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Rice
Fig. 8 | Rice

Fig. 8

From: SDG712, a Putative H3K9-Specific Methyltransferase Encoding Gene, Delays Flowering through Repressing the Expression of Florigen Genes in Rice

Fig. 8

Chromatin immunoprecipitation qPCR (ChIP-qPCR) assays of Ehd1, RFT1 and Hd3a loci. Leaves of 50-day-old plants grown in fields were collected 3 h after dawn. ChIP assays were performed using antibodies against histone H3K9 dimethylation (H3K9me2), and qPCR (Quantitative PCR) was used to analyze the levels of methylation of Ehd1, RFT1 and Hd3a. A, the structure of Ehd1, RFT1 and Hd3a. Filled boxes denote exons and lines denote introns or 5′ upstream regions of corresponding genes. Arrows denote the transcription direction of genes. The numbers 1–10 denote the position of primers. B-D, qPCR results of Ehd1, RFT1 and Hd3a loci, respectively. OsActin1 was used as an internal standard for normalization. WT, Zhonghua11. Three biological replicates were performed. Asterisks indicate statistically significant differences of t-test (*, P < 0.05)

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