Fig. 1
From: Construction of a weight-based seed sorting system for the third-generation hybrid rice
Design of the weight-based seed sorting system. (a, b) Target sites and the corresponding amiRNA sequences for silencing OsAGPL2 (a) and OsAGPS2 (b). Each amiRNA gene was driven by OsAGPL2 promoter (proOsAGPL2) and OsRA16 promoter (proOsRA16). The eight constructs were named as A1, A2, B1, B2, C1, C2, D1, and D2. (c, d) The spatial expression patterns of OsAGPL2 (c) and OsRA16 (d). The gene expression levels were determined by qRT-PCR with Actin gene as internal control. Data are shown as means ± SD (n = 3). DAP, day after pollination. (e) Diagram showing the three function modules. ProOsNP1-OsNP1 is for fertility restoration, proOsLSP3-ASP1 + OsAA-In2-1 is for pollen inactivation, and promoter-amiRNA-PINII TERM is for silencing OsAGPL2 or OsAGPS2 gene. In2-1 and PINII TERM are transcriptional terminators of the maize In2-1 gene and the potato PINII (Proteinase Inhibitor II) gene, respectively (Chang et al. 2016)