Targeted Deletion of the First Intron of the Wxb Allele via CRISPR/Cas9 Significantly Increases Grain Amylose Content in Rice

Rice

Table 2 Mutation types and grain amylose contents (AC) of the first generation transgene-free, homozygous mutants

Background	Mutant line	No. of transgene-free plants tested	Mutation type	AC (%)
KY131	KY131	12	WT	13.8 ± 0.22d
	KY131-CR-1	8	M2	22.5 ± 0.07b
	KY131-CR-2	6	M2	22.7 ± 0.36b
	KY131-CR-3	12	M3	24.3 ± 0.12a
	KY131-CR-4	11	M2	21.5 ± 0.40c
X32	X32	12	WT	11.2 ± 0.06d
	X32-CR-1	8	M1	23.3 ± 0.37a
	X32-CR-2	8	M2	22.0 ± 0.65b
	X32-CR-3	12	M2	21.3 ± 0.34c
	X32-CR-4	9	M1	23.2 ± 0.25a
X55	X55	12	WT	28.1 ± 0.68a
	X55-CR-1	8	M2	28.1 ± 0.06a
	X55-CR-2	6	M2	28.4 ± 0.69a
	X55-CR-3	7	M2	28.3 ± 0.64a
	X55-CR-4	10	M2	27.9 ± 0.39a
X35	X35	12	WT	25.5 ± 0.68a
	X35-CR-1	9	M3	25.5 ± 0.43a
	X35-CR-2	12	M2	25.3 ± 0.34a

M1, M2, M3 represent mutants with 1041 bp, 1042 bp, 1043 bp deletion, respectively (Fig. 1D). AC values reported are mean ± SD. Different letters following the AC mean values stands indicate significant differences (P < 0.05)