Fig. 1
From: Fine-Tuning of the Grain Size by Alternative Splicing of GS3 in Rice
Alternative splicing of GS3 fine-tunes grain size in rice. A Sequencing of PCR products of GS3. B GS3 AS variants are shown by PAGE. Lane 1 and 2 show 40 and 35 cycles, respectively. M, DNA ladder. C Sequence comparison of GS3.1 and GS3.2. D Protein structures of GS3.1 and GS3.2. E Ratio of GS3.2/GS3.1 in different tissues. F GS3.2 expression level under CHX treatment. G Construct of GS3.2m by A-T substitution in 3’ splice site. H Subcellular localization of GS3.1, GS3.2 and GS3.2m in rice protoplasts. I Phenotypic comparison of grain length among GS3.1 and GS3.2 overexpressors. Bar = 1 cm. J Grain length, K grain width and L 1000-grain weight of the genotypes tested. Data are given as mean ± SEM. *p < 0.01; ***p < 0.001. M Interactions between GS3 AS variants and RGB1 tested by yeast-two-hybrid and N by BiFC. O GS3.2 disrupts the interaction between GS3.1 and RGB1 tested by yeast-three-hybrid and P by luciferase activity assay