Table 1 The putative off-target event in the osspl4 mutant lines
From: The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
Target site | Putative off-target site | Sequence of putative off-target site | Region | No. of plants | No. of plants with mutations | Mutation rate (%) |
|---|---|---|---|---|---|---|
OsSPL4-OFF1 | Chr7:19100187-19100209 | AGGTGCCAGGTGGAGAGGTGCGG | CDS | 10 | 2 | 20% |
OsSPL4-OFF2 | Chr8:25274878-25274900 | CGGTGCCAGGTGGAGGGGTGCGG | CDS | 10 | 1 | 10% |
OsSPL4-OFF3 | Chr9:18918476-18918498 | AGGTGCCAGGTGGAGGGTTGCGG | CDS | 10 | 0 | 0 |
OsSPL4-OFF4 | Chr8:167437-167459 | TGGTGCTAGGAGGAAGGGTTTGG | Intergenic | 10 | 0 | 0 |
OsSPL4-OFF5 | Chr9:16055179-16055201 | AGAGGCCTGGTGGAAAGGTGAGG | Intron | 10 | 0 | 0 |
OsSPL4-OFF6 | Chr1:18039404-18039426 | AAGCGCCATGTGGAGGGGTGTGG | Intergenic | 10 | 0 | 0 |