Fig. 4

Phenotypic and metal analysis of OsLCT2-transgenic lines and the wild-type (WT) grown in a paddy field. a Expression levels of OsLCT2 in roots of overexpression lines relative to that in the WT by qRT-PCR. b Structure of OsLCT2 and mutated sequences of OsLCT2 knockout lines. The differences in target nucleotide sequence between the WT and two mutant alleles are highlighted in red. To the right of the sequence, ‘+’ signs indicate the number of nucleotides inserted. c, d Phenotypes of the WT and overexpression lines (c) and knockout lines (d) at the maturity stage. Scale bar, 18 cm. e Grain yield of transgenic lines. f–j The concentrations of Cd (f), Mn (g), Fe (h), Zn (i) and Cu (j) in brown rice of transgenic lines. k–o The concentrations of Cd (k), Mn (l), Fe (m), Zn (n) and Cu (o) in the straw of transgenic lines. Data show means ± SD of three biological replicates. One asterisk above bars indicates significant difference from the WT at P < 0.05 (Student’s t-test). DW, dry weight