Fig. 2
OsIMα1a and OsIMα1b interacting with OsWRKY62.1 and OsWRKY76.1 primarily in the nuclei. a BiFC visualizations of OsWRKY62.1 (W62.1) and OsWRKY76.1 (W76.1) interacting with OsIMα1a and OsIMα1b. W62.1 and W76.1 were fused in frame with YFP N-terminal region (YFPN) and IMαIBB1a and IMαIBB1b were fused with YFP C-terminal region (YFPC). The plasmids indicated were introduced into N. benthamiana leaves through agroinfiltration method. Confocal images were taken 72 h after the treatments. Red fluorescence (dsREDNLS) shows nuclear localization. From left panels to right: YFP images (YFP), dsRED images (RED), and combined YFP and RED in the bright field (Merged). b Co-localization of OsW62.1 with OsIMα1a and OsIMα1b. Plasmid 35S::W62.1-GFP or in combination with plasmid indicated was delivered into N. benthamiana leaves through agroinfiltration. Images were photographed 72 h after the treatments. DAPI (4',6-diamidino-2-phenylindole) for nuclear staining. From left panels to right: GFP, RED, DAPI, and the bright field image combined the fluorescent images (Merged). c Relative quantity of OsWRKY62.1-GFP localized in the nuclei. More than 100 cells from (b) were counted. Significant differences (Duncan's multiple range test; α = 0.05) compared with 35S::W62.1-GFP only are listed in the figure. Bar = 20 µm