Unique Glutelin Expression Patterns and Seed Endosperm Structure Facilitate Glutelin Accumulation in Polyploid Rice Seed

Rice

Table 1 Identification of varying expression levels of glutelins and protein disulfide isomerase-like 1–1 (PDIL1–1) using tandem mass tags (≥1.5-fold) between 9311-2x and 9311-4x (harvested in 2019) at 25 days after pollination

Identified Protein	Coverage^a %	Theor:Mw(kDa)^b	Peptides	Locus No.	Ratio	P-value	Gene name
Similar to Glutelin type-A1	49.5	56.239	27	Os01g0762500	1.700	0.000117	GluA-1
Glutelin type-A2	84.5	25.522	25	Os10g0400200	1.644	0.0000011	GluA-2
Glutelin type-A3	57.1	56.014	28	OS03g0427300	2.185	0.0000171	GluA-3
Similar to Glutelin type-B2	51.5	56.062	35	Os02g0249600	1.795	0.0000766	GluB-2
Similar to Glutelin type-B4	66.8	56.823	40	Os02g0268300	2.076	0.00402	GluB-4
GluB-5, glutelin precursor	40.7	56.835	27	Os02g0242600	2.417	0.0000007	GluB-5
Glutelin type-B5	37.3	54.693	25	Os02g0242600	1.654	0.000215	GluB-7
Similar to Glutelin type-B5	40.7	56.835	27	Os02g0242600	2.417	0.0000007	GluB-7
Glutelin type-D1	43.8	53.252	26	Os02g0249000	1.999	0.0000002	GluD-1
Protein disulfide isomerase-like 1–1	67.8	56.854	41	Os11g0199200	1.594	0.00000286	PDIL1–1

^aCoverage denotes the percentage of protein sequence covered by the identified peptides
^btheoretical molecular weight (MW)