript levels of MET1-2, CMT3, CMT2, DRM2, and DNMT2 were significantly reduced after AzaD treatment. *p < 0.05, **p < 0.01 by Student’s t-test. CK and WT refer to check (CK, Kitaake without AzaD treatment) and WT (wild-type Nipponbare without mutation of MET1-2) plants, respectively