Improving Agricultural Traits While Maintaining High Resistant Starch Content in Rice

Rice

Table 1 Genotypes of rice lines used in this study, and their grain weight-related data

Line	Genotype	Grain weight^a		Rate of increase (%)^b
Line	Genotype	Mean ± SE	%	Rate of increase (%)^b
Kinmaze	ss2a^L SS3a gbss1^L BE2b	19.0 ± 0.3e	100	–
Nipponbare	ss2a^L SS3a gbss1^L BE2b	20.0 ± 0.3de	105	–
Akita 63	ss2a^L SS3a gbss1^L BE2b	28.4 ± 0.3a	149	–
Kasalath	SS2a SS3a GBSS1 BE2b	15.4 ± 0.2f	81	–
EM10 (BC₃)	ss2a^L SS3a gbss1^L be2b	19.4 ± 0.6e	102	181
#4019 (BC₃)	ss2a^L ss3a gbss1^L be2b	21.3 ± 0.3bcde	112	134
#1203A (BC₃)	SS2a SS3a gbss1^L be2b	12.7 ± 0.3g	67	135
#1203B (BC₃)-1	ss2a^L SS3a GBSS1 be2b	28.1 ± 0.4a	148	184
#1203B (BC₃)-2	ss2a^L SS3a GBSS1 be2b	29.0 ± 0.8a	153	190
#1203C (BC₃)-1	SS2a SS3a GBSS1 be2b	22.2 ± 0.6bcd	117	144
#1203C (BC₃)-2	SS2a SS3a GBSS1 be2b	23.6 ± 0.4b	124	153
#1206A (BC₃)	SS2a ss3a gbss1^L be2b	19.1 ± 0.4e	101	145
#1206B (BC₃)	ss2a^L ss3a GBSS1 be2b	22.9 ± 0.5bc	121	132
#1206C (BC₃)	SS2a ss3a GBSS1 be2b	20.7 ± 0.3cde	109	145

^aThe weight (mg) of one dehulled mature grain of each rice line is presented as mean ± standard error (SE; n = 20) as well as a percentage of the seed weight of the wild-type (WT) cultivar, Kinmaze. Increase in seed weight before and after backcrossing was calculated. Different lowercase letters indicate significant differences among the different rice genotypes (P < 0.05; Tukey–Kramer method)
^bRate of increase in seed weight after backcrossing relative to that before backcrossing relative to the values in Table S1. Average seed weight was used where multiple lines with the same genotype were available