Fig. 3

The morphology and weight of seeds from T₀ transgenic plants. (a) The seed setting rates of the individual T₀ transgenic plants. (b) The de-hulled grains from eight T₀ transgenic plants. Grains with normal endosperm and grains with shrunken endosperm are shown. (c, d) 1000-grain weight of seeds from T₀ transgenic plants silencing OsAGPL2 (c) and OsAGPS2 (d.) The seeds from each individual T₀ plant were sorted by a weight sorter into the heavy-weight and light-weight groups. The 1000-grain weight was measured for each group. (e) The de-hulled heavy and light grains from A1-4. The arrows indicate two molded grains. (f-h) The OsAGPL2 and OsAGPL2 mRNA levels in the light and heavy seeds from the corresponding gene-silencing plants. The OsAGPL2 and OsAGPS2 transcript levels in the light seeds were compared with that in the wild type seeds (f, g) or that in the heavy seed (h). The transcript levels were determined using qRT-PCR with the ubiquitin gene as internal control. Data are shown as means ± SD (n = 3). Bar: (b, e) = 1 cm