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Table 2 Mutation types and grain amylose contents (AC) of the first generation transgene-free, homozygous mutants

From: Targeted Deletion of the First Intron of the Wxb Allele via CRISPR/Cas9 Significantly Increases Grain Amylose Content in Rice

Background Mutant line No. of transgene-free plants tested Mutation type AC (%)
KY131 KY131 12 WT 13.8 ± 0.22d
KY131-CR-1 8 M2 22.5 ± 0.07b
KY131-CR-2 6 M2 22.7 ± 0.36b
KY131-CR-3 12 M3 24.3 ± 0.12a
KY131-CR-4 11 M2 21.5 ± 0.40c
X32 X32 12 WT 11.2 ± 0.06d
X32-CR-1 8 M1 23.3 ± 0.37a
X32-CR-2 8 M2 22.0 ± 0.65b
X32-CR-3 12 M2 21.3 ± 0.34c
X32-CR-4 9 M1 23.2 ± 0.25a
X55 X55 12 WT 28.1 ± 0.68a
X55-CR-1 8 M2 28.1 ± 0.06a
X55-CR-2 6 M2 28.4 ± 0.69a
X55-CR-3 7 M2 28.3 ± 0.64a
X55-CR-4 10 M2 27.9 ± 0.39a
X35 X35 12 WT 25.5 ± 0.68a
X35-CR-1 9 M3 25.5 ± 0.43a
X35-CR-2 12 M2 25.3 ± 0.34a
  1. M1, M2, M3 represent mutants with 1041 bp, 1042 bp, 1043 bp deletion, respectively (Fig. 1D). AC values reported are mean ± SD. Different letters following the AC mean values stands indicate significant differences (P < 0.05)