Fig. 4
Obtainment and phenotypic analysis of SDG712 overexpression transgenic lines. A, Structure of the vector used for overexpression of SDG712. 35S, 2X35S, Cauliflower Mosaic Virus (CaMV) 35S promoter sequences; NptII, neomycin phosphotransferase II gene; TOCS, terminator of octopine synthase gene; RB, right border; LB, left border. B, Expression of SDG712 in transgenic plants overexpressing SDG712 (SDG712-OX) by qRT-PCR. WT, Zhonghua11; OX-1, OX-2, transgenic lines. C, The phenotype of SDG712-OX under natural long-day condition. Bar = 20 cm. D, Heading-date of SDG712-OX lines under natural long-day (NLD) and natural short-day (NSD) conditions. Values are shown as means ± SD. N ≥ 30 individual plants. E, Heading-date of SDG712-OX-1 plants under artificial day-length conditions. The wild type Zhonghua11 and SDG712-OX1 transgenic plants were grown in Tianjin. Two-week-old plants were subjected to light-controlled treatments with 9 h light/ 15 dark for SD condition and 15 h light/9 h dark for LD condition. The heading-date was recorded after heading. Values are shown as means ± SD. N ≥ 20 individual plants. SD, artificial short-day condition; LD, artificial long-day condition. Asterisks in (D) and (E) indicate statistically significant differences of t-test (**, P < 0.01)