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Fig. 5 | Rice

Fig. 5

From: Unique Glutelin Expression Patterns and Seed Endosperm Structure Facilitate Glutelin Accumulation in Polyploid Rice Seed

Fig. 5

Accumulation patterns of various glutelin polypeptides in rice with varying ploidy levels. Total proteins in rice seeds from 3, 5, 7, 9, 11, 13, 17, 21, and 25 DAF in 9311-2x and 9311-4x (harvested in 2019) were subjected to immunoblotting analysis with anti-GluA-1, anti-GluA-2, anti-GluB-1, anti-GluB-2, anti-GluB-4/5, anti-GluC-1, and anti-GluD-1 antibodies. Each lane consists of rice seeds with similar weights. Pro-glutelins and glutelin acidic subunits are indicated by black arrowheads. β-tubulin was used as a loading control. Three independent experiments were performed and data are shown

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